Human fibrin tissue adhesive for sutureless lamellar keratoplasty and scleral patch adhesion

Kaufman, Herbert E.; Insler, Michael S.; Ibrahim-Elzembely, Hosan A; Kaufman, Stephen C.

doi:10.1016/s0161-6420(03)00832-7

Cited by 110 publications

(60 citation statements)

References 8 publications

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“…Fibrin promotes cell attachment and spreading on itself in vitro; the fibrin deposition immediately after corneal injury precedes the deposition of more permanent matrix proteins involved in corneal wound repair like collagen IV and laminin (30) . Fibrin was successfully used in humans for sutureless lamellar keratoplasty associated with contact lens bandage resulting in clear grafts, confirming the experimental results in rabbits (31)(32) . The time elapsed to repair the corneal epithelium in PRK can influence the degree of corneal haze (33) .…”

Section: Perform a Literature Search To Theoretically Justify Studiessupporting

confidence: 67%

A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK)-operated corneas

Bonatti¹,

Bechara²,

Dall'Col³

et al. 2007

Arq. Bras. Oftalmol.

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Section: Perform a Literature Search To Theoretically Justify Studiessupporting

confidence: 67%

A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK)-operated corneas

Bonatti¹,

Bechara²,

Dall'Col³

et al. 2007

Arq. Bras. Oftalmol.

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“…To avoid suture-related disadvantages and complications, fibrin-based tissue adhesives are increasingly used as a sutureless alternative in various ocular surgeries including conjunctival autograft for pterygium, 13 lamellar keratoplasty, 14,15 cataract wound closure, 16 stem cell transplantation, 17 and laser in situ keratomilieusis (LASIK) surgery. [18][19][20] Fibrin glue has also been successfully used to secure AM in cases with corneal ulcers or perforations, 21,22 scleral melt, 23 conjunctivochalasis, 24,25 and pterygium.…”

mentioning

confidence: 99%

Sutureless Amniotic Membrane Transplantation for Partial Limbal Stem Cell Deficiency

Kheirkhah

Casas

Raju

et al. 2008

American Journal of Ophthalmology

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PURPOSE-To evaluate the results of sutureless amniotic membrane (AM) transplantation using fibrin glue for reconstructing corneal surfaces with partial limbal stem cell deficiency (LSCD). DESIGN-Retrospective noncomparative interventional case series.METHODS-Eleven eyes of nine patients that had LSCD with 120 degrees to almost 360 degrees of limbal involvement underwent superficial keratectomy to remove the conjunctivalized pannus followed by AM transplantation using fibrin glue. Additional sutureless AM patch (ProKera; BioTissue, Inc, Miami, Florida, USA) was used in seven patients, and mitomycin C was applied on the cornea in four eyes and during fornix reconstruction in seven eyes. The surgery was repeated in three eyes for residual pannus.RESULTS-During a mean follow-up of 14.2 ± 7.7 months (range, six to 26 months), all eyes maintained a smooth and stable corneal epithelial surface without recurrent erosion or persistent epithelial defect, and showed less stromal cloudiness and vascularization. Best-corrected visual acuity improved in nine eyes (81.8%). Corneal epithelialization proceeded by epithelial growth over AM (n = 4), accompanied by dissolution of AM (n = 4) or a combination of both (n = 3). No complication was noted regarding initial or repeated uses of fibrin glue.CONCLUSION-AM transplantation using fibrin glue appears to be a safe and effective method of restoring a stable corneal epithelium for cases with partial LSCD. This approach avoids the need of transplanting limbal epithelial stem cells. THE MAINTENANCE OF A HEALTHY CORNEAL EPITHElium under both normal and traumatic conditions is provided by a unique subpopulation of stem cells located at the limbus. 1,2 When limbal epithelial stem cells are destroyed and/or their supporting stromal environment becomes dysfunctional, a pathological state known as limbal stem cell deficiency (LSCD) develops (for review; see Reference 3 ). LSCD carries the cytologic evidence of conjunctivalization of the corneal surface by a goblet cell-containing conjunctival epithelium. 4 Pathologically, limbal-deficient corneas exhibit destruction of the basement membrane, superficial neovascularization, chronic inflammation, scarring, and poor epithelial integrity (for reviews; see References 3 and 5 ). Clinically, LSCD is a major cause of corneal blindness unable to be corrected by traditional corneal transplantation alone.For eyes inflicted with partial LSCD, where a part of the limbus is damaged, the corneal surface can be reconstructed by debridement of conjunctivalized epithelium with 6-8 or without 9 transplantation of amniotic membrane (AM). Several studies have shown that in eyes with partial LSCD, AM promotes expansion of remaining limbal epithelial stem cells. [6][7][8]10 NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript these studies, sutures were used to secure AM to the cornea following the removal of conjunctivalized pannus from the corneal surface. Recent findings suggest that epithelialization may occur both over and unde...

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“…Lem fibronektin komersial sebagai pengganti jahitan banyak memberikan keuntungan, antara lain waktu operasi lebih singkat, serta mempercepat penempelan jaringan dan penyembuhan luka. 3 Sampai saat ini LFK belum tersedia di Indonesia karena harus diimpor dengan harga yang mahal dan perlu penyimpanan khusus. Food and Drug Administration (FDA) belum secara resmi mengeluarkan izin untuk pemakaian LFK pada operasi mata karena terbuat dari protein asing yang dikhawatirkan akan menularkan penyakit.…”

Section: Pendahuluanunclassified

Peran Lem Fibrin Otologus pada Penempelan Tandur Konjungtiva Bulbi Mata Kelinci terhadap Ekspresi Gen Fibronektin dan Integrin

Enus¹,

Natadisastra²,

Shahib³

et al. 2011

mkb

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AbstrakPenempelan jaringan dan penyembuhan luka pada cangkok konjungtiva lebih cepat pada teknik lem fibrin otologus (LFO) dibandingkan dengan teknik jahitan. Kedua proses tersebut memerlukan interaksi fibronektin (FN) dan integrin α 5 yang mengaktivasi alur persinyalan intraselular. Tujuan penelitian untuk menentukan kekuatan ekspresi gen FN serta integrin α 5 pada kelompok teknik LFO dan jahitan. Uji eksperimental hewan pada kelinci New Zealand White yang terbagi kelompok teknik LFO dan jahitan masing-masing 8 kelinci bertempat di Laboratorium Sentral (Biologi Molekuler) FK Unpad Bandung, periode Mei-Oktober 2008. Sampel jaringan untuk pemeriksaan reverse transcriptase polymerase chain reaction (RT-PCR) diambil dari eksterpasi satu hari sesudah jaringan cangkok konjungtiva bulbi. Analisis data untuk uji hipotesis dengan Mann Whitney for small sample. Ekspresi gen messenger ribonucleic acid (mRNA) FN secara bermakna lebih kuat pada teknik LFO dibandingkan dengan teknik jahitan (1,9 vs 1,0; p=0,014). Tidak terdapat perbedaan bermakna ekspresi gen (mRNA) integrin α 5 antara teknik LFO dan teknik jahitan (1,2 vs 1,0; p=0,235). Sebagai simpulan ekspresi gen FN lebih kuat pada teknik LFO dibandingkan dengan jahitan, sedangkan ekspresi gen integrin α 5 pada teknik LFO lebih kuat dibandingkan dengan teknik jahitan namun secara statistik tidak bermakna satu hari pascabedah. [MKB. 2011;43(4)

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Human fibrin tissue adhesive for sutureless lamellar keratoplasty and scleral patch adhesion

Cited by 110 publications

References 8 publications

A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK)-operated corneas

A fibrin-related line of research and theoretical possibilities for the use of fibrin glue as a temporary basal membrane in non-perforated corneal ulcers and in photorefractive keratectomy (PRK)-operated corneas

Sutureless Amniotic Membrane Transplantation for Partial Limbal Stem Cell Deficiency

Peran Lem Fibrin Otologus pada Penempelan Tandur Konjungtiva Bulbi Mata Kelinci terhadap Ekspresi Gen Fibronektin dan Integrin

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