Human kidney organoids produce functional renin

Abstract

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Which developments in urology have you missed amongst all the COVID‐19 papers?

“…27 For the nephrologist, functioning organoids that selforganise into tubules and other renal structures produce physiologically active molecules such as renin. 28 More recently, kidney organoids and tumours have been used to test drug treatments. For example, a biobank of tumour and normal kidney organoids from children with a variety of kidney tumours has been used to identify patient-specific drug sensitivities.…”

Generation of the organotypic kidney structure by integrating pluripotent stem cell-derived renal stroma

“…Thus, the generation of renin cells and mesangial cells is a significant step toward functionality of the kidney. Recently, cAMP stimulation of conventional kidney organoids was shown to induce renin production 40 . Combination of our protocol with cAMP stimulation may further increase the induction efficiency for renin cells.…”

Kidney organoids: current knowledge and future directions

“…RNA sequencing analysis showed that these organoids resembled the first trimester of fetal kidney tissue. Several studies utilized this protocol to conduct in vivo transplantations (Koning et al 2018), developmental research (Bantounas et al 2018), renal disease modeling (Forbes et al 2018;Howden et al 2018), scale-up of organoid generation (Kumar et al 2019), encapsulating kidney organoids (Geuens et al 2021), and bioprinting technology (Lawlor et al 2021) and to study the role of the renin-angiotensin system (Shankar et al 2021). Bonventre's laboratory created a protocol that generated NPCs with 75-92% efficiency in 9 days.…”