Human Interleukin 2: Physiology, Biochemistry, and Pathophysiology in Lymphoblastic Leukemias and Immunodeficiency Syndromes

Welte, Karl; Venuta, Salvatore; Wang, C. Y.; Feldman, S.; Ciobanu, Niculae; Kruger, G.; Feickert, H. J.; Merluzzi, Vincent J.; Flomenberg, Neal; Moore, M. A. S.; Mertelsmann, R

doi:10.1007/978-3-642-68761-7_72

Cited by 8 publications

(1 citation statement)

References 17 publications

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“…It is conceivable that pre-incubation of T-cells with the OKT8 antibody may inhibit the mitogenic response of OKT8 + cells and provides a negative response to 11-2 (Welte et al 1983). In contrast, the proliferation of OKT8 + cells was not changed by the addition of 11-2 in controls and patients.…”

Section: Discussionmentioning

confidence: 99%

Impaired T- and B-cell functions in patients with Hodgkin's disease

Bergmann

Mitrou

Demmer-Dieckmann

et al. 1987

Cancer Immunol Immunother

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The mitogenic response of T-cell subsets, the production of interleukin-1 (Il-1) and interleukin-2 (Il-2) and in vitro immunoglobulin production was investigated in patients with Hodgkin's disease (HD). The mitogenic response of mononuclear cells (MNC) and the OKT4+ and OKT8+ subsets was greatly reduced in advanced disease stages and could only partially be restored with exogeneous Il-2. In untreated patients with HD--except those with highly advanced disease--the OKT4+ lymphocytes showed normal response to phytohemagglutinin in contrast to the MNC suggesting inhibiting agents or cells within in the MNC. These findings corresponded to reduced Il-2 synthesis of MNC, whereas isolated OKT4+--cells produced normal or elevated amounts of Il-2. MNC or monocytes produced normal or even higher amounts of lipopolysaccharide-induced Il-1 than controls. The results do not confirm a defect in this component of the interleukin system in HD. The immunological impairment was not limited to the T-cell system but involved B-cell activation and differentiation as well. The pokeweed mitogen-induced IgM, IgG and IgG production was highly suppressed in untreated HD, whereas the MNC of previously treated patients produced subnormal amounts of immunoglobulin in vitro. It is not yet clear whether this defect is T-cell-mediated or primarily a B-cell deficiency.

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Section: Discussionmentioning

confidence: 99%

Impaired T- and B-cell functions in patients with Hodgkin's disease

Bergmann

Mitrou

Demmer-Dieckmann

et al. 1987

Cancer Immunol Immunother

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Einfluß von Human-Interleukin-2 auf die zelluläre Immunität bei Hämophilie A- und von Willebrand-Patienten im Kindesalter

Kreuz

Ebener

Kornhuber

1986

2. Rundtischgespräch Therapiebedingte Infektionen Und Immundefekte Bei Hämophilen

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T cell surface antigen expression on lymphocytes of patients with AIDS during in vitro mitogen stimulation

Munn

Reuben²,

Hersh

et al. 1984

Cancer Immunol Immunother

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Surface marker expression on peripheral blood mononuclear cells (PBMC) was evaluated daily in PHA- and PWM-stimulated cultures of eight AIDS patients and eight normals. Before culture, the patients' cells showed the characteristic decrease in OKT 4+ cells (normals 40.4%, patients 22.3%; P less than 0.001), increase in OKT 8+ cells (normals 27.6%, AIDS 38.4%; P = 0.002), increase in OKT 10+ cells (normals 15.5%, AIDS 42.8%; P = 0.002), and increase in HLA-DR+ cells (normals 11.4%, AIDS 28.7%; P = 0.01). The percentage of OKT 11+ cells remained unchanged, while the percentage of OKT 3+ cells dropped over the first 2 days in PHA but not in PWM cultures of both groups (PHA: normals 69.8% to 35.1%; P = 0.001, AIDS 56.5 to 38.5%; P = 0.001, PWM: normals 62.8%-65.9%, AIDS 66.8% to 63.9%), and recovered in both groups by day 5. In PWM cultures OKT 3+ cells increased significantly in normals but not in AIDS (normals 62.6%-77.7%; P = 0.04, AIDS 61.8 to 48.7%). OKT 4 expression decreased in normal PHA cultures after 1 day (38.9% to 29.6%; P = 0.05) and then recovered by day 5. Its expression increased in AIDS PHA cultures by day 5 (18.0%-41.1%; P less than 0.001). The final percentage of OKT 4+ cells in AIDS cultures was within the normal range (35.0%-49.0%). OKT 8 expression increased in both study groups after PHA stimulation (normals 29.5%-50.4%; P = 0.002, AIDS 37.4%-50.7%; P = 0.02) and in normals but not AIDS after PWM stimulation (normals 28.9%-35.5%; P = 0.004, AIDS 38.5%-35.6%). Because of the relative changes in expression of OKT 4 and OKT 8, the 4/8 ratio declined in the normal PHA cultures (1.89 to 1.03; P = 0.1) and increased in the AIDS cultures (0.68-1.18; P = 0.09). Also, the sum of OKT 4+ and OKT 8+ cells in PHA cultures increased from 68% to 94% whilst expression of OKT 11 remained unchanged, indicating co-expression of these antigens on individual cells. Both PHA-and PWM-stimulated normal cells showed an increase in OKT 10 (PHA 16.0%-53.4%; P = 0.01, PWM 16.1%-33.9%; P = 0.03) and HLA-DR (PHA 8.6%-27.3%; P = 0.03, PWM 12.5%-26.6%; P = 0.07). In AIDS PHA cultures this did not change, and in their PWM cultures OKT 10 expression declined (44.8 to 23.0%; P = 0.05).(ABSTRACT TRUNCATED AT 400 WORDS)

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Human Interleukin 2: Physiology, Biochemistry, and Pathophysiology in Lymphoblastic Leukemias and Immunodeficiency Syndromes

Cited by 8 publications

References 17 publications

Impaired T- and B-cell functions in patients with Hodgkin's disease

Impaired T- and B-cell functions in patients with Hodgkin's disease

Einfluß von Human-Interleukin-2 auf die zelluläre Immunität bei Hämophilie A- und von Willebrand-Patienten im Kindesalter

T cell surface antigen expression on lymphocytes of patients with AIDS during in vitro mitogen stimulation

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