Human Guanine Deaminase: Cloning, Expression and Characterisation

Snyder, Floyd F.; Yuan, Richard; Bin, James C.; Carter, Kelly L.; Mckay, Don J.

doi:10.1007/0-306-46843-3_22

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…In this pathway, seven DEGs were detected and annotated to seven proteins, including guanylyl cyclases, 3 -phosphoadenosine 5 -prime-phosphosulfate, TWISTNB protein, ectonucleoside triphosphate diphosphohydrolase, NTPDase 3, NTPDase 8, cGMP-specific-3 ,5 -cyclic phosphodiesterase, and guanine deaminase. They are mainly involved in the regulation of the nervous system (Snyder et al, 2000;Xu et al, 2000;Belcher et al, 2006). In this study, most of the DEGs in this pathway were downregulated, suggesting that the function of the nervous system is damaged, which may lead to uncoordinated movements in animals.…”

Section: Discussionmentioning

confidence: 60%

Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp

Zhang

et al. 2021

Front. Mar. Sci.

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Grass carp (Ctenopharyngodon idellus) is one of the most economically important aquaculture species and is widely cultured in China. However, its wild populations in many rivers are increasingly declining, and seawater intrusion is one of the most important threats to their survival. However, the mechanisms underlying the decline due to salinity pressure are still unknown. Here, we performed a comparative transcriptome analysis of C. idellus larvae in response to salinity exposures; a total of 481 differentially expressed genes (DEGs) were identified. These DEGs were significantly enriched in eight Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, among which steroid biosynthesis was the most important one, with the highest enrichment score. The pathway plays an important role in the development of the testes and ovary. Interestingly, all DEGs in steroid biosynthesis showed a down regulation, indicating that salinity exposure may pose damage to the fertility of C. idellus. Furthermore, three immunity-associated pathways (cytokine–cytokine receptor interaction, Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway) were also significantly enriched, suggesting impaired immunity and a high risk of disease infection under salinity exposure. Overall, damage to both fertility and immunity would decrease the number of offspring and increase the risk of death due to disease infection. Our results provide a potential molecular mechanism underlying the decline of wild C. idellus populations in the Pearl River.

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Section: Discussionmentioning

confidence: 60%

Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp

Zhang

et al. 2021

Front. Mar. Sci.

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“…Fernández et al [2009]. This sequence is common in the amidohydrolases family [Kim and Kim, 1998] and has been described in guanine deaminases of E. coli , S. cerevisiae and humans [Maynes et al, 2000;Saint-Marc and DaignanFornier, 2004;Snyder et al, 2000]. The amino acid sequence of A. adeninivorans LS3 did not, however, contain signal sequences for intracellular localisation or for secretion.…”

Section: Discussionmentioning

confidence: 99%

<b><i>Arxula adeninivorans</i></b> Recombinant Guanine Deaminase and Its Application in the Production of Food with Low Purine Content

Trautwein-Schult

Jankowska²,

Cordes³

et al. 2014

Microb Physiol

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Purines of exogenous and endogenous sources are degraded to uric acid in human beings. Concentrations >6.8 mg uric acid/dl serum cause hyperuricemia and its symptoms. Pharmaceuticals and the reduction of the intake of purine-rich food are used to control uric acid levels. A novel approach to the latter proposition is the enzymatic reduction of the purine content of food by purine-degrading enzymes. Here we describe the production of recombinant guanine deaminase by the yeast Arxula adeninivorans LS3 and its application in food. In media supplemented with nitrogen sources hypoxanthine or adenine, guanine deaminase (AGDA) gene expression is induced and intracellular accumulation of guanine deaminase (Agdap) protein occurs. The characteristics of the guanine deaminase isolated from wild-type strain LS3 and a transgenic strain expressing the AGDA gene under control of the strong constitutive TEF1 promoter were determined and compared. Both enzymes were dimeric and had temperature optima of 55°C with high substrate specificity for guanine and localisation in both the cytoplasm and vacuole of yeast. The enzyme was demonstrated to reduce levels of guanine in food. A mixture of guanine deaminase and other purine degradation enzymes will allow the reduction of purines in purine-rich foods.

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“…This often leads to the formation of specific chemical motifs that are unique and determinant for the protein function . The enzyme guanine deaminase is one of these cases with a zinc ion surrounded by a cluster of histidine residues in its active site . The X‐ray structure with the bound product (pdb code: 2UZ9) also reveals that the metal ion interacts very closely with the substrate, and therefore, it participates directly in the catalytic process.…”

Section: Software Validationmentioning

confidence: 99%

chem‐path‐tracker: An Automated Tool to Analyze Chemical Motifs in Molecular Structures

et al. 2014

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In this article, we propose a method for locating functionally relevant chemical motifs in protein structures. The chemical motifs can be a small group of residues or structure protein fragments with highly conserved properties that have important biological functions. However, the detection of chemical motifs is rather difficult because they often consist of a set of amino acid residues separated by long, variable regions, and they only come together to form a functional group when the protein is folded into its three-dimensional structure. Furthermore, the assemblage of these residues is often dependent on non-covalent interactions among the constituent amino acids that are difficult to detect or visualize. To simplify the analysis of these chemical motifs and give access to a generalized use for all users, we developed chem-path-tracker. This software is a VMD plug-in that allows the user to highlight and reveal potential chemical motifs requiring only a few selections. The analysis is based on atoms/residues pair distances applying a modified version of Dijkstra's algorithm, and it makes possible to monitor the distances of a large pathway, even during a molecular dynamics simulation. This tool turned out to be very useful, fast, and user-friendly in the performed tests. The chem-path-tracker package is distributed as an independent platform and can be found at http://www.fc.up.pt/PortoBioComp/database/doku.php?id=chem-path-tracker.

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Human Guanine Deaminase: Cloning, Expression and Characterisation

Cited by 7 publications

References 17 publications

Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp

Transcriptome Analysis Reveals Impaired Fertility and Immunity Under Salinity Exposure in Juvenile Grass Carp

<b><i>Arxula adeninivorans</i></b> Recombinant Guanine Deaminase and Its Application in the Production of Food with Low Purine Content

chem‐path‐tracker: An Automated Tool to Analyze Chemical Motifs in Molecular Structures

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