Human genomic DNA isolation from whole blood using a simple microfluidic system with silica- and polymer-based stationary phases

Günal, Gülçin; Kip, Çiğdem; Öğüt, Sevim Eda; Usta, Duygu Deniz; Şenlik, Erhan; Kibar, Güneş; Tuncel, Alï

doi:10.1016/j.msec.2016.12.118

Cited by 31 publications

(23 citation statements)

References 32 publications

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“…By performing an elemental analysis, carbon and nitrogen contents of bare SiO 2 microspheres were determined as 0.11 and 0.46% w/w, respectively. Carbon and nitrogen contents of bare SiO 2 microspheres should come from the poly(MAA‐co‐EDMA) seed particles and TBAI used as the starting material and the stabilizer, respectively, in the synthesis . On the other hand, carbon and the nitrogen contents of MIPS@SiO 2 microspheres were found as 2.92 and 0.54% w/w, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Monodisperse‐porous silica microspheres with a mean size of 5.4 μm were obtained by a staged shape templated hydrolysis and condensation method . An imprinting technique based on both free radical polymerization and boronate affinity interaction was used for the synthesis of a sorbent for selective adsorption of β‐NAD.…”

Section: Resultsmentioning

confidence: 99%

“…Monodisperse‐porous silica microspheres (bare SiO 2 microspheres) were synthesized by a staged shape templated hydrolysis‐condensation method by using monodisperse‐porous poly(MAA‐co‐EDMA) microspheres as the seed particles .…”

Section: Methodsmentioning

confidence: 99%

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Molecularly imprinted polymeric shell coated monodisperse‐porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography

Süngü

Kip

Tuncel

2019

J of Separation Science

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Molecular imprinting of cis-diol functionalized agents via boronate affinity interaction has been usually performed using nanoparticles as a support which cannot be utilized as a stationary phase in continuous microcolumn applications. In this study, monodisperse-porous, spherical silica particles in the micron-size range, with bimodal pore diameter distribution were selected as a new support for the synthesis of a molecularly imprinted boronate affinity sorbent, using a cis-diol functionalized agent as the template. A specific surface area of 158 m 2 /g was achieved with the imprinted sorbent by using monodisperse-porous silica microspheres containing both mesoporous and macroporous compartments as the support. High porosity originating from the macroporous compartment and sufficiently high particle size provided good column permeability to the imprinted sorbent in microcolumn applications. The mesoporous compartment provided a large surface area for the parking of imprinted molecules while the macroporous compartment facilitated the intraparticular diffusion of imprinted target within the microsphere interior. A microfluidic boronate affinity system was first constructed by using molecularly imprinted polymeric shell coated monodisperse-porous silica microspheres as a stationary phase. The synthetic route for the imprinting process, the reversible adsorption/ desorption behavior of selected target and the selectivity of imprinted sorbent in both batch and microfluidic boronate affinity chromatography systems are reported. K E Y W O R D Sadsorption, microfluidic systems, molecular imprinting, nucleotides, solid-phase extraction Article Related Abbreviations: EDMA, ethylene dimethacrylate; EDX, Energy Dispersive X-Ray Spectroscopy; HEPES, hydroxyethylpiperazine ethanesulfonic acid; IF, Imprinting factor; MAA, methacrylic acid; MIPS@SiO 2 microspheres, molecularly imprinted polymeric shell coated silica microspheres, by using β-NAD as the template; NIPS@SiO 2 microspheres, non-imprinted polymeric shell coated silica microspheres; β-NAD, β-nicotinamide adenine dinucleotide; SSA, specific surface area; TMSPM, 3-trimethoxysilylpropyl methacrylate; VPBA, 4-vinylphenylboronic acid.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Molecularly imprinted polymeric shell coated monodisperse‐porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography

Süngü

Kip

Tuncel

2019

J of Separation Science

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“…hydrolysis and condensation protocol [64,65]. These supports had a bimodal pore-size distribution, appropriate porosity, and high surface area (> 250 m 2 /g) [64,65].…”

Section: F I G U R E 2 Bioaffinity Chromatography Methods Target Biomentioning

confidence: 99%

“…Monodisperse-porous metal oxide microspheres, synthesized by staged-shape templated hydrolysis and condensation protocol or staged sol-gel templating protocol, and capable of interacting with the biomolecules via MOAC were also evaluated for their isolation or colorimetric detection [64,[89][90][91]. Monodisperse-porous silica microspheres with a mean size of ca 5.0 μm and bimodal pore size distribution were used as stationary phase in a microfludic system for isolation of human genomic DNA (hgDNA) from human whole blood via MOAC [64]. The comparison of DNA isolation characteristics of synthesized silica microspheres with the polymer based sorbents in the form of uniform-porous microspheres with similar size and porous characteristics was made in batch fashion [89].…”

Section: F I G U R Ementioning

confidence: 99%

Recent trends in sorbents for bioaffinity chromatography

Kip

Hamaloğlu

Demir

et al. 2021

J of Separation Science

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Isolation or enrichment of biological molecules from complex biological samples is mostly a prerequisite in proteomics, genomics, and glycomics. Different techniques have been used to advance the efficiency of the purification of biological molecules. Bioaffinity chromatography is one of the most powerful technique that plays an important role in the isolation of target biological molecules by the specific interactions with ligands that are immobilized on different support materials. This review examines the recent developments in bioaffinity chromatography particularly over the past 5 years in the literature. Also properties of supports, immobilization techniques, types of binding agents, and methods used in bioaffinity chromatography applications are summarized.

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Microfluidic systems for the analysis of blood‐derived molecular biomarkers

Chavez-Pineda¹,

Rodriguez‐Moncayo²,

Cedillo-Alcantar³

et al. 2022

Electrophoresis

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Biomarkers are relevant indicators of the physiological state of an individual.Although biomarkers can be found in diseased tissue and different biofluids, sampling from blood plasma is relatively easy and less invasive. Among the molecular biomarkers that can be found circulating in plasma are proteins, metabolites, nucleic acids, and exosomes. Some of these plasma-circulating biomarkers are now employed for patient stratification in a broad range of diseases with high sensitivity and specificity and are useful in early diagnosis, initial risk assessment, and therapy selection. However, there is a pressing need to develop novel approaches for biomarker analysis that can be translated into clinical or other settings without complex methodologies or instrumentation. Microfluidics has been touted as a promising technology to carry out this task because it offers high-throughput, automation, multiplexed detection, and portability, possibly overcoming the bottleneck that prevent the translation of novel biomarkers to the point-of-care (POC). Here, we provide a review of the microfluidic systems that have been engineered to detect circulating molecular biomarkers in blood plasma. We also review the different microfluidic approaches for plasma enrichment, which are now being integrated with microfluidic-based biomarker analyzers. Such integration should lead to

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Human genomic DNA isolation from whole blood using a simple microfluidic system with silica- and polymer-based stationary phases

Cited by 31 publications

References 32 publications

Molecularly imprinted polymeric shell coated monodisperse‐porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography

Molecularly imprinted polymeric shell coated monodisperse‐porous silica microspheres as a stationary phase for microfluidic boronate affinity chromatography

Recent trends in sorbents for bioaffinity chromatography

Microfluidic systems for the analysis of blood‐derived molecular biomarkers

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