Human IgG subclass antibodies to the 19 kilodalton carboxy terminal fragment of &lt;i&gt;Plasmodium Falciparum&lt;/i&gt; merozoite surface protein 1 (MSP1&lt;sub&gt;19&lt;/sub&gt;) and predominance of the MAD20 allelic type of MSP1 in Uganda

Apio, Baru Ruth Sharon; Nalunkuma, A; Okello, Denis; Riley, E. M.; Tg, Egwang

doi:10.4314/eamj.v77i4.46620

Cited by 10 publications

(10 citation statements)

References 20 publications

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“…This was slightly lower than the previously reported parasite prevalence of 70-90% in the same age group living in neighboring parishes in Apac District in 1995-1999 [17][18][19]31 but within the 50-100% range reported in world malaria maps. 32 When PCR was used for parasite detection, overall P. falciparum parasite prevalence was 55.2% in all age groups (37.5% by microscopy) and 69.3% in children 2-9 years of age (57.3% by microscopy).…”

Section: Discussioncontrasting

confidence: 60%

“…According to surveys conducted in 2001-2002, this area experiences perennial holoendemic malaria 17 with parasite prevalence rates of 70-90% in children < 10 years of age. [17][18][19] The entomological inoculation rate was estimated at > 1,500 infective bites per person per year and the major vector responsible for transmission is Anopheles funestus . 17 Plasmodium falciparum is the dominant parasite species, Plasmodium malariae being responsible for ~3% of the infections and Plasmodium ovale was previously not observed.…”

Section: Methodsmentioning

confidence: 99%

“…15,16 In this study, we determined the current level of malaria transmission intensity in the Apac district in northern Uganda, a remote region that was previously described as holoendemic for malaria. [17][18][19] Northern Uganda has been involved in conflict since the early 1980s with the Lord's Resistance Army as the main rebel group that continues to be a threat to the region up to the present day. This conflict hindered economic development in northern Uganda and resulted in a lower access to healthcare compared with other regions in Uganda.…”

Section: Introductionmentioning

confidence: 99%

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Continuing Intense Malaria Transmission in Northern Uganda

Proietti¹,

Pettinato²,

Kanoi³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Abstract. Recent reports of reductions in malaria transmission in several African countries have resulted in optimism that malaria can be eliminated in parts of Africa where it is currently endemic. It is not known whether these trends are global or whether they are also present in areas where political instability has hindered effective malaria control. We determined malaria parasite carriage and age-dependent antibody responses to Plasmodium falciparum antigens in cross-sectional surveys in Apac, northern Uganda that was affected by political unrest. Under-five parasite prevalence was 55.8% (115/206) by microscopy and 71.9% (41/57) by polymerase chain reaction. Plasmodium ovale alone, or as a co-infection, was detected in 8.6% (12/139) and Plasmodium malariae in 4.3% (6/139) of the infections. Age seroprevalence curves gave no indication of recent changes in malaria transmission intensity. Malaria control remains a tremendous challenge in areas that have not benefited from large-scale interventions, illustrated here by the district of Apac.

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Section: Discussioncontrasting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Continuing Intense Malaria Transmission in Northern Uganda

Proietti¹,

Pettinato²,

Kanoi³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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“…[13,19] In addition, MSP-1 and MSP-2 genes could elicit immune response in humans and have been identified as potential candidates for blood-stage malarial vaccine. [13,20,21]…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in MSP-1 gene of Plasmodium falciparum and its association with malaria severity, parasite density, and host factors of asymptomatic and symptomatic patients in Papua, Indonesia

Sorontou¹,

Pakpahan²

2015

Int J Med Sci Public Health

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Background: Genetic diversity of Plasmodium falciparum represents a major issue in understanding the several aspects of malaria infection and diseases. The merozoite surface protein (MSP)-1 gene of P. falciparum codes for a major asexual blood-stage antigen, presently proposed as a major malarial vaccine candidate. Objective: To determine the length of the DNA bands and genetic diversity of the MSP-1 gene of P. falciparum, which is associated with malaria severity and host factors in population of the asymptomatic and symptomatic patients in Papua, Indonesia. Materials and Methods: A total of 152 isolates from the blood samples of the patients infected with P. falciparum were analyzed by microscopic examination. DNA was extracted from MSP-1 gene and nested PCR amplification carried out. The active case detection was conducted in Koya and Skow villages, whereas passive detection was obtained from general hospitals. Result: The main allele of the gene MSP-1, which related to the severity of malaria, the parasite density, and host factors of the population with a single infection, was found to MAD20 that was higher in mild malarial cases. The family alleles K1, MAD20, and RO33 of the MSP-1 gene of P. falciparum were found to be in large numbers in severe malaria and light malaria of the symptomatic patients. In the asymptomatic patients, it was found that the alleles of MSP-1 (K1, MAD20, and RO33) were more numerous when the parasite density was less than 10,000/μL of blood. Conclusion: There was no significant correlation between the genetic diversity of the MSP-1 gene of P. falciparum with malaria severity, parasite density, age group, and gender of asymptomatic patients, but there was a significant correlation with the age groups and gender in the symptomatic patients. The K1, MAD20, and RO33 alleles showed a higher density in asymptomatic patients, but K1 and RO33 alleles were higher at severe and mild malaria and MAD20 was higher at mild malaria cases. Therefore, MSP-1 gene allele could be used as a genetic marker to determine the genetic diversity of P. falciparum and as a candidate molecular vaccine.

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“…During the blood stage, the parasite expresses arrays of proteins and among others are merozoite surface protein 1 (MSP 1) and MSP 2. These proteins are involved in erythrocyte invasion [3] and are targeted by the immune responses [4,5], and therefore have been used as target for vaccine development. MSP 1 and MSP 2 also exhibit high polymorphisms hence play important role in identi cation of genetically distinct P. falciparum parasite sub-populations [3].…”

mentioning

confidence: 99%

Allelic diversity of merozoite surface protein-1 (MSP 1) and MSP 2 genes, and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia

Jamil

Pratama

Marantina

et al. 2020

Preprint

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Background Malaria control programme in Indonesia has successfully brought down malaria incidence in many parts of the country, including Aceh Province. Clinical manifestation of reported malaria cases in Aceh varied widely from asymptomatic, mild uncomplicated to severe and fatal complications. The present study aims to explore the allelic diversity of merozoite surface protein genes among the P. falciparum isolates in Aceh Province and to determine their potential correlation with the severity of malaria clinical manifestation. Methods Screening of over 500 malaria cases admitted to the 11 districts hospital within Aceh Province during 2013–2015, identified 90 cases of P. falciparum mono-infection without any co-morbidity. The subjects were clinically phenotyped and parasite DNA was extracted and Polymerase Chain Reaction (PCR) amplified for the MSP1 and MSP2 allelic subfamilies. Results Analysis of clinical manifestation revealed that fever-chill is the most frequent symptom. Based on WHO criteria showed 19 cases classified as severe and 71 as mild malaria. Analysis of MSP 1 gene revealed the presence of K1 allele subfamily in 34 subjects, MAD20 in 42 subjects, RO33 in 1 subject, and mixed allelic of K1 + MAD20 in 5 subjects, K1 + RO33 in 4 subjects, and MAD20 + RO33 in 4 subjects. Analysis of MSP 2 gene revealed 34 subjects carried the FC27 allelic subfamily, 37 subjects (carried the 3D7 and 19 subjects carried the mixed FC27 + 3D7. Analysis of multiplicity of infection revealed that msp2 alleles is slightly higher than MSP 1 with the mean of MOI were 2.69 and 2.27, respectively. Statistical analysis to determine the association between each clinical manifestation and MSP 1 and MSP 2 alleles revealed that liver function abnormal value was associated with the MSP 2 mixed alleles (p < 0.05; odds ratio = 0.13; CI95%= 0.03–0.53). Malaria severity was associated with the mixed MSP 1 of K1 + RO33 with severe malaria (p < 0.05; odds ratio = 28.50; CI 95%= 1.59-1532.3). Conclusion This study found a strong association between severe malaria in Aceh with subjects carrying the MSP 1 mixed alleles of K1 and RO33. The liver function abnormal value associated with the MSP 2 mixed allelic subfamilies. Further study in different geographic areas is recommended.

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Human IgG subclass antibodies to the 19 kilodalton carboxy terminal fragment of <i>Plasmodium Falciparum</i> merozoite surface protein 1 (MSP1<sub>19</sub>) and predominance of the MAD20 allelic type of MSP1 in Uganda

Cited by 10 publications

References 20 publications

Continuing Intense Malaria Transmission in Northern Uganda

Continuing Intense Malaria Transmission in Northern Uganda

Genetic diversity in MSP-1 gene of Plasmodium falciparum and its association with malaria severity, parasite density, and host factors of asymptomatic and symptomatic patients in Papua, Indonesia

Allelic diversity of merozoite surface protein-1 (MSP 1) and MSP 2 genes, and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia

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Human IgG subclass antibodies to the 19 kilodalton carboxy terminal fragment of <i>Plasmodium Falciparum</i> merozoite surface protein 1 (MSP1<sub>19</sub>) and predominance of the MAD20 allelic type of MSP1 in Uganda

Cited by 10 publications

References 20 publications

Continuing Intense Malaria Transmission in Northern Uganda

Continuing Intense Malaria Transmission in Northern Uganda

Genetic diversity in MSP-1 gene of Plasmodium falciparum and its association with malaria severity, parasite density, and host factors of asymptomatic and symptomatic patients in Papua, Indonesia

Allelic diversity of merozoite surface protein-1&nbsp;(MSP 1) and MSP 2 genes, and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia

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Product

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Allelic diversity of merozoite surface protein-1 (MSP 1) and MSP 2 genes, and clinical manifestations of Plasmodium falciparum malaria cases in Aceh, Indonesia