Human herpesvirus 6 in monocytes of transplant patients

Wrzos, Helena; Gibbons, Joseph M.; Abt, PeterL.; Gifford, RobertR.M.; Yang, HaroldC.

doi:10.1016/0140-6736(90)90729-o

Cited by 17 publications

(4 citation statements)

References 5 publications

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“…As HHV-6 has a tropism similar to that of human immunodeficiency virus (HIV) in infections of CD4 ÷ T lymphocytes and monocytes/macrophages (Yamanishi et al, 1988;Okuno et al, 1989;Levy et al, 1990a;Kondo et al, 1991;Wrzos et al, 1990), it has been suggested that HHV-6 may reactivate and participate in AIDS or cause complications in immunodeficient transplant or cancer patients (Lusso et al, , 1991Morris et al, 1989;Ward et al, 1989;Okuno et al, 1990;Carrigan et al, 1991). Complications include fatal fulminant hepatitis (Asano et al, 1990) and HHV-6-associated interstitial pneumonitis in bone marrow transplant (BMT) patients .…”

Section: Introductionmentioning

confidence: 99%

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Gompels

Carrigan

Carss

et al. 1993

Journal of General Virology

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Fifteen human herpesvirus 6 (HHV-6) strain variants were analysed by PCR amplifications, restriction enzyme site polymorphism and sequence analyses. Three DNA regions were chosen for study: a fragment of a variable glycoprotein gene (210 bp), the conserved glycoprotein H (gH) gene complete with intergenic sequences (2381 bp) and the 5' intergenic region with the Nterminal coding sequence of gH up to a polymorphic BamHI site (427 bp). Infected cell DNA from five laboratory reference strains including GS, Ul102, AJ, Z29 and KF were examined together with DNA from peripheral blood lymphocytes infected with HHV-6 reactivated from blood and/or marrow from five bone marrow transplant (BMT) patients. Separate blood and marrow isolates were obtained from four BMT patients. In addition, HHV-6 sequences were examined directly from one of six Hodgkin's lymphomas and six B cell proliferations which contained HHV-6 DNA as detected by PCR amplification. The results show two groups of very closely related but heterogeneous strains which correlate with previous groupings by antigenic and restriction site differences. These are variant A strains (including laboratory strains GS, Ul102 and AJ) and variant B strains (including laboratory strains Z29 and KF, the Hodgkin's lymphoma strain, and the nine BMT patient isolates). Variations between the groups were 4 to 6 % in nucleotide sequence and 5 to 8.5 % in amino acid sequence. Within each group maximum heterogeneity was observed in different genes. Variant A strains differed by 2-0% in the variable glycoprotein gene sequence whereas variant B strains were identical in this region; conversely, variant B strains differed by 2 to 3 % in the gH N-terminal and intergenic sequences whereas variant A strains differed there by less than 0'2%. There was evidence for sequence drift independent of selection: relationships between the groups were shown by analyses of amino acid sequence, coding nucleotide sequence as well as intergenic sequence, and the B variant-specific BamHI site in the gH gene was due to a non-coding nucleotide substitution. There was little evidence for in vivo or in vitro variation: the gH nucleotide sequence from the uncultured lymphoma strain (first variant B gH gene identified) was almost identical to the gH sequence from four BMT isolates, and matched BMT isolates from blood and marrow were identical or with a single nucleotide substitution. The overall variation observed between the HHV-6 strain groups was similar or less than that seen between human cytomegalovirus strains such as AD169 or Towne, but deafly distinct from the much greater divergence between currently designated herpesvirus species.

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Section: Introductionmentioning

confidence: 99%

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Gompels

Carrigan

Carss

et al. 1993

Journal of General Virology

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“…Human herpesvirus-6 (HHV-6) is a recently recognized human herpesvirus, considered to be the causative agent for exanthem subitum (roseola infantum) [Yamanishi et a1.,1988. Thisvirus, initially termed human B-lymphotropic virus, was originally isolated from blood of a n immunosuppressed host with the antibody [Salahuddin et al, 19861 and later from the seropositive organ transplant recipients [Ward et al, 1989;Wrzos et al, 1990;Okuno et al, 1990;Yoshikawa et al, 19911. It is generally believed that HHV-6 is rarely isolated from blood of adults but persists in the host after recovery from exanthem subitum with persistent or intermittent shedding of active virus from the oropharynx [Harnett et al, 1990;Levy et al, 19901.…”

Section: Introductionmentioning

confidence: 99%

Activation of human herpesvirus‐6 in children with acute measles

Suga

Yoshikawa

Asano

et al. 1992

Journal of Medical Virology

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Virological and serological studies were carried out prospectively to evaluate the possible activation of human herpesvirus-6 (HHV-6) in 50 infants and children with acute measles by isolation of HHV-6 from peripheral blood and by determining neutralizing antibodies to the virus. All but 5 patients (90%) were seropositive to HHV-6 in the acute stage of measles and 18 (40%) had a significant increase in HHV-6 antibody titers thereafter, whereas only 2 of 27 patients who were initially seropositive to Epstein-Barr virus (EBV) viral capsid antigen (VCA) had a significant rise in antibody titers to EBV VCA. Among 18 patients with a significant increase in HHV-6 titers, the virus was isolated from the peripheral blood mononuclear cells of three patients in the early convalescent stage of measles. These results indicate that activation of HHV-6 may occur frequently a few weeks after primary infection with the measles virus.

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“…Josephs et al (31) and Jarrett et al (30) demonstrated the presence of HHV-6 DNA sequences in some lymphoma tissue, although these appeared to be relatively isolated cases. The highly cytopathic effect of HHV-6 in tissue culture, coupled with the ability of the virus to reactivate in transplant cases (4,59,61), may point to important clinical implications for immunosuppressed individuals in general. In vitro experiments show that HHV-6 can transactivate the human immunodeficiency virus long terminal repeat linked to a reporter gene, enhancing HIV gene expression and CD4+ cell death in cultures coinfected with both viruses (1,18,26,40).…”

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confidence: 99%

Characterization of the DNA polymerase gene of human herpesvirus 6

Teo¹,

Griffin²,

Jones³

1991

J Virol

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The construction of a recombinant bacteriophage A library containing overlapping clones covering 155 kbp of the 161-kbp genome of the Ugandan U1102 isolate of human herpesvirus 6 (HHV-6) is described. The use of degenerate-primer polymerase chain reaction allowed the isolation of a DNA probe for the DNA polymerase gene of HHV-6, which was subsequently used to isolate and position the pol gene on the physical map of the viral genome. A 4.4-kbp EcoRI DNA restriction fragment containing the pol gene was isolated and sequenced. The open reading frames flanking the pot gene code for the HHV-6 glycoprotein B gene and the human cytomegalovirus UL53 homolog. This arrangement is different from that seen in the a and y herpesvirus families, lending further support to the notion that HHV-6 is a member of the herpesvirus group.

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Human herpesvirus 6 in monocytes of transplant patients

Cited by 17 publications

References 5 publications

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Activation of human herpesvirus‐6 in children with acute measles

Characterization of the DNA polymerase gene of human herpesvirus 6

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