Human intestinal mucosa-associated Lactobacillus and Bifidobacterium strains with probiotic properties modulate IL-10, IL-6 and IL-12 gene expression in THP-1 cells

Abstract

Lactobacilli and bifidobacteria are considered one of the permanent genera of the physiological human intestinal microbiota and represent an enormous pool of potential probiotic candidates. Approximately 450 isolates of presumptive Lactobacillus or Bifidobacterium strains were obtained from bioptic samples of colonic and ileal mucosa from 15 adolescents aged 12 to 18 years. On the basis of randomly amplified polymorphic DNA (RAPD)-PCR analysis, 20 strains were selected for further taxonomic classification and characterisation, as well as assessment of probiotic properties and safety. Importantly, selected strains showed the capability of colonising different parts of the intestine. The most frequently isolated species was Lactobacillus paracasei followed by Lactobacillus fermentum. The majority of isolates were susceptible to antimicrobials of human and veterinary importance, however, tetracycline and/or erythromycin resistance was observed in Lactobacillus plantarum and L. fermentum strains. Thirteen strains were able to ferment more than 19 different carbon sources and three out of five tested strains exerted antagonistic activity against several different indicator strains. Two Lactobacillus isolates (L. paracasei L350 and L. fermentum L930 bb) and one Bifidobacterium isolate (Bifidobacterium animalis subsp. animalis IM386) fulfilled in vitro selection criteria for probiotic strains and exhibited strong downregulation of pro-inflammatory cytokines IL-6 and IL-12 and upregulation of anti-inflammatory IL-10. The selected strains represent suitable candidates for further studies regarding their positive influence on host health and could play an important role in ameliorating the symptoms of inflammatory bowel diseases.

A randomized, double-blind, placebo-controlled, clinical trial on probiotic soy milk and soy milk: effects on epigenetics and oxidative stress in patients with type II diabetes

“…Scientists believe that using soy foods like soymilk with probiotic can improve the protective effect of either one of them because: (1) Soymilk can act as a substrate for probiotics; therefore, it increases epigenetic effects of probiotics, and (2) probiotic changes isoflavones to other active metabolites and increases their absorption through a change in the intestinal environment (Teh et al 2010;Tsangalis et al 2005;Zielinska et al 2014). Lactobacillus plantarum A7, a strain of probiotic, is also known to change the expression of many genes and has antioxidant effects, but there are no clear evidences of the mechanism of these effects (Citar et al 2014;Li et al 2014). …”

Efficacy of probiotics on anxiety-A meta-analysis of randomized controlled trials

“…Probiotics, defined as "live microorganisms that would bring health benefit to the host when administered in proper amount and frequency (Hill et al, 2014)," are deemed to have beneficial effects on emotion and behavior through multiple mechanisms, which serve as the biological basis of "gut-brain axis." Specifically, probiotics may help to maintain the function of intestinal mucosal barrier, reduce the level of proinflammatory cytokines (IL-1 , IL-6, and TNF-, among others; Ait-Belgnaoui et al, 2012;Gareau, Silva, & Perdue, 2008;Luo et al, 2014) and cells (Marcos et al, 2004), and elevate the level of anti-inflammatory cytokines (IFN-, IL-10, and TGF-, among others; Citar et al, 2015;Kwon et al, 2010). Specifically, probiotics may help to maintain the function of intestinal mucosal barrier, reduce the level of proinflammatory cytokines (IL-1 , IL-6, and TNF-, among others; Ait-Belgnaoui et al, 2012;Gareau, Silva, & Perdue, 2008;Luo et al, 2014) and cells (Marcos et al, 2004), and elevate the level of anti-inflammatory cytokines (IFN-, IL-10, and TGF-, among others; Citar et al, 2015;Kwon et al, 2010).…”

Bifidobacterium animalis ssp. lactis Bb12 induces IL-10 through cell membrane-associated components via TLR2 in swine

“…Citar et al . () found an increase in the expression of IL‐10 and the repression of IL‐6 and IL‐12 β induced by several bifidobacteria strains on the monocytic THP‐1 human cell line in the presence or absence of LPS (Citar et al . ).…”