Human interleukin 2 receptor beta-chain gene: chromosomal localization and identification of 5' regulatory sequences.

Abstract

Interleukin 2 (IL-2) binds to and stimulates activated T cells through high-affinity IL-2 receptors (IL-2Rs). Such receptors represent a complex consisting of at least two proteins, the 55-kDa IL-2Ra chain and the 70-kDa IL-2R chain. The low-affinity, IL-2Ra chain cannot by itself transduce a mitogenic signal, whereas IL-2 stimulates resting lymphocytes through the intermediate-affinity, IL-2Rf receptor.We report here identification of the genomic locus for IL-2R,3. The exons are contained on four EcoRI fragments of 1.1, 9.2, 7.2, and 13.7 kilobases. The 1.1-kilobase EcoRI fragment lies at the 5'-most end of the genomic locus and contains promoter sequences. The promoter contains no TATA box-like elements but does contain the d(GT). class of middle repetitive elements, which may play an interesting regulatory role. The IL-2Rf3 gene is localized to chromosome 22qll.2-ql2, a region that is the locus for several lymphoid neoplasias.

Bcl-3 Expression Promotes Cell Survival following Interleukin-4 Deprivation and Is Controlled by AP1 and AP1-Like Transcription Factors

“…The lack of this motif has also been observed in a number of genes whose products have house- (17,39,42). Analysis of the Bcl-3 promoter revealed the presence of three transcription start sites; the initiation of gene transcription at multiple sites is consistent with the lack of a canonical TATA box in the promoter.…”

Interleukin-2 receptor-γ-dependent endocytosis depends on biotin in Jurkat cells

“…This hypothesis is consistent with decreased (apparent) secretion of IL-2 by biotin-supplemented cells (because of increased endocytosis) despite the beneficial effects of biotin supplementation on immune function (because of increased IL-2 signaling activity). A human lymphoid cell line (Jurkat cells) that expresses IL-2R␣ and IL-2R␥ (12,14) was used to test this hypothesis.…”

Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products.

“…Similar to IL-2Ra, IL-2R13 expression is induced after activation by phytohemagglutinin (18), anti-CD3 (6), and high-dose IL-2 (38). The IL-2R,B 5' regulatory region lacks canonical TATA and CAAT motifs but directs transcription from one major and four minor initiation sites (14,37). There are no NF-KB or CArG motifs, sites which are critical for IL-2Ra promoter activation in response to various stimuli (reviewed in references 16 and 25).…”

“…1B). Although Jurkat cells do not express endogenous IL-2Rt, the IL-2RP promoter-CAT construct is active in these cells (14), indicating that they contain the nuclear JMXH,-857/+97 was 14-fold more active than the JOCAT control vector; expression modestly increased following PMA treatment (Fig. 1B) To further characterize the IL-2RP 5' regulatory region, we subcloned nested fragments spanning the -857 to -34 region ( Fig.…”

“…A human IL-2R, promoter construct with a 5' endpoint of -857 was previously shown to possess promoter activity (14). To delineate cis-acting elements and trans-acting factors which regulate IL-2R1 expression, we first examined expression of the full-length promoter (JMXHI-857/+97) and eight nested 5' deletion promoter-CAT constructs (Fig.…”