Human Infection with<i>Candidatus</i>Neoehrlichia mikurensis, China

Li, Hao; Jiang, Jia-Fu; Liu, Wei; Zheng, Yuan-Chun; Huo, Qiu-Bo; Tang, Kun; Zuo, Shuang-yan; Liu, Kun; Jiang, Bao-Gui; Yang, Hong; Cao, Wu‐Chun

doi:10.3201/eid1810.120594

Cited by 96 publications

(84 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Neoehrlichia mikurensis" based on groEL and 16S rRNA phylogenetic analyses of "Ca. Neoehrlichia mikurensis" DNA derived from rodents, ticks, and infected humans in China (14,18). A comparative alignment of the Chinese sequences with the deposited European "Ca.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe

et al. 2015

View full text Add to dashboard Cite

h "Candidatus Neoehrlichia mikurensis" is the tick-borne agent of neoehrlichiosis, an infectious disease that primarily affects immunocompromised patients. So far, the genetic variability of "Ca. Neoehrlichia" has been studied only by comparing 16S rRNA genes and groEL operon sequences. We describe the development and use of a multilocus sequence analysis (MLSA) protocol to characterize the genetic diversity of clinical "Ca. Neoehrlichia" strains in Europe and their relatedness to other species within the Anaplasmataceae family. Six genes were selected: ftsZ, clpB, gatB, lipA, groEL, and 16S rRNA. Each MLSA locus was amplified by real-time PCR, and the PCR products were sequenced. Phylogenetic trees of MLSA locus relatedness were constructed from aligned sequences. Blood samples from 12 patients with confirmed "Ca. Neoehrlichia" infection from Sweden (n ‫؍‬ 9), the Czech Republic (n ‫؍‬ 2), and Germany (n ‫؍‬ 1) were analyzed with the MLSA protocol. Three of the Swedish strains exhibited identical lipA sequences, while the lipA sequences of the strains from the other nine patients were identical to each other. One of the Czech strains had one differing nucleotide in the clpB sequence from the sequences of the other 11 strains. All 12 strains had identical sequences for the genes 16S rRNA, ftsZ, gatB, and groEL. According to the MLSA, among the Anaplasmataceae, "Ca. Neoehrlichia" is most closely related to Ehrlichia ruminantium, less so to Anaplasma phagocytophilum, and least to Wolbachia endosymbionts. To conclude, three sequence types of infectious "Ca. Neoehrlichia" were identified: one in the west of Sweden, one in the Czech Republic, and one spread throughout Europe.

show abstract

Section: Discussionmentioning

confidence: 99%

“…"Ca. Neoehrlichia mikurensis" infection may also pass unnoticed in healthy persons (13) or give rise to fever with additional symptoms (14).…”

mentioning

confidence: 99%

Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe

et al. 2015

View full text Add to dashboard Cite

show abstract

“…'Candidatus Neoehrlichia mikurensis' in an emerging tickborne zoonosis in Africa, Asia and Europe, and has a variety of non-specific clinical manifestations in humans including fever, headache and myalgia (von Loewenich et al, 2010;Li et al, 2012;Kamani et al, 2013). Rodents (Rattus norvegicus, Myodes spp.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic characterisation of two novel Anaplasmataceae from Australian Ixodes holocyclus ticks: ‘Candidatus Neoehrlichia australis' and ‘Candidatus Neoehrlichia arcana'

Gofton

Doggett

Ratchford

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

“…Neoehrlichia mikurensis" had been described in Europe (2), and 7 more were recently reported in China (14). Little is known about the distribution, risk areas, and zoonotic reservoir of this bacterium, and its pathogenic mechanisms remain largely uncharacterized.…”

mentioning

confidence: 99%

Asymptomatic “Candidatus Neoehrlichia mikurensis” Infections in Immunocompetent Humans

et al. 2014

View full text Add to dashboard Cite

bIn Europe, human infections with "Candidatus Neoehrlichia mikurensis" have mainly been restricted to immunocompromised patients. We report here the first cases of asymptomatic "Ca. Neoehrlichia mikurensis" infection in immunocompetent humans (5/316 [1.6%] were infected). Due to the potential threats of infections with "Ca. Neoehrlichia mikurensis" in healthy persons to the safety of the blood supply, further study of this phenomenon is required.

show abstract

Human Infection withCandidatusNeoehrlichia mikurensis, China

Cited by 96 publications

References 15 publications

Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe

Multilocus Sequence Analysis of Clinical “Candidatus Neoehrlichia mikurensis” Strains from Europe

Phylogenetic characterisation of two novel Anaplasmataceae from Australian Ixodes holocyclus ticks: ‘Candidatus Neoehrlichia australis' and ‘Candidatus Neoehrlichia arcana'

Asymptomatic “Candidatus Neoehrlichia mikurensis” Infections in Immunocompetent Humans

Contact Info

Product

Resources

About