Human erythrocyte shape regulation: interaction of metabolic and redox status

Truong, Hoai-Thu N.; Daleke, David L.; Huestis, Wray H.

doi:10.1016/0005-2736(93)90120-o

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…Following the retraction of an echinocytogenic stimulus, RBCs can recover from the echinocyte transformation. In some cases, these recovering cells develop a redox-related morphological instability and instead of maintaining the discoid shape, they further proceed to form stomatocytes, in accordance to the degree of the initial crenation [26]. Consequently, it might within the cytosol (cyt, E-G) were also observed.…”

Section: Morphological Analysis Of Pre-hd Rbcs-ros Correlationsmentioning

confidence: 91%

Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis

Antonelou

Kriebardis

Velentzas

et al. 2011

Journal of Proteomics

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Section: Morphological Analysis Of Pre-hd Rbcs-ros Correlationsmentioning

confidence: 91%

Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis

Antonelou

Kriebardis

Velentzas

et al. 2011

Journal of Proteomics

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“…The greatest interest is in RBC samples that are stored for transfusions. The problem with studying RBCs in this environment is that multiple factors are known to affect the RBC shape during the storage period: in addition to the ATP depletion which leads to echinocyte formation [13] (and which is minimized by the SAG-M additives), RBCs in transfusion bags are subjected to pH values that decrease over the storage period [30], which favors stomatocyte formation [31], and increasing amounts of lysolipids in plasma [32] during storage can drive echinocyte formation [33]. The presence of albumin is also a complicating factor: added albumin can cause stomatocyte formation [22,23], because of extraction of lipids from the outer membrane leaflet [34,35]; albumin is also known to interact with DEHP [2].…”

Section: Discussionmentioning

confidence: 99%

The Blood Bag Plasticizer Di-2-Ethylhexylphthalate Causes Red Blood Cells to Form Stomatocytes, Possibly by Inducing Lipid Flip-Flop

Melzak

Uhlig

Kirschhöfer

et al. 2018

Transfus Med Hemother

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Background: During storage of red blood cell (RBC) concentrates, the plasticizer di-2-ethylhexylphthalate (DEHP) that keeps the blood bags soft leaches out and can be taken up by the RBCs. DEHP is known to be beneficial for the RBC storage quality, but the molecular mechanisms of the action are unknown. Methods: Aqueous suspensions of DEHP were added to RBCs in buffer. The morphological effects were observed on RBCs from 5 donors. Flow cytometry with annexin A5 binding was used to measure the exposed phosphatidylserine. Results: DEHP induced the formation of stomatocytes at concentrations as low as ng/ml, provided that the cell suspension was also sufficiently dilute. Some spherocytes, which were susceptible to lysis, were also formed; after lysis, RBC ghosts were seen to continue the transition to the cup-shaped stomatocyte form. Incubation with DEHP increased the exposed phosphatidylserine, an effect that was also observed in the presence of vanadate, which inhibits the ATP-dependent translocases that maintain the membrane's lipid asymmetry. Conclusions: DEHP can have an active effect on RBC shape, instead of just preventing the storage-related shape changes. The effect appears to be mediated by increased flip-flop of lipids between the leaflets of the RBC membrane.

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“…The discocyte‐to‐echinocyte‐to‐spherocyte shape transition of the human erythrocyte (red blood cell; RBC) is emblematic of end‐stage differentiation in cells, so it is notable that under special conditions of incubation the process can be reversed (1). Studying this reversion, and time course kinetics provides a different angle on the understanding of the biochemical and cytoskeletal‐structural mechanisms underlying the discocyte‐to‐echinocyte‐to‐spherocyte shape transition.…”

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confidence: 99%

Erythrocyte shape reversion from echinocytes to discocytes: Kinetics via fast‐measurement NMR diffusion‐diffraction

Pagès

Yau

Kuchel

2010

Magnetic Resonance in Med

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Pulsed field-gradient spin-echo (PGSE) NMR spectroscopy via q-space plots can characterize erythrocyte shapes and their evolution. The present study employed PGSE NMR to investigate shape reversion from advanced echinocytic to normal discocytic shapes due to depletion and then readdition of Mg 21 . In q-space plots of the data, the diffusion-diffraction minima disappeared for Mg 21 -depleted erythrocytes and reappeared during the shape recovery process, but with lower definition than for control cells. Shape estimates from PGSE NMR spectra and light microscopy were in excellent agreement after application of a scaling/correction factor. 31 P NMR was used to probe the biochemical processes activated in erythrocytes after depletion or addition of Mg 21; it showed the activation of the nonoxidative part of the pentose phosphate pathway. Experimental conditions were optimized to bypass this pathway without any influence on the q-space plots. The release of choline from phosphatidylcholine in the outer leaflet of the plasma membrane of the cells, observed using 1 H spin-echo NMR, showed a higher rate for shaperecovered than for control cells. This points to a change in phospholipid asymmetry in the plasma membrane. This variation in asymmetry affected the mean cell shape and hence influenced the average alignment of the erythrocytes with the static magnetic field and so affected the shapes of the qspace plots. Magn Reson Med 64:645-652, 2010. V C 2010 Wiley-Liss, Inc. Key words: 1 H spin-echo NMR; magnesium depletion; phospholipase D; pulsed-field gradient stimulated-echo NMR; q-space imaging; red blood cell shapeThe discocyte-to-echinocyte-to-spherocyte shape transition of the human erythrocyte (red blood cell; RBC) is emblematic of end-stage differentiation in cells, so it is notable that under special conditions of incubation the process can be reversed (1). Studying this reversion, and time course kinetics provides a different angle on the understanding of the biochemical and cytoskeletal-structural mechanisms underlying the discocyte-to-echinocyte-to-spherocyte shape transition. Another type of reversion has recently been reported for human RBCs, whereby membrane flickering that is inhibited with a sulfhydryl reagent can be reinstated with the membrane intercalating compound, diethyl phthalate (2). The physical techniques used in both studies hold promise for a better (quantitative) description of cell shape dynamics.The asymmetric distribution of lipids across the RBC membrane bilayer is an area of high-research activity (3). In normal human RBCs, the membrane bilayer is composed of amino-phospholipids in the inner monolayer (leaflet) while choline-containing phospholipids reside mostly in the outer leaflet (4). Normal RBCs have a characteristic biconcave disk shape. This is a meta-stable shape that involves the continuous adjustment of the asymmetric transbilayer lipid distribution. A change in the lipid location, and so a variation of the membrane asymmetry, appears to be involved in a change in the cell shap...

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Human erythrocyte shape regulation: interaction of metabolic and redox status

Cited by 10 publications

References 33 publications

Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis

Oxidative stress-associated shape transformation and membrane proteome remodeling in erythrocytes of end stage renal disease patients on hemodialysis

The Blood Bag Plasticizer Di-2-Ethylhexylphthalate Causes Red Blood Cells to Form Stomatocytes, Possibly by Inducing Lipid Flip-Flop

Erythrocyte shape reversion from echinocytes to discocytes: Kinetics via fast‐measurement NMR diffusion‐diffraction

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