Human Infrapatellar Fat Pad-Derived Stromal Cells have more Potent Differentiation Capacity than other Mesenchymal Cells and can be Enhanced by Hyaluronan

Abstract

The microenvironment plays an important role in the homing in and differentiation of stem cells to repair injured tissue. Infrapatellar fat pad stromal cells (IFPSCs) are a promising source of such cells for the repair of articular injury-induced degeneration. This study investigated the chemotaxis of IFPSCs to chondrocytes and the effect of hyaluronan (HA) on the biological and regenerative properties of IFPSCs. The IFPSCs were obtained from patients undergoing arthroscopy and cultured via a standard 2-week culture protocol that yielded more than 10 million cells on passage 3. The results showed that the IFPSCs had a higher capacity for chondrogenic differentiation than mesenchymal cells from body fat, bone marrow, and Wharton's jelly of the umbilical cord. The IFPSCs cultured on 25% or 50% HA showed better osteogenic and adipogenic capabilities than those without HA or with 75% HA (p < 0.001). Cultures of the IFPSCs on 25% HA had a fourfold increase in chondrogenic differentiation compared to cultures without HA, which was better than with 50% and 75% HA (p < 0.05). Cell proliferation was not affected by the presence of HA. In conclusion, IFPSCs have a strong potential for chondrogenic regeneration, which can even be augmented in a 25% HA microenvironment.

Mesenchymal stromal cells from human umbilical cords display poor chondrogenic potential in scaffold-free three dimensional cultures

“…In our hands, the best combination for chondro-induction of HFPSCs and SMCSs were TGF-β3 and TGF-β1 respectively, in combination with BMP-2 and dexamethasone. These outcomes are also in line with previous studies showing chondrogenic differentiation of HFPSCs and SMSCs (Ding et al, 2015;Vinardell et al, 2012).…”

Human Umbilical Cord Mesenchymal Stem Cells: A New Era for Stem Cell Therapy

“…An unpublished study also reveals that HUCMSCs can effectively repair the monoiodoacetic acid (MIA)-injured cartilage via a decrease in the interleukin (IL)-1b levels induced by MIA treatment. Nevertheless, HUCMSCs possess chondrogenesis compared to stem cells derived from the infrapatellar fat pad (13).…”

Clinical Efficacy of Arthroscopic Therapy With Infrapatellar Fat Pad Cell Concentrates in Treating Knee Cartilage Lesion: a Randomized Controlled Trial

“…MSCs, such as bone marrow mesenchymal stem cells (BMSCs), synovial-derived mesenchymal stem cells (SDMSCs), and adipose-derived mesenchymal stem cells (ADMSCs), can be extracted from different kinds of tissue, whereas only MSCs derived from bone marrow and adipose can be acquired in a large amount for clinical use [14][15][16][26][27][28][29]. Compared with BMSCs,ADMSCs have more active proliferative capacity, and the acquiring procedure of them causes less damage to the donor zone [24,[30][31][32]. Meanwhile, studies have also showed that MSCs from different kinds of tissue have signi cantly different ability of proliferation and differentiation.…”