Human exposure to low dose ionizing radiation affects miR-21 and miR-625 expression levels

Mahmoudi, Roghayeh; Saidijam, Massoud; Nikzad, Safoora; Tapak, Leili; Alvandi, Maryam; Afshar, Saeid

doi:10.1007/s11033-021-06960-3

Cited by 7 publications

(3 citation statements)

References 46 publications

(43 reference statements)

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“…The present study showed that the predicted target gene of miR-21 is SP1, which also makes sense in theory. To evaluate the role of miR-21 in response to low-dose ionizing radiation, a study was carried out on 38 volunteer patients, and bioinformatics analysis indicated that miR-21 can contribute to the response to acute low-dose ionizing radiation by targeting the SP1 (Mahmoudi et al, 2022 ). Yang et al found that miR-21 regulates CSE protein expression in vascular smooth muscles by regulating SP1 genes (Yang et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Whole-Blood MicroRNA Sequence Profiling and Identification of Specific miR-21 for Adolescents With Postural Tachycardia Syndrome

Lin

Shen²,

Liu³

et al. 2022

Front. Neurosci.

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ObjectiveThe aim of the study was to establish whether whole-blood microRNA (miRNA) profiles differ between postural tachycardia syndrome (POTS) sufferers and control subjects and to identify the miRNA that regulates plasma H2S.Study DesignHigh-throughput sequencing was used to obtain whole-blood miRNA expression profiles for 20 POTS sufferers and 20 normal children.The thresholds for defining differentially expressed miRNAs (DEmiRNAs) were an adjusted DESeq P of <0.05 and a log2 fold variation of ≥3. The DEmiRNA target genes were identified using RNAhybrid and miRanda, and only those identified by both were considered. The combined effects of the DEmiRNAs were determined using KEGG pathway analysis. Another 40 POTS and 20 normal patients were used as validation subjects. Plasma H2S was determined with a sulfide electrode, and flow-mediated vasodilation (FMD) was performed with a color Doppler ultrasound system. miRNAs were analyzed using qRT-PCR.ResultsTotally, 13 DEmiRNAs were identified through high-throughput sequencing. In the 60-member validation group, the 13 miRNAs were verified again, and it turned out that miR-21 was significantly elevated and could diagnose POTS with a 100% specificity and 92.5% sensitivity. Overall, 198 and 481 genes, respectively, were shown to be targeted by the 13 DEmiRNAs when P values of 0.01 and 0.05 were used. The target gene of hsa-miR-21-5p was SP1 when the P-value is <0.01. DEmiRNAs were significantly enriched in 36 pathways (P < 0.05), in which PI3K/Akt signaling was closely related to vascular function. In the validation subjects, the plasma H2S and FMD were higher in the POTS sufferers (P < 0.05).ConclusionElevated whole-blood miR-21 levels serve as an indicator for POTS and may explain the increased plasma H2S observed in POTS sufferers.

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Section: Discussionmentioning

confidence: 99%

Whole-Blood MicroRNA Sequence Profiling and Identification of Specific miR-21 for Adolescents With Postural Tachycardia Syndrome

Lin

Shen²,

Liu³

et al. 2022

Front. Neurosci.

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“…Since miRNA expression is a dynamic process, effective application of the proposed approach for cancer therapy may counterintuitively need to rely on combinations with other therapeutic approaches that upregulate the target oncogenic miRNA. For example, there is evidence that miR-21 is upregulated in response to ionizing radiation (Mahmoudi, et al, 2022). It is conceivable that neoadjuvant application of non-ablative ionizing radiation may synergize with the ss-ppp-miRNA-21 RIG-I agonist to produce improved anti-tumor responses.…”

Section: Discussionmentioning

confidence: 99%

Template-Directed RIG-I Agonist Assembly for Targeted Cancer Immunotherapy

Ghosh¹,

Robertson²,

Crosier³

et al. 2022

Preprint

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Recent developments in the use of pattern recognition receptors (PRRs) aim to harness the innate power of the immune system for cancer therapy. Understanding how to recruit PRRs, such as RIG-I, in a tumor-selective manner is critical for its adoption in the clinic. We describe the use of a tumor-selective template-based agonist of RIG-I to induce type-I IFN signaling and tumor cell apoptosis. The agonist, termed ss-ppp-miRNA-21, comprises a single stranded RNA oligonucleotide modified with a 5-triphosphate and complementary to an endogenous miRNA enriched in tumor cells. We demonstrate the efficacy of the template-directed approach and detail mechanistic studies validating the hypothesis of a template-directed RIG-I agonist assembly using miRNA-21 as a target. The template-directed strategy described here moves us closer to making RIG-I a clinically relevant target in oncology because it achieves targeted activation of innate immunity in the tumor microenvironment in the context of systemic agonist injection.

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“…MicroRNAs (miRNAs) have also been suggested as potential candidates for early AD diagnosis (16). They are a group of non-coding RNAs, containing approximately 22 nucleotides, that regulate target genes (17)(18)(19). The evaluation of expression levels of circulating miRNAs in patients may aid in early AD diagnosis.…”

mentioning

confidence: 99%

MiR-15b and let-7a as Non-invasive Diagnostic Biomarkers of Alzheimer’s Disease Using an Artificial Neural Network

Darvishi Talemi,

Tapak,

Rastgoo Haghi

et al. 2023

Avicenna J Med Biochem

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Background: Gaining insight into the underlying molecular mechanisms of Alzheimer’s disease (AD) is crucial. Objectives: This study aimed to employ a systems biology approach to identify new non-invasive diagnostic biomarkers for AD. Methods: Gene expression data series GSE122063 and microRNA (miRNA) expression data series GSE90828 were obtained from the Gene Expression Omnibus database. The Limma package under R software was used to assess differentially expressed miRNAs and differentially expressed genes (DEGs). Afterward the protein-protein interaction (PPI) network was constructed by the STRING software and evaluated with Cytoscape software. The multilayer perceptron neural network (MLP-NN), a widely used artificial neural network (ANN), was employed to classify two groups. Results: A total of 1388 DEGs were identified in AD patients compared to the control group, and 11 differentially expressed miRNAs were found in patients with mild cognitive impairment (MCI) in comparison to the control group. The results revealed that EGFR, identified as a hub gene, was targeted by miR-15b-3p and let-7a-5p, while TLR4, another hub gene, was targeted by miR-15b-3p. The MLP-NN constructed using both hsa-let-7a-5p and hsa-miR-15b-3p achieved a sensitivity of 0.857 and an area under the curve of 0.917 in detecting Alzheimer’s patients. Conclusion: Our findings suggest that miR-15b-3p, by targeting EGFR and TLR4, and let-7a-5p, by targeting EGFR, may play a significant role in AD. Additionally, the constructed ANN utilizing the expression levels of plasma miR-15b-3p and let-7a-5p could serve as a potential non-invasive diagnostic tool with high sensitivity for AD detection.

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Human exposure to low dose ionizing radiation affects miR-21 and miR-625 expression levels

Cited by 7 publications

References 46 publications

Whole-Blood MicroRNA Sequence Profiling and Identification of Specific miR-21 for Adolescents With Postural Tachycardia Syndrome

Whole-Blood MicroRNA Sequence Profiling and Identification of Specific miR-21 for Adolescents With Postural Tachycardia Syndrome

Template-Directed RIG-I Agonist Assembly for Targeted Cancer Immunotherapy

MiR-15b and let-7a as Non-invasive Diagnostic Biomarkers of Alzheimer’s Disease Using an Artificial Neural Network

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