Human extravillous trophoblast invasion: intrinsic and extrinsic regulation

Menkhorst, Ellen; Winship, Amy; Sinderen, Michelle Van; Dimitriadis, Evdokia

doi:10.1071/rd14208

Cited by 36 publications

(30 citation statements)

References 102 publications

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“…The local decidual environment plays a key role in regulating trophoblast invasion 2 , 4 . Prior to implantation and in preparation for pregnancy, stromal cells of the uterine endometrium become ‘decidualized’.…”

Section: Introductionmentioning

confidence: 99%

Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Menkhorst

Sinderen

Rainczuk

et al. 2017

Sci Rep

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During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.

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Section: Introductionmentioning

confidence: 99%

Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Menkhorst

Sinderen

Rainczuk

et al. 2017

Sci Rep

Self Cite

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“…Trophoblast cells are fundamentally involved in embryo implantation and placental development. At an early stage of pregnancy, extravillous trophoblast cells come up from the villus and penetrate into the maternal decidua and induce remodeling of the uterine spiral arteries [ 1 – 3 ]. Disorders during pregnancy such as preeclampsia and intrauterine growth restriction (IUGR) involve the dysfunction of trophoblast cells.…”

Section: Introductionmentioning

confidence: 99%

Effects of Human Umbilical Cord Mesenchymal Stem Cells on Human Trophoblast Cell Functions In Vitro

Huang

Chang

et al. 2016

Stem Cells International

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Trophoblast cell dysfunction is involved in many disorders during pregnancy such as preeclampsia and intrauterine growth restriction. Few treatments exist, however, that target improving trophoblast cell function. Human umbilical cord mesenchymal stem cells (hUCMSCs) are capable of self-renewing, can undergo multilineage differentiation, and have homing abilities; in addition, they have immunomodulatory effects and paracrine properties and thus are a prospective source for cell therapy. To identify whether hUCMSCs can regulate trophoblast cell functions, we treated trophoblast cells with hUCMSC supernatant or cocultured them with hUCMSCs. Both treatments remarkably enhanced the migration and invasion abilities of trophoblast cells and upregulated their proliferation ability. At a certain concentration, hUCMSCs also modulated hCG, PIGF, and sEndoglin levels in the trophoblast culture medium. Thus, hUCMSCs have a positive effect on trophoblast cellular functions, which may provide a new avenue for treatment of placenta-related diseases during pregnancy.

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“…9 In order to better understand and quantify the complex factors regulating trophoblast invasion and migration, a wide range of in vitro models of trophoblast migration have been employed.…”

Section: Studying Trophoblast Migration In Vitromentioning

confidence: 99%

Quantifying trophoblast migration: In vitro approaches to address in vivo situations

James

Tun

Clark

2016

Cell Adhesion & Migration

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When trophoblasts migrate and invade in vivo, they do so by interacting with a range of other cell types, extracellular matrix proteins, chemotactic factors and physical forces such as fluid shear stress. These factors combine to influence overall trophoblast migration and invasion into the decidua, which in turn determines the success of spiral artery remodeling, and pregnancy itself. Our understanding of these important but complex processes is limited by the simplified conditions in which we often study cell migration in vitro, and many discrepancies are observed between different in vitro models in the literature. On top of these experimental considerations, the migration of individual trophoblasts can vary widely. While time-lapse microscopy provides a wealth of information on trophoblast migration, manual tracking of individual cell migration is a time consuming task that ultimately restricts the numbers of cells quantified, and thus the ability to extract meaningful information from the data. However, the development of automated imaging algorithms is likely to aid our ability to accurately interpret trophoblast migration in vitro, and better allow us to relate these observations to in vivo scenarios. This commentary discusses the advantages and disadvantages of techniques commonly used to quantify trophoblast migration and invasion, both from a cell biology and a mathematical perspective, and examines how such techniques could be improved to help us relate trophoblast migration more accurately to in vivo function in the future.

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Human extravillous trophoblast invasion: intrinsic and extrinsic regulation

Cited by 36 publications

References 102 publications

Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

Effects of Human Umbilical Cord Mesenchymal Stem Cells on Human Trophoblast Cell Functions In Vitro

Quantifying trophoblast migration: In vitro approaches to address in vivo situations

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