Human Heavy Chain Antibody Genes Elicited in Thai Dengue Patients during DENV2 Secondary Infection

Cross-reactive antibodies targeting surface-exposed non-structural protein 1 (NS1) of dengue virus-infected cells recognize epitopes on the spaghetti loop of the β-ladder domain

“…Various IGHV3 genes identified were likely due to the large IGHV3 family consisting of more than 50 genes, whereas the IGHV5 group has only a few members [ 49 , 50 ]. Moreover, the identification of the IGHV3 antibody gene was consistent with previous reports as it is the most common gene family found in DENV infection, while IGHV5-51 has been described in DENV envelope complex-epitope specific antibodies derived from memory-B cells of dengue patients [ 51 – 54 ]. Some IGHV genes form public clonotypes associated with many anti-viral antibodies, although these antibodies recognize distinct viral epitopes.…”

“…The repeated usage of this gene has been reported in DENV E and prM protein-specific antibodies, as well as polyclonal antibodies isolated from infected patients. Of interest, IGHV1-46 antibodies have not been reported to be particularly abundant in DENV infection [ 51 , 52 ], yet we identified DENV1-4 cross-reactive mAbs biased towards IGHV1-46 gene usage in this study.…”

“…The recognition of the HIV-1 envelope for these two broadly neutralizing antibodies is somewhat similar and requires interaction of the heavy chain CDR2 amino acids interface. Eliciting IGHV1-69 antibody has been widely reported in many viral infections including DENV, HIV-1, influenza A virus and H5N1 vaccination [ 51 – 54 , 57 – 59 ]. In addition, the biased usages of particular antibody genes could be exhibited in temporal dynamics and may be associated with disease severity.…”

“…In DENV infection, the over-representation of IGHV1-02 and IGHV1-69 gene usages has been reported in acute plasmablasts of patients with dengue presenting clinical warning signs [ 57 ]. IGHV1-69 antibodies are likely to be more common in dengue patients as this antibody type has been identified in convalescence stages [ 51 – 54 ]. The repeated usage of this gene has been reported in DENV E and prM protein-specific antibodies, as well as polyclonal antibodies isolated from infected patients.…”

Characterization of Human Anti-Dengue NS1 Monoclonal Antibodies Derived from Patients Infected with DENV-2

“…The monoclonal antibodies were purified from the antibody-rich supernatant with HiTrap™ Protein A HP columns and then were concentrated and buffer exchanged with Centriprep 10 kDa (Millipore, USA). Anti-NS1 HuMAb (D27-1E8A4C2; M34) was previously described, and identified as IgG1 subclass (17) This HuMAb was used as subclass control in IgG subclass identification. Dengue patient's serum derived from acute stage of DENV2 infection was used as control in endothelial cell cross-reactivity study (16).…”