Human immunoglobulin VH and VK repertoire revealed by in situ hybridization

Guigou, V.; Cuisinier, Anne-Marie; Tonnelle, Cécile; Moinier, Danielle; Fougereau, Michel; Fumoux, Francis

doi:10.1016/0161-5890(90)90161-r

Cited by 88 publications

(60 citation statements)

References 23 publications

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“…As has been noted in several studies using this and other techniques (18,29,30), the distribution of V H families in general reflects their germline complexity. Thus, V H 3, the family with the largest number of functional genes, was found most often.…”

Section: Discussionsupporting

confidence: 67%

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

Brezinschek¹,

Foster²,

Brezinschek³

et al. 1997

J. Clin. Invest.

326

291

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To analyze the immunoglobulin repertoire of human IgM ϩ B cells and the CD5ϩ and CD5 Ϫ subsets, individual CD19 ϩ / IgM ϩ /CD5 ϩ or CD5 Ϫ B cells were sorted and non-productive as well as productive V H gene rearrangements were amplified from genomic DNA and sequenced. In both subsets, the V H 3 family was overrepresented largely as a result of preferential usage of a small number of specific individual family members. In the CD5 ϩ B cell subset, all other V H families were found at a frequency expected from random usage, whereas in the CD5 Ϫ population, V H 4 appeared to be overrepresented in the nonproductive repertoire, and also negatively selected since it was found significantly less often in the productive compared to the nonproductive repertoire; the V H 1 family was significantly diminished in the productive rearrangements of CD5 Ϫ B cells. 3-23/DP-47 was the most frequently used V H gene segment and was found significantly more often than expected from random usage in productive rearrangements of both CD5

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Section: Discussionsupporting

confidence: 67%

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

Brezinschek¹,

Foster²,

Brezinschek³

et al. 1997

J. Clin. Invest.

326

291

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“…V family usage (VI-IV) in our Kde rearrangements was compared with V family usage in V-J rearrangements as reported in the literature and summarized in Table 4. 42,45,46,54,55 This comparison revealed that V family usage in V-J gene rearrangements and V-Kde rearrangements is comparable except for a higher tendency of VII family usage in V-Kde gene rearrangements of precursor-B-ALL. The most frequently used V gene segments of each V family found in V-J gene rearrangements are also frequently used in V-Kde gene rearrangements.…”

Section: Discussionmentioning

confidence: 93%

“…[42][43][44][45][46] But with the discovery of the PCR technique the analysis of antigen specific receptor gene rearrangements as well as the analysis of IGK gene rearrangements is moving rapidly. [47][48][49][50][51][52][53][54][55][56] However, reports about V gene usage in Kde rearrangements are not available.…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity in junctional regions of immunoglobulin kappa deleting element rearrangements in B cell leukemias: a new molecular target for detection of minimal residual disease

et al. 1997

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“…However, significantly higher frequency of V H 1 and lower frequency of V H 3 for VH were observed (Fig. 2), compared with those of reported antibody repertoires expressed in human peripheral blood B cells [20]. The yeast display constructs encoded N-terminal hemagglutinin (8 amino acids) and C-terminal c-myc (10 amino acids) tags flanking the scFv library for quantitative detection of surface-displayed scFvs [11,12].…”

Section: Construction Of An Anti-dr5 Pseudo-immune Scfv Librarymentioning

confidence: 85%

Construction and characterization of a pseudo-immune human antibody library using yeast surface display

Lee

Park

et al. 2006

Biochemical and Biophysical Research Communications

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Human immunoglobulin VH and VK repertoire revealed by in situ hybridization

Cited by 88 publications

References 23 publications

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5(+)/IgM+ and CD5(-)/IgM+ B cells.

Heterogeneity in junctional regions of immunoglobulin kappa deleting element rearrangements in B cell leukemias: a new molecular target for detection of minimal residual disease

Construction and characterization of a pseudo-immune human antibody library using yeast surface display

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