Human hepatocellular pigments and lysosomes

Essner, Edward; Novikoff, Alex B.

doi:10.1016/s0022-5320(60)90016-2

Cited by 318 publications

(76 citation statements)

References 16 publications

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“…2). Some of these granules still contain acid hydrolases (57)(58)(59)(60) and have a polymorphic content (61)(62)(63)(64) partly reminiscent of that of usual residual bodies.…”

Section: Lytic Cyclementioning

confidence: 99%

Lytic Activities in Renal Protein Absorption Droplets

Miller

Palade

1964

The Journal of Cell Biology

421

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The digestive cycle following reabsorption of hemoglobin by cells of the proximal convoluted tubules in mouse kidney and the uptake of ferritin by glomerular mesangial cells in the kidney of normal and nephrotic rats wereinvestigated by electron microscopical histochemical procedures.Mouse kidneys, sampled at closely spaced time points between 1 to 48 hours after intraperitoneal injection of hemoglobin, and rat (normal and nephrotic) kidneys, sampled at 30 minutes, 2 hours, and 48 hours after intravenous injection of ferritin, were fixed in glutaraldehyde, cut at 50/z on a freezing microtome, incubated for acid phosphatase and thiolacetate-esterase, and postfixed in OsO4. Satisfactory preservation of fine structure permitted the localization of the enzymatic reaction products on cell structures involved in uptake and digestion of exogenous proteins. The latter were identified either by their density (hemoglobin) or their molecular structure (ferritin).It was found that lysosomal enzymic activities and incorporated exogenous proteins occur together in the same membrane-bounded structures. In the cells of the proximal convolution, lytic activities become demonstrable within I hour after hemoglobin injection, appear first in apical vacuoles filled with hemoglobin, and persist in fully formed protein absorption droplets. At the end of the lytic cycle (~48 hours post injection), the cells have an increased population of polymorphic bodies which exhibit lytic activities. In smaller numbers, identical bodies occur in controls. It is concluded that they represent remnants of previous digestive events.The means by which the resorptive vacuoles acquire hydrolytic activities remain unknown. Fusion of newly formed vacuoles with residual bodies was not seen, and hemoglobin incorporation into such bodies was only occasionally encountered. Acid phosphatase activity was found sometimes in the Golgi complex, but enzyme transport from the complex to the resorbing vacuoles could not be established. Autolydc vacuoles containing mitochondria or mitochondrial remnants were frequently found during the early stages of hemoglobin resorption, but no definite conclusions about the mechanism involved in the segregation of endogenous material were obtained.In nephrotic rats ferritin was segregated in membrane-bounded bodies mainly in the mesangial cells and to a lesser extent in epithelial and endothelial cells. Most of these sites were marked by the reaction products of acid phosphatase and organophosphorus-resistant esterase and therefore identified as lysosomes connected with the digestion of incorporated exogenous proteins. 519on

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“…2). Some of these granules still contain acid hydrolases (57)(58)(59)(60) and have a polymorphic content (61)(62)(63)(64) partly reminiscent of that of usual residual bodies.…”

Section: Lytic Cyclementioning

confidence: 99%

Lytic Activities in Renal Protein Absorption Droplets

Miller

Palade

1964

The Journal of Cell Biology

421

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“…Until quite recently structures of this kind have been referred to in electron microscopical investigations in general terms, e.g. ' dense body ', ' micro-body', 'lamellar body' and so on, Studies on mammalian liver cell structure (Essner & Novikoff, 1960;Daems & van Rijssel, 1961) have established the identity of peribiliary dense bodies as lysosomes, i.e. membrane-limited organelles containing acid phosphatase and other enzymes, which are directly concerned in phagocytic and lytic activities of the cell (De Duve et al 1955).…”

Section: Electron Microscopymentioning

confidence: 99%

Structure and Replication of the Trachoma Agent in Cell Cultures, as shown by Electron Microscopy

Armstrong

Valentine

Fildes

1963

Journal of General Microbiology

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SUMMARYPropagation of the trachoma agent in human cell cultures has made possible the observation by electron microscopy of sequential stages in the replication of the micro-organism. Scattered reticular foci with incomplete limiting membranes were present in the cytoplasm of HeLa cells 12 hr. after exposure to the infectious agent in high multiplicity. After 24 hr. the foci had coalesced to give a single reticular mass. Within it there appeared large and discrete developmental forms from which, by progressive subdivision and diminution in size, !mica1 elementary bodies were formed. The elementary bodies had a prominent limiting membrane, and a dense core within which a fibrillar nucleoid could be recognized. The controversial taxonomic status of the trachoma agent is considered briefly in the light of ultrastructural evidence.

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“…23 In the aging L cells, larger residual bodies were occasionally observed that appeared to be formed by the fusion of several telo-lysosomes with lipid droplets. Morphologically and cytochemically, these residual bodies resembled lipofuscin granules (30, 49, 50), which have been considered by some investigators to be of lysosomal origin (51). The occasional finding of telo-lysosomes containing gold particles and membrane remnants provided evidence for an interrelationship between the phagocytotic and autodigestive pathways.…”

mentioning

confidence: 92%

Studies on the Intracellular Digestive Process in Mammalian Tissue Culture Cells

Gordon

Miller

Bensch

1965

The Journal of Cell Biology

224

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DNA-protein coacervates containing colloidal gold particles were readily phagocytizcd by strain L fibroblasts. During the subsequent digestion process, the gold particles served as markers which permitted the demonstration of the evolution of digestive vacuoles to multivesicular bodies and finally to dense bodies. Acid phosphatase and esterolytic activity was present in these structures. The hydrolytic enzymes were apparently brought to the phagocytotic vacuoles in small vesicles originating in the Golgi region. These vesicles entered the vacuoles prior to the digestion of the coacervates and the appearance of positive cytochemical reactions. The cytoplasmic dense bodies frequently merged with the phagocytotic vacuoles. This was demonstrated by prelabeling the dense bodies with colloidal iron prior to phagocytosis of the coaccrvates. In addition, evidence is presented for the interrelationship of the phagocytotic and autophagic pathways.

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Human hepatocellular pigments and lysosomes

Cited by 318 publications

References 16 publications

Lytic Activities in Renal Protein Absorption Droplets

Lytic Activities in Renal Protein Absorption Droplets

Structure and Replication of the Trachoma Agent in Cell Cultures, as shown by Electron Microscopy

Studies on the Intracellular Digestive Process in Mammalian Tissue Culture Cells

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