Human Fascioliasis in Shropshire

Ashton, W. L. G.; Boardman, Philip; D'Sa, C J; Everall, P. H.; Houghton, A. W. J.

doi:10.1136/bmj.3.5721.500

Cited by 36 publications

(13 citation statements)

References 3 publications

(2 reference statements)

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“…Worldwide, Fasciola hepatica, a liver fluke, is a causative agent of fascioliasis in mammals (11,34,49), including humans (2,15,38). Following ingestion of metacercaria by the hosts, the juvenile worms burrow through the host gut walls and migrate to the liver, where they cause extensive damage before moving into the bile ducts.…”

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Molecular Cloning and Expression of Cu/Zn-Containing Superoxide Dismutase from Fasciola hepatica

Kim

Jung

et al. 2000

Infect Immun

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The cytosolic superoxide dismutase (SOD) of Fasciola hepatica, a causative agent of fascioliasis, was purified and characterized. The enzyme consists of two identical subunits, each with an apparent molecular mass of 17.5 kDa. An analysis of the enzyme's primary structure and inhibition studies revealed that the enzyme is a copper/zinc-containing SOD (Cu/Zn-SOD). The enzyme activity was relatively stable in a broad pH range, from pH 7.0 to 10.0, and the enzyme showed maximum activity at pH 7.5. This enzyme also displayed strong antigenicity against sera of bovine and human subjects with fascioliasis. The SOD gene fragment was amplified by PCR with degenerate oligonucleotide primers derived from amino acid sequences conserved in the Cu/ZnSODs of other organisms. An F. hepatica cDNA library was screened with the SOD gene fragment as a probe. As a result, a complete gene encoding the Cu/Zn-SOD was identified, and its nucleotide sequence was determined. The gene had an open reading frame of 438 bp and 146 deduced amino acids. Comparison of the deduced amino acid sequence of the enzyme with previously reported Cu/Zn-SOD amino acid sequences revealed considerably high homologies. The coding region of the F. hepatica Cu/Zn-SOD was cloned and expressed in Escherichia coli. Staining of native polyacrylamide gel for SOD activity of the expressed protein revealed SOD activity that was inactivated by potassium cyanide and hydrogen peroxide but not by sodium azide. This means that the presence of the recombinant fusion protein is indicative of Cu/Zn-SOD. The expressed protein also reacted with sera of bovine and human subjects with fascioliasis, but it did not react with sera of uninfected bovine and human subjects.

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Molecular Cloning and Expression of Cu/Zn-Containing Superoxide Dismutase from Fasciola hepatica

Kim

Jung

et al. 2000

Infect Immun

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“…Watercress is a well-recognised vehicle of transmission for fascioliasis in many countries [9][10][11][12][13] and a study of microbial contamination of pre-harvest watercress in New Zealand found high levels of E. coli and Campylobacter in watercress and growing water [14]. A case-control study of over 350 cases of STEC infection reported in England from 1996 to 1997 identified watercress as a risk factor for STEC infection [15]; however, as far as we are aware, this is the first known outbreak of STEC infection associated with watercress.…”

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Outbreak of Shiga toxin-producing E. coli O157 associated with consumption of watercress, United Kingdom, August to September 2013

et al. 2013

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“…The recent outbreak of human fascioliasis in Shropshire (Ashton, Boardman, D'Sa, Everall, and Houghton, 1970) provided the stimulus and some of the material for an investigation of the value of the double diffusion precipitin test in this disease.…”

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