Human Immunodeficiency Virus Type 1 Replication Within Cystic Lymphoepithelial Lesion of the Salivary Gland

Abstract

Cystic lymphoepithelial lesions of salivary glands (CLLSG) are nodular or diffuse salivary gland enlargements that are observed in patients who tested positive for human immunodeficiency virus type 1 (HIV-1). Two cases of CLLSG are reported. Particular emphasis is placed on the presence of HIV-1 major-core protein (P24), HIV-1 RNA sequences, Epstein-Barr virus (EBV) DNA sequences, and lymphocyte receptor gene rearrangement. Lymphoid alterations consisted of explosive hyperplasia with a prominent follicular reticular dendritic cell (DRC) network and numerous intrafollicular CD8+ lymphocytes. Intrafollicular DRC strongly expressed HIV-1 major-core protein and HIV-1 RNA, indicating that most DRCs actively replicated the HIV-1 virus. The presence of active HIV-1 replication within DRC and the absence of clonal EBV infected lymphoid population strongly suggest that CLLSG pathogenesis is primarily induced by HIV-1. The presence of oligoclonal immunoglobulin gene rearrangements in our cases, however, suggest the need of long-term follow-up of such patients to determine whether CLLSG could be a benign prelymphomatous disease.

CD30-Positive Cutaneous Large Cell Lymphomas:A Comparative Study of Clinicopathologic and Molecular Features of 16 Cases

“…Tumor DNA was extracted from frozen material using a phenol chloroform method and Southern blot was performed as previously described. 19 Analysis of TCR7 chain gene rearrangement was performed using a multiplex PCR with GC-clamp primers followed by denaturing gradient gel electrophoresis (DGGE). Primers covering the entire 7 locus are used in a single reaction.…”

Lesión linfoepitelial quística benigna de glándula parótida en paciente con infección por VIH

“…Later and due to the increased incidence of HIV infection the number of such cases increased. 1,2 LBLC of the salivary glands affects the parotid more frequently than the sub maxillas [6][7][8] [6][7][8][9]13,23,25 Within…”

“…El componente linfoide tiene las mismas caracterís-ticas que se observan en las adenopatías de la infección por VIH menos avanzada, es decir, hiperplasia o fragmentación folicular, con agrandamiento de los centros germinales. 7,[9][10][11][12] El infiltrado linfoide está mayoritariamente formado por células B, aunque existen numerosos linfocitos T, principalmente CD89.…”

“…Suele aparecer en pacientes con síndrome poliadenopático persistente, con un núme-ro de linfocitos CD4 poco o medianamente disminuido y relativo aumento de células CD8 en sangre. [6][7][8][9]13,[16][17][18]23,25 Dentro de los estudios complementarios a realizar destacamos la TC, RM y ecografía; donde apreciaremos múltiples quistes parotídeos bilaterales asociados a adenopatías cervicales. Según distintos autores 11,12 las imágenes de TC y RM en pacientes VIH son patognomónicas, y no requerirían más estudios complementarios.…”

Découverte d’une infection à VIH à partir d’une sialomégalie bilatérale

“…L'épithélium est le siège d'une importante exocytose composée de lymphocytes, parfois retrouvés dans le liquide des cavités kystiques. L'HLK représente un important réservoir de VIH, confirmé par la présence d'une grande quantité de p24 et d'ARN viral, et d'une forte activité de réplication du VIH dans les cellules dendritiques des nodules lymphoïdes intercavitaires [10,11]. L'HLK est directement lié à l'effet cytopathogène du VIH.…”