Human Intraplatelet 5-Hydroxytryptamine Is Correlated With Mean Platelet Survival Time

Aranda, Eduardo; Pereira, Jaime; Ajenjo, Cristina; Prieto, Carolina; Sepúlveda, Sebastián; Mezzano, Diego

doi:10.1016/0049-3848(96)00162-4

Cited by 5 publications

(6 citation statements)

References 15 publications

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“…Earlier and present results thus indicate that thrombin‐induced serotonin release is a sensitive marker of platelet viability and that platelets with high endogenous serotonin have a high thrombin‐induced release of endogenous serotonin after up to 10 days of storage, indicating a better function ( Hervig et al ., 1990 ; Hervig & Farstad, 1996). Our results are also in agreement with reported findings that platelets with a high endogenous seroronin have a longer in vivo life ( Aranda et al ., 1996 ).…”

Section: Discussionsupporting

confidence: 94%

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The swirling phenomenon in stored platelets is influenced by their endogenous serotonin

Hervig

Bakken

Farstad

1999

Transfusion Medicine

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The presence of the swirling phenomenon is useful to define platelet concentrates that are suitable for transfusion. If it is possible to identify donor-related factors which are related to persisting swirling during storage, it is possible to select platelet donors. Endogenous platelet serotonin content is stable and easily measured and related to agonist-induced serotonin secretion. During a 3-month period, the swirling in 825 single donor platelet concentrates was controlled before issue. Endogenous serotonin, % serotonin release and swirling were tested in 21 concentrates with poor or no swirling during storage. Sixty-three concentrates were randomly selected from the routinely prepared platelet concentrates and were routinely tested with the same analyses on days 1 and 7. To evaluate an obvious effect of endogenous serotonin on the swirling phenomenon, eight platelet concentrates prepared from buffy coat, each from four donors, were divided. One part was stored in the presence of 8.5 micromol serotonin L-1, and analysed as the control concentrates. The endogenous serotonin content in the 'low- swirling' concentrates was significantly lower than in the control group (P < 0.001). PCO2 and pH were significantly lower, and PO2 and MPV significantly higher than in the controls. In the control group, swirling after 7 days was significantly correlated with serotonin release. In the eight buffy-coat concentrates enriched in endogenous serotonin, both swirling and the percentage serotonin release were improved after storage for 10 days, compared with nonenriched concentrates. This study suggests that endogenous serotonin content and serotonin release are factors that may be of significance concerning preservation of the swirling phenomenon in platelet concentrates during storage.

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Section: Discussionsupporting

confidence: 94%

“…The intrapersonal variations in the content of platelet serotonin over time, at least for several months, are small ( Hervig et al ., 1996 ). Also, endogenous serotonin influences platelet functions, assayed as uptake or release of serotonin in vitro ( Beving et al ., 1983 ; Hervig & Farstad, 1996) and viability in vivo ( Aranda et al ., 1996 ).…”

mentioning

confidence: 99%

The swirling phenomenon in stored platelets is influenced by their endogenous serotonin

Hervig

Bakken

Farstad

1999

Transfusion Medicine

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“…The latter may explain the lack of difference in PS expression among canine platelet density subpopulations, despite the higher 5-HT content of LD platelets, denoting their increased mean age. The process of 5-HT accumulation by aging platelets appears to be continuous and progressive throughout their physiological life-span (15), whereas the loss of membrane phospholipid asymmetry might be a phenomenon occurring just before the platelets are removed from circulation. It is very unlikely that rise in PS expression was the consequence of thrombocytopenia since no other markers of platelet activation were found and, the findings in the model of suppressed thrombopoiesis were consistent with those in platelet density subpopulations where platelets are studied in steady state.…”

Section: Discussionmentioning

confidence: 99%

“…As platelets age in circulation, they under-go a series of changes, many of which can be regarded as signs of a senescence process. In this sense, it has been shown that platelet aging is associated with changes in density (2)(3)(4)(5)(6)(7)(8)(9), size (10), ␣-granule content (11), platelet-associated IgG (12,13), HLA expression (14) and serotonin (5-hydroxytryptamine, 5-HT) content (4,15). The relationship between platelet density and age has been a matter of controversy.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, changes in 5-HT content associated with platelet-aging have been extensively studied. Observations in humans and dogs have shown unequivocally that platelets accumulate 5-HT while aging in circulation (4,15,16); this has lead to propose the 5-HT content as a useful marker of platelet age (15). However, despite their consistent association with the phenomenon of platelet aging, it is unlikely that any of these changes constitute the senescence signal on the platelet surface recognized by the macrophages.…”

Section: Introductionmentioning

confidence: 99%

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Platelet Aging In Vivo Is Associated with Loss of Membrane Phospholipid Asymmetry

Pereira

Palomo²,

Ocqueteau³

et al. 1999

Thromb Haemost

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SummaryThe mechanism(s) involved in the clearance of senescent platelets are largely unknown. The loss of membrane phospholipid (PL) asymmetry, with phosphatidylserine (PS) exposure appears to be an important signal for the ingestion by macrophages of apoptotic nucleated cells and it has also been suggested as a signal for the removal of aged erythrocytes. Accordingly, it seems possible that the clearance of normal aged platelets from circulation might be triggered by PS exposure. To investigate this, we determined PS exposure in human aging platelets taking advantage of the relationship between platelet density and platelet age and in dog platelets in a model of platelet aging in vivo. PS exposure was determined in two experimental conditions: 1) human platelet density subpopulations obtained by centrifugation in arabinogalactan gradients; 2) circulating canine platelets during decline in platelet count after suppression of thrombopoiesis following estradiol injection. PS exposure was determined by flow cytometry after labeling the cells with FITC-conjugated annexin V. The proportion of human platelets with exposed PS was significantly higher in high density (HD) platelets compared to low density (LD) platelets (11.3 ± 8.0% vs 5.2 ± 3.7%; p <0.05, respectively). In dogs, the proportion of cells with exposed PS rises dramatically with age, from 3.1 ± 0.4% before to 17.7 ± 12.3% ten days after estradiol injection. These findings suggest that platelet aging is associated with loss of phospholipid asymmetry and PS exposure on the outer leaflet of cell membrane, which may play an important role in the recognition and subsequent removal of senescent platelets.

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Serotonin secretion by blood platelets: accuracy of high-performance liquid chromatography-electrochemical technique compared with the isotopic test and use in a clinical laboratory

Aranda,

Iha,

Solari

et al. 2023

Research and Practice in Thrombosis and Haemostasis

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Human Intraplatelet 5-Hydroxytryptamine Is Correlated With Mean Platelet Survival Time

Cited by 5 publications

References 15 publications

The swirling phenomenon in stored platelets is influenced by their endogenous serotonin

The swirling phenomenon in stored platelets is influenced by their endogenous serotonin

Platelet Aging In Vivo Is Associated with Loss of Membrane Phospholipid Asymmetry

Serotonin secretion by blood platelets: accuracy of high-performance liquid chromatography-electrochemical technique compared with the isotopic test and use in a clinical laboratory

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