Human Immunodeficiency Virus isolates from asymptomatic homosexual men and from AIDS patients have distinct biologic and genetic properties

Balachandran, Raghavan; Thampatty, Padma; Enrico, Amy; Rinaldo, Charles R.; Gupta, Phalguni

doi:10.1016/0042-6822(91)90027-9

Cited by 32 publications

(16 citation statements)

References 30 publications

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“…Unlike AIDS isolates, the asymptomatic isolates do not show productive infection in H9 cells and do not form syncytia in MT-2 cells. The asymptomatic isolates also replicate more slowly in phytohaemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC) and produce lower levels of viral DNA, RNA and proteins when compared to AIDS isolates (Balachandran et al, 1991). The results presented here support a mechanism of growth restriction of asymptomatic isolates in T cell lines at a step prior to provirus formation as previously described.…”

Section: Introductionsupporting

confidence: 71%

“…As reported earlier, all five AIDS isolates showed productive infection, producing > 1000 pg/ml of p24 within 6-8 days of infection; cells became chronically infected and produced virus (> 10000 pg/ml) continuously for at least 6 weeks (Balachandran et al, 1991). In contrast, none of the five asymptomatic isolates produced significant amounts of virus (< 100 pg/ml of p24) in H9 cells 12 days post-infection and also 21 days postinfection.…”

Section: Five Asymptomatic Isolates and Five Aids Isolatesmentioning

confidence: 72%

“…Infection of H9 cells was carried out as described (Balachandran et al, 1988(Balachandran et al, , 1991 except that the virus inoculum was incubated prior to infection with DNase I (80 U/ml) for 1 h at 37 °C in the presence of 10 mM-MgC12 and pelleted at 14000 g for 2 h at 4 °C in a microfuge (Levy & Shimabukuro, 1985). Virus pellets were resuspended in 1 ml of RPMI 1640 with 20 % serum and used as inoculum.…”

Section: Methodsmentioning

confidence: 99%

“…Virus isolates were propagated by infecting 1 x 107 PHA-stimulated PBMC with 1 x l0 s c.p.m, reverse transcriptase units (RTU)/ml as described (Balachandran et al, 1988(Balachandran et al, , 1991. Infection of H9 cells was carried out as described (Balachandran et al, 1988(Balachandran et al, , 1991 except that the virus inoculum was incubated prior to infection with DNase I (80 U/ml) for 1 h at 37 °C in the presence of 10 mM-MgC12 and pelleted at 14000 g for 2 h at 4 °C in a microfuge (Levy & Shimabukuro, 1985).…”

Section: Methodsmentioning

confidence: 99%

“…Analysis of biological characteristics of HIV-1 isolates from asymptomatic subjects and from AIDS patients (hereafter referred to as asymptomatic isolates and AIDS isolates, respectively) indicates distinct differences. We and others have shown that AIDS isolates are more virulent than most asymptomatic isolates with respect to their in vitro replication rates, ability to infect a wide variety of CD4 ÷ T cell lines and induction of syncytia (Balachandran et al, 1991 ;ChengMayer et al, 1988;Fenyo et al, 1988;Tersmette et al, 1989). Furthermore, transition from non-syncytiuminducing to syncytium-inducing HIV-1 isolates in infected patients has been shown to be associated with the development of AIDS (Koot et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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An additional mechanism of growth restriction in T cell line H9 of human immunodeficiency virus type 1 isolates from asymptomatic homosexual men

Balachandran

Singh²,

Gupta³

1996

Journal of General Virology

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The replicative properties of human immunodeficiency virus type 1 (HIV-1) isolates from asymptomatic carriers (asymptomatic isolates) and AIDS patients (AIDS isolates) were examined in the human T lymphocyte cell line H9. In agreement with earlier reports the replication of asymptomatic isolates was restricted whereas AIDS isolates replicate well in H9 cells. PCR analysis of H9 cells infected with asymptomatic isolates showed transient gag DNA synthesis for up to 48 h post-infection. suggesting an additional restriction mechanism for asymptomatic isolates in H9 cell lines at a step post-DNA synthesis and prior to or during nuclear translocation of newly synthesized viral DNA.

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Section: Introductionsupporting

confidence: 71%

Section: Five Asymptomatic Isolates and Five Aids Isolatesmentioning

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An additional mechanism of growth restriction in T cell line H9 of human immunodeficiency virus type 1 isolates from asymptomatic homosexual men

Balachandran

Singh²,

Gupta³

1996

Journal of General Virology

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Efficient and reproducible new semimicromethod for the detection and titration of HIV in human plasma

Andreoni

Sarmati

Parisi

et al. 1992

Journal of Medical Virology

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A semimicromethod was established for isolating human immunodeficiency virus (HIV) in plasma using 48-well plates and a pool of peripheral blood mononuclear cells (PBMC) from several donors as targets for infection, which increases the efficiency of isolation by reducing the effect of variability due to diverse donor cell susceptibility to HIV infection. The addition of H9 cells to the PBMC cultures did not affect measurable titers. Nevertheless, it potentiated strongly virus replication in terms of p24 production in the supernatant of the wells with HIV isolates, thus facilitating interpretation of the results. The titration of a virus strain of a known titre and reverse transcriptase activity in parallel provided a constant parameter of efficiency and reproducibility within each experiment, permitting comparison with results from other laboratories. The reproducibility of the method was highly significant (r = 0.97, P < 0.001); 68% of the 22 plasma samples from HIV-infected individuals tested by this method were positive. The presence of plasma HIV titer correlated well (P < 0.02) with the low count of CD4+ cells of less than 300/mm3, but not with the presence of the p24 antigen in the serum.

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Immunopathogenesis of AIDS

Zijenah

Katzenstein

AIDS in Africa

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Human Immunodeficiency Virus isolates from asymptomatic homosexual men and from AIDS patients have distinct biologic and genetic properties

Cited by 32 publications

References 30 publications

An additional mechanism of growth restriction in T cell line H9 of human immunodeficiency virus type 1 isolates from asymptomatic homosexual men

An additional mechanism of growth restriction in T cell line H9 of human immunodeficiency virus type 1 isolates from asymptomatic homosexual men

Efficient and reproducible new semimicromethod for the detection and titration of HIV in human plasma

Immunopathogenesis of AIDS

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