Human IgG and IgA subclass response following immunization with a polyvalent Klebsiella capsular polysaccharide vaccine

Abstract

The human IgG and IgA response was determined after vaccination with an experimental 24-valent Klebsiella capsular polysaccharide vaccine. The majority of natural antibody acquired prior to immunization was found in the IgG1, IgG2 and IgA1 subclasses. The immune response to vaccination was concentrated within the IgG1, IgG2, IgA1 and IgA2 subclasses with greater than or equal to 70% of subjects responding with a significant (greater than or equal to fourfold) rise in titer. The IgG3 and IgG4 response was meager with few volunteers (0%-40%) showing a significant titer rise. Therefore, vaccination with Klebsiella capsular polysaccharide induces a vigorous IgG and IgA response in humans which is not restricted to single antibody class or subclass.

A murine model demonstrates capsule-independent adaptive immune protection in survivors of Klebsiella pneumoniae respiratory tract infection

“…Instead, experiments have assessed adaptive immunity elicited by heat-killed organisms and a variety of specific K. pneumoniae immunogenic factors, including outer membrane vesicles, O-antigens, type 3 fimbriae and purified capsule (Chen et al, 2011;Cryz et al, 1985;Lee et al, 2015;Amezcua Vesely et al, 2019;Lavender et al, 2005;Trautmann et al, 2004;Hegerle et al, 2018). Early work in rodents and subsequently humans indicated that immunization with capsule elicits serotype-specific antibody responses (Cryz et al, 1985;Alcantar-Curiel et al, 1993;Cryz et al, 1988Cryz et al, , 1984Cryz et al, , 1986a; however, capsule is not the sole driver of protective immunity during infection (Lee et al, 2015;Lundberg et al, 2013). Moreover, it is currently unknown whether invasive infection in patients elicits durable protective immunity.…”

Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains

“…Persistence of anti-PIA and anti-Gly-TA antibodies in the immunized mice sera was achieved by applying commercial enzyme-linked immunosorbent assay (ELISA) kit (Sino Biologic, Inc,) conforming to the manufacturer instructions by coating the 1 μg/well of PIA and an appropriate dilution of capture goat anti-mouse IgG antibody overnight at 4 °C. Then the procedure was done based on the previous published [16]. The absorbance at 492 nm was measured and the amount of antibodies was predicted by comparing the control group based on the optical density.…”

The Promising Potential of Reverse Vaccinology-Based Next-Generation Vaccine Development over Conventional Vaccines against Antibiotic-Resistant Bacteria

“…Bacterial capsule-targeted vaccines might serve as an effective strategy to immunize against encapsulated pathogens, as evidenced by the success of capsule-targeted vaccines against Streptococcus pneumoniae , Neisseria meningitides , and Haemophilus influenza [ 181 , 182 ]. A monovalent K. pneumoniae K1 capsule polysaccharide (CPS) vaccine was developed in 1985 and several polyvalent K. pneumoniae CPS vaccines were developed in 1986, but these vaccines were associated with the elicitation of only T cell responses without the elicitation of high affinity neutralizing antibodies [ 183 , 184 , 185 , 186 ]. (B cell responses are classified as T-dependent (T-D) or T-independent (T-I), based on the requirement for T cell help in antibody production.)…”