Human keratinocyte stem cells survive for months in sodium chloride and can be successfully transplanted

Olszewski, Waldemar L.; Mościcka, M; Zolich, D; Machowski, Z

doi:10.1016/j.transproceed.2004.12.174

Cited by 11 publications

(10 citation statements)

References 3 publications

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“… 2 , 3 Some authors have shown that human epidermal SC can survive in a dehydrated state in sodium chloride for months and after transplantation give rise to keratinocyte progenies. 13 …”

Section: Discussionmentioning

confidence: 99%

“…One of the characteristics of adult progenitor or SC is a relative resistance to noxious environments. 2 , 3 , 13 UVB irradiation of an acute damaging dose can be insulting to skin 4 but epidermal SC may be resistant for the purpose of self-maintenance. Therefore we tried to determine whether the integrin α6+p63+ cell fraction representative of epidermal progenitor or SC is increased after UVB irradiation and to clarify the hypothesis that epidermal SC are resistant to UVB damage.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Increase of integrin α6+p63+ cells after ultraviolet B irradiation in normal human keratinocytes

Park

Han²,

Chang

et al. 2009

Dermatol Reports

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Epidermal stem cells (SC) are believed to be resistant to environmental damage for the purpose of self renewal. Most promising SC markers include integrin α6 and p63. The aim of our study was to determine whether the integrin α6+p63+ cell fraction representative of the epidermal progenitor or SC is increased after ultraviolet B (UVB) irradiation and to clarify the hypothesis that epidermal SC are resistant to high-dose UVB damage. We irradiated early passage normal human keratinocytes (NHK) with 0, 25, 50, and 100 mJ/cm2 UVB. The percentage of cell death was calculated. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting analyses were performed to identify integrin α6 and p63, and flow cytometry analysis with integrin α6 and p63 antibodies was done. After 50 and 100 mJ/cm2 UVB, integrin α6+p63+ cells were found to be much increased by fluorescence-activated cell sorting. Expression of integrin α6 and p63 was increased in NHK after UVB irradiation, which was shown with real-time RT-PCR and western blotting analyses. We concluded that an increase of integrin α6+p63+ cells after high-dose UVB may suggest that the putative progenitor or SC are resistant to UVB irradiation.

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“… 2 , 3 Some authors have shown that human epidermal SC can survive in a dehydrated state in sodium chloride for months and after transplantation give rise to keratinocyte progenies. 13 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Increase of integrin α6+p63+ cells after ultraviolet B irradiation in normal human keratinocytes

Park

Han²,

Chang

et al. 2009

Dermatol Reports

View full text Add to dashboard Cite

show abstract

“…These adult stem cells might have been used in the studies claiming "re-programming" of adult differentiated fibroblasts. Stem cells in the skin have been reported [58,[79][80][81][82][83][84]. In addition, it must been stressed that our presumptive adult stem cells were characterized as stem cells because they had no functional GJIC, prior to their being characterized as expressing Oct-4.…”

mentioning

confidence: 95%

Commentary: “Re-Programming or Selecting Adult Stem Cells?”

Trosko

2008

Stem Cell Rev

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The recent observations that embryonic stemness-associated genes could assist in the "de-differentiation" of adult skin fibroblast cells to "embryonic-like stem cells", using the "somatic cell nuclear transfer" techniques, have been interpreted as indicating a "re-programming" of genes. These reports have demonstrated a "proof of principle" approach to by-pass many, but not all, of the ethical, scientific and medical limitations of the "therapeutic cloning" of embryonic stem cells from embryos. However, while the interpretation that real "re-programming" of all those somatic fibroblastic differentiation genes might be correct, there does exists an alternative hypothesis of these exciting results. Based on the fact that multipotent adult stem cells exist in most, if not all, adult organs, the possibility exists that all these recent "re-programming" results, using the somatic nuclear transfer techniques, actually were the results of transferred rare nuclear material from the adult stem cells residing in the skin of the mouse, monkey and human samples. An examination of the rationale for this challenging hypothesis has been drawn from the hypothesis of the "stem cell theory of cancer", as well as from the field of human adult stem cells research.

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“…Furthermore, the calcium-dependent desmosome formation was affected by the sodium-regulated keratinization in frog skin cultures [ 24 ]. In addition, human keratinocytes stem cells survive for months in sodium chloride [ 25 ]. Thus, PZ may regulate keratinocytes by targeting sodium-glucose transport.…”

Section: Discussionmentioning

confidence: 99%

Phlorizin, an Active Ingredient of Eleutherococcus senticosus, Increases Proliferative Potential of Keratinocytes with Inhibition of MiR135b and Increased Expression of Type IV Collagen

Choi

Nam

Lee

et al. 2016

Oxidative Medicine and Cellular Longevity

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E. senticosus extract (ESE), known as antioxidant, has diverse pharmacologic effects. It is also used as an antiaging agent for the skin and phlorizin (PZ) is identified as a main ingredient. In this study, the effects of PZ on epidermal stem cells were investigated. Cultured normal human keratinocytes and skin equivalents are used to test whether PZ affects proliferative potential of keratinocytes and how it regulates these effects. Skin equivalents (SEs) were treated with ESE and the results showed that the epidermis became slightly thickened on addition of 0.002% ESE. The staining intensity of p63 as well as proliferating cell nuclear antigen (PCNA) is increased, and integrin α6 was upregulated. Analysis of ESE confirmed that PZ is the main ingredient. When SEs were treated with PZ, similar findings were observed. In particular, the expression of integrin α6, integrin β1, and type IV collagen was increased. Levels of mRNA for type IV collagen were increased and levels of miR135b were downregulated. All these findings suggested that PZ can affect the proliferative potential of epidermal cells in part by microenvironment changes via miR135b downregulation and following increased expression of type IV collagen.

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Human keratinocyte stem cells survive for months in sodium chloride and can be successfully transplanted

Cited by 11 publications

References 3 publications

Increase of integrin α6+p63+ cells after ultraviolet B irradiation in normal human keratinocytes

Increase of integrin α6+p63+ cells after ultraviolet B irradiation in normal human keratinocytes

Commentary: “Re-Programming or Selecting Adult Stem Cells?”

Phlorizin, an Active Ingredient of Eleutherococcus senticosus, Increases Proliferative Potential of Keratinocytes with Inhibition of MiR135b and Increased Expression of Type IV Collagen

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