Human herpesvirus 6 and human herpesvirus 7 in chronic fatigue syndrome

Luca, Dario Di; Zorzenon, M.; Mirandola, Prisco; Colle, R; Botta, Giuseppe; Cassai, Enzo

doi:10.1128/jcm.33.6.1660-1661.1995

Cited by 75 publications

(28 citation statements)

References 13 publications

(9 reference statements)

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“…Data from both the civilian and veteran study populations confirmed this observation. In the literature, there are both positive (7,8,22,31) and negative (1,11,24,28) data with regard to correlation between the detection of HHV6 DNA in PBMCs of CFS patients versus healthy controls. Our present data clearly support the latter view, in that despite the use of well-characterized longterm CFS patients and an age-, race-, and gender-matched control population, there were no significant differences observed.…”

Section: Discussionmentioning

confidence: 99%

“…The primer annealing temperature was increased to 58°C and the optimum MgCl 2 concentration was determined to be 4.5 mM. The HHV7 primers used, denoted HHV7 #1 and HHV7 #2 (Table 1), amplify a 124-bp region of a conserved gene named ORF U10 (5,11). These primers are specific for HHV7 DNA alone and do not cross-react with EBV, HCMV, or either strain of HHV6 (data not shown).…”

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confidence: 99%

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Human Herpesviruses in Chronic Fatigue Syndrome

Wallace

Natelson

Gause

et al. 1999

Clin Diagn Lab Immunol

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We have conducted a double-blind study to assess the possible involvement of the human herpesviruses (HHVs) HHV6, HHV7, Epstein-Barr virus (EBV), and cytomegalovirus in chronic fatigue syndrome (CFS) patients compared to age-, race-, and gender-matched controls. The CFS patient population was composed of rigorously screened civilian and Persian Gulf War veterans meeting the Centers for Disease Control and Prevention’s CFS case definition criteria. Healthy control civilian and veteran populations had no evidence of CFS or any other exclusionary medical or psychiatric condition. Patient peripheral blood mononuclear cells were analyzed by PCR for the presence of these HHVs. Using two-tailed Fisher’s exact test analyses, we were unable to ascertain any statistically significant differences between the CFS patient and control populations in terms of the detection of one or more of these viruses. This observation was upheld when the CFS populations were further stratified with regard to the presence or absence of major axis I psychopathology and patient self-reported gradual versus acute onset of disease. In tandem, we performed serological analyses of serum anti-EBV and anti-HHV6 antibody titers and found no significant differences between the CFS and control patients.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Human Herpesviruses in Chronic Fatigue Syndrome

Wallace

Natelson

Gause

et al. 1999

Clin Diagn Lab Immunol

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“…HHV-6 may be considered ubiquitous in the human population, although some epidemiological studies in the past may have used reagents (employed to detect HHV-6 only), which may have cross-reacted with the closely related HHV-7 [Berneman et al, 1993;Ablashi et al, 1995;Di Luca et al, 1995]. Depending on geographic location, seropositivity may be greater than 80% by immunofluorescence assay (IFA) tested anti-HHV-6-IgG antibody titers of >1:20 [Levy et al, 1990;Krueger et al, 1994;Krueger et al, 1995].…”

Section: Discussionmentioning

confidence: 99%

“…The lymphotropic virus infects almost all children by the age of 2 years usually causing a lifelong latency. Independent of the patient's age HHV-6 infection has been associated with a number of neurologic conditions [Suga et al, 1993], including recurrent febrile seizures and convulsions [Kondo et al, 1993], status epilepticus [Jones et al, 1994], meningitis [Huang et al, 1991], myalgic encephalomyelitis [Wakefield et al, 1989], meningoencephalitis [Ishiguro et al, 1990;, encephalitis Kehl Knox et al, 1992], encephalopathy [Gloning et al, 1991;Asano et al, 1992], multiple sclerosis [Wilborn et al, 1994;Challoner et al, 1995], and chronic fatigue syndrome [Buchwald et al, 1992;Di Luca et al, 1995]. One work group suggests that the central nervous system may be a site of HHV-6 persistence, and that peripheral blood mononuclear cells do not play a role in hibernation of the virus [Caserta et al, 1994].…”

Section: Discussionmentioning

confidence: 99%

Human herpesvirus-6 (HHV-6)-associated necrotizing encephalitis in griscelli's syndrome

et al. 1997

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We report a male caucasian German pediatric patient of no Arab or Mediterranean ancestry with virus associated CNS lesions in Griscelli's syndrome (GS; McKusick No. 214450). The boy presented with recurrent infections, and meningitis with subsequent progressive signs of increased intracranial pressure leading to death at 32 weeks of age. At autopsy, various sites of the CNS revealed necroses in gray and white matter. CNS histology revealed numerous and massive predominantly perivascular CD8 positive lymphohistiocytic infiltrates. These findings were associated strictly with the presence of human herpesvirus-6 (HHV-6) genome or the HHV-6 specific late antigen H-AR 3, found in neurons, oligodendrocytes, and astrocytes. The search for HHV-6 replication dependent antigen, HHV-7 DNA, CMV, adenovirus, Coxsackie B1, B2, and B4-antigens, and mycobacteria was not successful. Detection of viruses was attempted using immunohistochemistry, in situ hybridization or nested polymerase chain reaction, respectively. Lymphocyte typing was carried out immunohistochemically. In GS, virus induced CNS damage does not seem to require necessarily active virus replication. It may also appear as a consequence of an immune reaction triggered by antigen expression.

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“…Med. 36:157, 1997]), fulminant hepatitis (43) Epstein-Barr virus-negative infectious mononucleosis (4,45), chronic fatigue syndrome (9,16,37), and, more recently, multiple sclerosis (2,3,11,29,44). A large body of evidence suggests that HHV-6 may act as an opportunistic agent in patients with immunodeficiencies, particularly those who have undergone bone marrow or organ transplantation (10,15,18,19,42) and human immunodeficiency virus-infected individuals (1,17,20,28,41).…”

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confidence: 99%

Real-Time Quantitative PCR for Human Herpesvirus 6 DNA

Locatelli

Santoro

Veglia³

et al. 2000

J Clin Microbiol

124

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The diagnosis of human herpesvirus 6 (HHV-6) infection represents a complex issue because the most widely used diagnostic tools, such as immunoglobulin G antibody titer determination and qualitative DNA PCR with blood cells, are unable to distinguish between latent (clinically silent) and active (often clinically relevant) infection. We have developed a new, highly sensitive, quantitative PCR assay for the accurate measurement of HHV-6 DNA in tissue-derived cell suspensions and body fluids. The test uses a 5′ nuclease, fluorogenic assay combined with real-time detection of PCR amplification products with the ABI PRISM 7700 sequence detector system. The sensitivity of this method is equal to the sensitivity of a nested PCR protocol (lower detection limit, 1 viral genome equivalent/test) for both the A and the B HHV-6 subgroups and shows a wider dynamic range of detection (from 1 to 106 viral genome equivalents/test) and a higher degree of accuracy, repeatability, and reproducibility compared to those of a standard quantitative-competitive PCR assay developed with the same reference DNA molecule. The novel technique is versatile, showing the same sensitivity and dynamic range with viral DNA extracted from different fluids (i.e., culture medium or plasma) or from tissue-derived cell suspensions. Furthermore, by virtue of its high-throughput format, this method is well suited for large epidemiological surveys.

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Human herpesvirus 6 and human herpesvirus 7 in chronic fatigue syndrome

Cited by 75 publications

References 13 publications

Human Herpesviruses in Chronic Fatigue Syndrome

Human Herpesviruses in Chronic Fatigue Syndrome

Human herpesvirus-6 (HHV-6)-associated necrotizing encephalitis in griscelli's syndrome

Real-Time Quantitative PCR for Human Herpesvirus 6 DNA

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