Human Herpesvirus 8 Is Present in the Lymphoid System of Healthy Persons and Can Reactivate in the Course of AIDS

Bigoni, Beatrice; DoIcetti, R.; Lellis, Laura De; Carbone, Antonino; Boiocchi, Mauro; Cassai, Enzo; Luca, Dario Di

doi:10.1093/infdis/173.3.542

Cited by 146 publications

(74 citation statements)

References 20 publications

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“…Although it was used with samples from a limited number of patients, the sensitivity of this biological assay was found to be significantly higher than those of molecular techniques, such as nested PCR with freshly isolated PBMCs or stimulated PBMCs. Indeed, in our experiments, the nested PCR analyses identified sequences in only 4 and 9% of the patients, respectively, detection rates similar to those observed in the Italian general population (3).…”

Section: Discussionsupporting

confidence: 87%

Use of a BJAB-Derived Cell Line for Isolation of Human Herpesvirus 8

et al. 2005

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Establishment of latently infected cell lines from primary effusion lymphomas (PEL) presently is the most efficient system for the propagation of clinical strains of human herpesvirus 8 (HHV-8) in culture. Here we describe a new approach to culture productively replicating HHV-8 from patient samples. A BJAB-derived B-cell line, BBF, was found to retain HHV-8 longer, to support the latent and lytic replication programs, and to produce transmissible virus. Supernatants from n-butyrate-treated peripheral blood mononuclear cells of 24 HHV-8-seropositive renal transplant recipients were used to infect BBF cells, and replicating virus was detected in cultures from 11 patients. Moreover, BBF cells infected with saliva strains showed a highly productive profile regardless of the initial viral load, which confirms that infectious HHV-8 can be present in saliva and also suggests that saliva strains may exhibit a high tropism for B lymphocytes. In conclusion, we established an in vitro system that efficiently detects HHV-8 in samples with low viral loads and that produces infectious progeny. BBF cells can be used to propagate HHV-8 from different biological samples as well as to clarify important issues related to virus-cell interactions in a context distinct from endothelial and PELderived cell lines.

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Section: Discussionsupporting

confidence: 87%

Use of a BJAB-Derived Cell Line for Isolation of Human Herpesvirus 8

et al. 2005

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“…The viral load is significantly higher in lymphoid tissue from HIV-infected persons as compared to HIV-seronegative individuals 85 , although it is still lower than in splenic tissue or PBMCs from the same patients. This suggests that the HHV-8 detected in these lesions may reflect HHV-8 carriage by non-neoplastic B cells 59 .…”

Section: Other Possible Disease Associations Of Hhv-8mentioning

confidence: 75%

“…HHV-8 DNA has also been detected rarely in other lymphoproliferative disorders, including nonHodgkin's lymphoma, Hodgkin's disease, reactive lymphadenopathies 85 , and cutaneous lymphoma in AIDS 59 . The viral load is significantly higher in lymphoid tissue from HIV-infected persons as compared to HIV-seronegative individuals 85 , although it is still lower than in splenic tissue or PBMCs from the same patients.…”

Section: Other Possible Disease Associations Of Hhv-8mentioning

confidence: 99%

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Leão¹,

Caterino–de–Araujo

Porter

et al. 2002

Rev. Hosp. Clin.

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OBJECTIVE: To review the current literature on human herpesvirus 8 with particular attention to the aspects related to the etiopathogenesis of Kaposi's sarcoma. MATERIALS AND METHODS: The authors searched original research and review articles on specific aspects of human herpesvirus 8 infection, including virology, epidemiology, transmission, diagnosis, natural history, therapy, and Kaposi's sarcoma etiopathogenesis. The relevant material was evaluated and reviewed. RESULTS: Human herpesvirus 8 is a recently discovered DNA virus that is present throughout the world but with major geographic variation. In the Western world, the virus, transmitted mainly by means of sexual contact, is strongly associated with Kaposi's sarcoma and body cavity-based lymphoma and more controversially with multiple myeloma and other non-proliferative disorders. There is no specific effective treatment, but HIV protease inhibitors may play an indirect role in the clearance of human herpesvirus 8 DNA from peripheral blood mononuclear cells of HIV-infected patients. Human herpesvirus 8 DNA is present in saliva, but there are as yet no documented cases of nosocomial transmission to health care workers. The prevalence of human herpesvirus 8 among health care workers is probably similar to that in the general population. CONCLUSION: Human herpesvirus 8 appears to be, at least in Western Europe and United States, restricted to a population at risk of developing Kaposi's sarcoma. Human herpesvirus 8 certainly has the means to overcome cellular control and immune responses and thus predispose carriers to malignancy, particularly Kaposi's sarcoma. The wide diffusion of Human herpesvirus 8 in classic Kaposi's sarcoma areas appears to represent an important factor in the high incidence of the disease. However, additional co-factors are likely to play a role in the development of Kaposi's sarcoma.

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“…42 Studies to detect HHV-8 in PBMCs by PCR have yielded variable results ranging from 0-9% of normal donors in Europe and the US (with low rates being observed in the UK). [43][44][45] Given that seven out of the 20 in this initial panel of cALL samples tested positive for one of these human herpesviruses, we extended the study in order to both quantify the level of positivity detected and to determine whether control children show similar levels of positivity.…”

Section: Conventional Analysesmentioning

confidence: 99%

Screening for herpesvirus genomes in common acute lymphoblastic leukemia

et al. 2001

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There is epidemiological evidence that infection may play a role in the etiology of childhood leukemia in particular common B cell precursor acute lymphoblastic leukemia. A panel of 20 leukemic samples (panel 1) was examined for the presence of four lymphotropic herpesviruses using conventional molecular techniques. A second independent panel of 27 leukemic samples (panel 2), along with 28 control peripheral blood samples from children with other forms of cancer, was tested for the presence of the same four viruses using sensitive realtime quantitative PCR. While herpesvirus genomes were detected, they were present at very low levels; detection rates and levels were similar in the leukemic and control panels. In addition we surveyed 18 leukemic samples (five from panel 1, six from panel 2 and a further seven samples not previously analyzed) using a degenerate PCR assay capable of detecting the genomes of known herpesviruses plus putative new members of the family. No novel herpesvirus genomes were detected suggesting that a herpesvirus is unlikely to be etiologically involved as a transforming agent in common acute lymphoblastic leukemia. Leukemia (2001) 15, 415-421.

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Human Herpesvirus 8 Is Present in the Lymphoid System of Healthy Persons and Can Reactivate in the Course of AIDS

Cited by 146 publications

References 20 publications

Use of a BJAB-Derived Cell Line for Isolation of Human Herpesvirus 8

Use of a BJAB-Derived Cell Line for Isolation of Human Herpesvirus 8

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Screening for herpesvirus genomes in common acute lymphoblastic leukemia

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