Human Foreskin Fibroblast Behavior in the Presence of 3D Collagen, Fibrin, and Calcium Phosphate

Abstract

3D modeling in cell‐based assays, provides an environment that mimics in vivo conditions. This allows for the examination of innate cellular behavior and aids in the development of next‐generation modeling and therapies. Cellular environments can be modeled using various constituents to manipulate the rigidity, porosity, and size of various 3D environments to observe cell characteristics in an environment similar to that from which they originated. The proliferation of Human Foreskin Fibroblasts (HFF‐1) were examined in a 3D collagen matrix with varying cell densities. Higher cell concentration within the 3D collagen matrix showed lower HFF‐1 cell proliferation. Furthermore, fibrin clots were used to mimic 3D microenvironments because of its biocompatibility and controllable biodegradability. Changing fibrinogen and thrombin concentrations provides a varying effect on the rigidity and porosity of a 3D environment and therefore affects the overall behavior of HFF‐1. There was an increase in fibrin bead size in the presence of cells with higher concentrations of fibrinogen and thrombin. To mimic a bone microenvironment, calcium phosphate (CaP) was introduced in increasing concentrations to support a rigid and structured environment. Increasing concentrations of CaP, resulted in an increase in the initial adhesion and a decrease in HFF‐1 proliferation over a 7‐day incubation period. Overall, cells in a 2D environment behave different than those in a 3D environment with varying components to imitate their in vivo conditions.Support or Funding InformationN/AThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Fibrin sealant as a delivery vehicle for cells, antibiotics, growth factors, and painkillers

“…This composition of fibrin can then be optimized for controlled release, and various formulations of fibrin sealant can be chosen for co-delivery. 33,[99][100][101] This wound healing model may be a future application for how fibrin sealant can be used to both heal a wound site and act as a delivery system for multiple loaded components. DiD on the surface of the 3D wound healing construct and near the fibrinfilled defect at a concentration of 300,000 cells/mL; B fibroblasts fluorescentlytagged with Vybrant DiO that are encapsulated inside a fibrin bead within the collagen layers of the 3D wound healing construct and near the fibrinfilled defect (20,000 cells/bead); and C a stacked fluorescent image of the keratinocyte-fibroblast bead co-culture with fibroblasts encapsulated inside fibrin beads.…”