Human Immunodeficiency Virus Infection in Cells of Myeloid‐Monocytic Lineage

Ushijima, Hiroshi; Dairaku, Masatake; Honma, Hitoshi; Yamaguchi, Koushi; Shimizu, Hiroyuki; Tsuchie, Hideaki; Abe, Kenji; Yamamoto, Akiko; Hoshino, Hiroo; Müller, Wernér E.G.

doi:10.1111/j.1348-0421.1991.tb01579.x

Cited by 9 publications

(12 citation statements)

References 22 publications

(11 reference statements)

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“…[33][34][35][36][37] Here, we identified additional cell lines (PB-1, Sez-4, HUT102B, ATL-2, C91PL, L540, C10MJ2 and Karpas 299) which should also be useful tools for studying various aspects of HIV biology. The possibility that human hematopoietic cell lines could become infected by HIV should always be kept in mind, especially in those laboratories that have broad access to human blood products.…”

Section: Discussionmentioning

confidence: 99%

Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines

et al. 2000

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The aim of this study was to learn more about the role of the HIV-related chemokine-chemokine receptor axes in human hematopoiesis. To address this issue we phenotyped 35 selected hematopoietic cell lines for the expression of CD4, CXCR4 and CCR5. We next evaluated the functionality of these chemokine receptors by calcium flux and chemotaxis assays, and by the ability of SDF-1, MIP-1␣, MIP-1␤ and RANTES to influence the growth of the cells expressing CXCR4 and/or CCR5. Lastly, we examined whether human hematopoietic cell lines may secrete some HIV-related chemokines, and whether endogenously secreted chemokines might interfere with the infectability of hematopoietic cells by X4 and R5 HIV strains. These results demonstrate that: (1) HIV-related receptors are widely expressed on human hematopoietic cell lines; (2) stimulation of CXCR4 by SDF-1 induces calcium flux and chemotaxis in several hematopoietic cell lines more efficiently than stimulation of CCR5 by receptor-specific ␤-chemokines; (3) chemokines do not regulate proliferation of the hematopoietic cells; and finally (4) infectability of the hematopoietic cells by HIV-1 may be auto-modulated by endogenously secreted chemokines. These data shed more light on the role of HIV-related chemokine-chemokine receptors axes in human hematopoiesis and interaction of hematopoietic cells with HIV. Leukemia (2000) 14, 1821-1832.

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Section: Discussionmentioning

confidence: 99%

Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines

et al. 2000

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“…This is because of their ability to be infected in an unactivated state as well as because of the development of the OM-10.1 cell, a clonally derived cell line from HIV-1-infected HL-60 promyelocytes which harbor a single integrated provirus that is silent until activated [138]. In the beginning of the epidemic, a number of studies were conducted with the HL-60 and OM-10.1 cells to determine and characterize the viral infection and replication dynamics within cells of this lineage [138][139][140][141][142][143]. These cells have been shown to retain CD4, CXCR4, and CCR5 expression and retain CD4 expression unless viral replication is active [138].…”

Section: Hl-60mentioning

confidence: 99%

Myelomonocytic Cell Lines in Modeling HIV-1 Infection of the Bone Marrow

Nonnemacher¹,

Quiterio²,

Allen³

et al. 2017

Biology of Myelomonocytic Cells

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Human immunodeficiency virus type 1 (HIV-1), the etiologic agent of acquired immunodeficiency syndrome (AIDS), primarily infects T cells and cells of the monocyte-macrophage lineage. This is due to the presence of the cell surface receptor CD4 and the coreceptors, CXCR4, and CCR5. While the T-cell has classically been the cell type associated with HIV-1 disease progression, cells of the monocyte-macrophage lineage have also been shown to play a major role in this viral pathologic process. Classically, this has involved monocytic cells in the peripheral blood and tissue macrophages, however, over the course of HIV disease, the promyelomonocytic cells of the bone marrow (BM) have also been shown to play a role in pathogenesis retroviral disease in that they play an integral role in the reseeding of the periphery and end-organ tissues. This has involved an initial infection of the bone marrow hematopoietic progenitor cells. Given this observation, over the years there have been a number of cell lines that have been developed and provided valuable insights into research questions surrounding HIV-1 infection of the monocyte-macrophage cell lineage. In this regard, we will examine the biological and immunological properties of these BM-derived cell lines with respect to their utility in exploring the pathogenesis of HIV-1 in humans.

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“…THP-I, U-937). [2] as well; persistent infection was achieved even after a period of more than 2 years. The reinfection abilities of the virus isolates differ.…”

Section: Discussionmentioning

confidence: 83%

“…and the promonocyticcel! lines THP-1 (CD4 anti gen positive) as well as U-937 [2], After an acute infection, the number of HIV-antigen-producing promyelomonocytic cells of the lines ML-1 and HL-60 increased slowly compared to the promonocytic cell lines THP-1 and U-937 [2]. IIIB).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Cells of the Myeloid-Monocytic Lineage (ML-1, HL-60, THP-1, U-937) Chronically Infected with the Human Immunodeficiency Virus-1

Ushijima

Ami²,

Tsuchie³

et al. 1993

Pathobiology

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The myeloid-monocytic cells ML-1, HL-60, THP-1 and U-937 were chronically infected (for more than 2 years) with the lymphotropic HIV-1 strain HTLV-IIIB. Reinfection experiments revealed that viruses obtained from chronically infected ML-1 /HIV-1 and HL-60/HIV-1 cells show a low infectivity if tested with uninfected ML-1 and HL-60 cells in contrast to virus preparations from chronically infected THP-l/HIV-1 and U-937/HIV-1 with their corresponding uninfected cell lines. Analyses of selected cell surface markers showed a differential expression of CD4, CD8, CD1 lc, CD 14, CD 15, CD20, HLA-DR and HLA-DQ in non- or chronically infected cells. During chronical infection, the myeloid-monocytic cells lost their reactivity with peroxidase and esterase. In chronically infected cells, the steady-state levels for TNF-α mRNA remained unchanged while those for IL-6 decreased. The half-lives of transcripts of both TNF-α (t½: 70 min) and IL-6 (t½: 100 min) were nearly the same in uninfected and chronically infected HL-60 cells.

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Human Immunodeficiency Virus Infection in Cells of Myeloid‐Monocytic Lineage

Cited by 9 publications

References 22 publications

Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines

Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines

Myelomonocytic Cell Lines in Modeling HIV-1 Infection of the Bone Marrow

Characterization of Cells of the Myeloid-Monocytic Lineage (ML-1, HL-60, THP-1, U-937) Chronically Infected with the Human Immunodeficiency Virus-1

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