Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene

Cao, Lequn; Taggart, R T; Berquin, Isabelle M.; Moin, Kamiar; Fong, Dunne; Sloane, Bonnie F.

doi:10.1016/0378-1119(94)90750-1

Cited by 51 publications

(33 citation statements)

References 23 publications

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“…In accordance with the results of the present study, TIMP1 was also identified to be associated with interstitial pneumonia (43,44). CTSB belongs to a family of lysosomal cysteine proteases and is encoded by the CTSB gene in humans (45,46). It is an important endogenous protease in intracellular proteolysis, regulating cell apoptosis and restricting injury-associated inflammation (47).…”

Section: Discussionsupporting

confidence: 89%

Gene expression analysis for pneumonia caused by Gram-positive bacterial infection

et al. 2018

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Abstract. Gram-positive bacteria are an important pathogenic factor for bacterial pneumonia. The aim of the present study was to identify the differentially expressed genes (DEGs) and to explore their associated pathways or expression patterns. Expression profiling of gene arrays from two independent datasets, GSE6269 and GSE35716, were downloaded from the Gene Expression Omnibus. The DEGs between peripheral blood samples from healthy controls and patients with bacterial pneumonia were identified. The Functional Annotation Tool in the Database for Annotation, Visualization and Integrated Discovery was used to annotate and analyze the DEGs in Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Multiple proteins were used to generate a protein-protein interaction (PPI) network. A total of 624 (621 annotated) were identified in the GSE6269 dataset and 398 (295 annotated) DEGs were identified in the GSE35716 dataset between pneumonia and healthy samples. A total of 40 common DEGs were identified between the 2 datasets, including 4 downregulated and 32 upregulated DEGs. In the GO category cellular component, melanosome was highly enriched among 11 genes; in the category biological process, the three most enriched items were regulation of ruffle assembly, negative regulation of calcium ion transport and necroptotic process. In the KEGG terms, only the nuclear factor-κB signaling pathway (Homo sapiens 04064) was significantly enriched. In the PPI network, five genes (CCL4, TIMP metallopeptidase inhibitor 1, intercellular adhesion molecule 1, plasminogen activator, urokinase receptor and cathepsin B) were identified to have a high degree of interaction with other DEGs. In conclusion, these five genes may represent key genes associated with pneumonia caused by Gram-positive bacteria. All of these results provide primary information and basic knowledge to understand the mechanisms of the pathogenesis.

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Section: Discussionsupporting

confidence: 89%

Gene expression analysis for pneumonia caused by Gram-positive bacterial infection

et al. 2018

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“…A 1.15-kb cDNA fragment coding for cathepsin B (Cao et al, 1994) was blunt end cloned in the antisense orientation into the HindIII site of the eukaryotic expression vector pH-b-Aprneo-1 (Gunning et al, 1987). Stable antisense transfectants were generated and characterized as described earlier (Mohanam et al, 2001).…”

Section: Preparation Of Constructs and Transfectionmentioning

confidence: 99%

Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis

et al. 2004

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The cysteine proteinase cathepsin B has been implicated in tumor progression by virtue of its increased mRNA and protein levels, as well as its localization at the invading front of the tumor. In this study, we examined whether blocking cathepsin B expression in human glioblastoma SNB19 cells affects angiogenesis. Stable transfectants of human glioblastoma cells with a plasmid containing antisense cathepsin B cDNA showed decreased migration rates in wound-and spheroid-migration assays. Analysis showed a reduction in VEGF protein and MMP-9 activity in the cathepsin B antisense cDNA-transfected cells. Regarding angiogenesis in vitro, we found that the conditioned medium of glioblastoma cells with downregulated cathepsin B expression reduced cell-cell interaction of human microvascular endothelial cells, resulting in the disruption of capillary-like network formation. Furthermore, a marked reduction in microvasculature development was seen in an in vivo dorsal air sac assay of glioblastoma cells with downregulated cathepsin B expression. Taken together, these results provide evidence that inhibition of cathepsin B expression can suppress glioblastoma-induced neovascularization.

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“…Probes for human proteinase and uPAR genes included: MMP-9 (92 kDa Type IV collagenase) (1046 bp insert from plasmid p92MO1) (gift of Dr WG StetlerStevenson), MMP-2 (72 kDa Type IV collagenase) (1117 bp insert from plasmid p3Ha) (Reponen et al, 1992), cathepsin B (1.6 kb KpnI insert from plasmid pLC343) (gift of Dr B Sloane) (Cao et al, 1994), cathepsin D (20 kb insert from plasmid pM13mp10) (gift of Dr H Rochefort) (Augereau et al, 1988), cathepsin L (800 bp insert from plasmid pHCL800.1) (gift of Dr DT Denhardt) (Joseph et al, 1988), urokinase-type plasminogen activator (40mer antisense oligonucleotide derived from the translated sequences of exon 4) (Calbiochem/Cederlane Laboratories, Hornby, Ontario, Canada, Cat#ON333) (Riccio et al, 1985), urokinase-type plasminogen activator receptor (45mer antisense oligonucleotide probe to the ®rst 15 amino acids (not including the signal peptide) (Roldan et al, 1990). Even loading of lanes was con®rmed by probing blots with a human 18S rRNA probe (p100D9; a kind gift from Dr DT Denhardt).…”

Section: Rna Isolation and Northern Blot Analysismentioning

confidence: 99%

Osteopontin induces increased invasiveness and plasminogen activator expression of human mammary epithelial cells

et al. 1999

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Osteopontin (OPN) has been associated with enhanced malignancy in breast cancer, but its functional role in this disease is poorly understood. To study the e ect of OPN on cellular invasiveness, basal OPN expression was ®rst assessed in members of a progression series of human mammary epithelial cell lines (21PT: immortalized, non-tumorigenic; 21NT: weakly tumorigenic; 21MT-1: tumorigenic, weakly metastatic; MDA-MB-435 cells: tumorigenic, highly metastatic). The two lines which expressed lowest basal levels of OPN (21PT, 21NT) were then examined for up-regulation of invasive behavior in response to exogenous or transfected (endogenous) OPN. Both 21PT and 21NT showed increased invasiveness through Matrigel when human recombinant (hr)OPN was added to the lower chamber of transwells. Both also showed a cell migration response to hrOPN. Populations of 21PT and 21NT cells stably transfected with an OPN-expression vector showed higher levels of cell invasiness than control vector transfectants. Examination of transfectants for mRNA of a number of secreted proteases showed that only urokinase-type plasminogen activator (uPA) expression was closely associated with OPN expression and cellular invasiveness. Treatment of the parental 21PT and 21NT cells with exogenous hrOPN resulted in increased uPA mRNA expression and increased urokinase activity of the conditioned media. Both increased cell migration and induction of uPA expression are thus potential mechanisms of increased invasiness of breast epithelial cells in response to OPN.

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Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene

Cited by 51 publications

References 23 publications

Gene expression analysis for pneumonia caused by Gram-positive bacterial infection

Gene expression analysis for pneumonia caused by Gram-positive bacterial infection

Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis

Osteopontin induces increased invasiveness and plasminogen activator expression of human mammary epithelial cells

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