Human erythrocyte hemolysis induced by selenium and tellurium compounds increased by GSH or glucose: A possible involvement of reactive oxygen species

Schiar, Viviane Patrícia Pires; Santos, Danúbia Bonfanti; Paixão, Márcio W.; Nogueira, Cristina W.; Rocha, João Batista Teixeira da; Zeni, Gilson

doi:10.1016/j.cbi.2008.10.007

Cited by 40 publications

(16 citation statements)

References 35 publications

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“…Specimens were routinely centrifuged within 1 h of collection for 15 min at 2500 g. The supernatant was discarded. The extent of hemolysis was determined as described in [35] with minor modifications. 1 mL of Na + /K + phosphate buffered saline (PBS, pH 7.4) with 6 mM glucose was added to 1 mL of erythrocytes (1×10 9 ).…”

Section: Methodsmentioning

confidence: 99%

Structure-Activity Relationship of Benzophenanthridine Alkaloids from Zanthoxylum rhoifolium Having Antimicrobial Activity

et al. 2014

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Zanthoxylum rhoifolium (Rutaceae) is a plant alkaloid that grows in South America and has been used in Brazilian traditional medicine for the treatment of different health problems. The present study was designed to evaluate the antimicrobial activity of the steam bark crude methanol extract, fractions, and pure alkaloids of Z. rhoifolium. Its stem bark extracts exhibited a broad spectrum of antimicrobial activity, ranging from 12.5 to 100 µg/mL using bioautography method, and from 125 to 500 µg/mL in the microdilution bioassay. From the dichloromethane basic fraction, three furoquinoline alkaloids (1–3), and nine benzophenanthridine alkaloids (4–12) were isolated and the antimicrobial activity of the benzophenanthridine alkaloids is discussed in terms of structure-activity relationships. The alkaloid with the widest spectrum of activity was chelerythrine (10), followed by avicine (12) and dihydrochelerythrine (4). The minimal inhibitory concentrations of chelerythrine, of 1.50 µg/mL for all bacteria tested, and between 3.12 and 6.25 µg/mL for the yeast tested, show this compound to be a more powerful antimicrobial agent when compared with the other active alkaloids isolated from Z. rhoifolium. To verify the potential importance of the methylenedioxy group (ring A) of these alkaloids, chelerythrine was selected to represent the remainder of the benzophenanthridine alkaloids isolated in this work and was subjected to a demethylation reaction giving derivative 14. Compared to chelerythrine, the derivative (14) was less active against the tested bacteria and fungi. Kinetic measurements of the bacteriolytic activities of chelerythrine against the bacteria Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) were determined by optical density based on real time assay, suggesting that its mechanism of action is not bacteriolytic. The present study did not detect hemolytic effects of chelerythrine on erythrocytes and found a protective effect considering the decrease in TBARS and AOPP (advanced oxidized protein products) levels when compared to the control group.

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Section: Methodsmentioning

confidence: 99%

Structure-Activity Relationship of Benzophenanthridine Alkaloids from Zanthoxylum rhoifolium Having Antimicrobial Activity

et al. 2014

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“…Tungsten (W), is a transition metal used extensively for Wlaments for electric lamps, and television tubes with no recognized physiologic role and very limited available information of its toxicological eVects (Goldoni et al 2004;Koutsospyros et al 2006;Strigul et al 2010), although, in recent years W compounds have been proposed as anti-diabetic and anti-obesity agents (BarceloBatllori et al 2005;Canals et al 2009). Tellurium (Te) is used in semiconductor device fabrication (Schiar et al 2009), and can inhibit the activity of selenium dependent glutathione peroxidase, which can lead to an exacerbation of intracellular oxidative stress (Garberg et al 1999). Beryllium (Be), is used as a material in high-technology industries, such as aerospace, nuclear defense, and electronics.…”

Section: Introductionmentioning

confidence: 99%

Comparison of urine toxic metals concentrations in athletes and in sedentary subjects living in the same area of Extremadura (Spain)

et al. 2011

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Cadmium (Cd), tungsten (W), tellurium (Te), beryllium (Be), and lead (Pb), are non-essential metals pervasive in the human environment. Studies on athletes during training periods compared to non-training control subjects, indicate increased loss of minerals through sweat and urine. The aim of this study was to compare the level of these trace elements, determined by inductively coupled plasma mass spectrometry (ICP-MS) in urine samples, between athletes and age-matched sedentary subjects living in the same geographical area, although anthropometric and cardiovascular measurements showed that athletes have significantly (P ≤ 0.001) lower BMI, body fat and heart rate, whereas the muscle and bone percentage was significantly (P ≤ 0.001) higher than in sedentary subjects. The validity of the methodology was checked by the biological certified reference material. Trace element analysis concentrations, expressed in μg/mg creatinine, of five toxic elements in urine from athletes (n = 21) versus sedentary subjects, (n = 26) were as follows: Cd (0.123 ± 0.075 vs. 0.069 ± 0.041, P ≤ 0.05); W (0.082 ± 0.053 vs. < limit of detection); Te (0.244 ± 0.193 vs. 0.066 ± 0.045, P ≤ 0.001), Be (0.536 ± 0.244 vs. 0.066 ± 0.035, P ≤ 0.001); Pb (0.938 ± 0.664 vs. 2.162 ± 1.444 P ≤ 0.001). With the exception of Pb, urine toxic metal concentrations from athletes were higher than from sedentary subjects. This fact suggests that physical activity counteracts, at least in part, the cumulative effect of toxic environment by increasing the urine excretion of toxic metals in trained people.

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“…DPDT can also be teratogenic, causing various morphologic abnormalities in mice foetuses during development [12]. In addition, it produces renal and hepatic toxicity in rodents as well as haematological disorders in human beings [7,13]. In contrast to these toxic effects, studies have demonstrated that DPDT in low concentrations is able to prevent oxidative stress induced by several oxidizing agents [14].…”

mentioning

confidence: 96%

Diphenyl Ditelluride‐Induced Cell Cycle Arrest and Apoptosis: A Relation with Topoisomerase I Inhibition

Jorge

Oliveira

Filippi-Chiela

et al. 2014

Basic Clin Pharma Tox

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The diphenyl ditelluride (DPDT) is a prototype for the development of new biologically active molecules. In previous studies, DPDT showed an elevated cytotoxicity in Chinese hamster fibroblast (V79) cells but the mechanisms for reduction of cell viability still remain unknown. DPDT showed mutagenic properties by induction of frameshift mutations in bacterium Salmonella typhimurium and yeast Saccharomyces cerevisiae. This organotelluride also induced DNA strand breaks in V79 cells. In this work, we investigated the mechanism of DPDT cytotoxicity by evaluating the effects of this compound on cell cycle progression, apoptosis induction and topoisomerase I inhibition. Significant decrease of V79 cell viability after DPDT treatment was revealed by MTT assay. Morphological analysis showed induction of apoptosis and necrosis by DPDT in V79 cells. An increase of caspase 3/7 activity confirmed apoptosis induction. The cell cycle analysis showed an increase in the percentage of V79 cells in S phase and sub-G1 phase. The yeast strain deficient in topoisomerase I (Topo I) showed higher tolerance to DPDT compared with the isogenic wild-type strain, suggesting that the interaction with this enzyme could be involved in DPDT toxicity. The sensitivity to DPDT found in top3Δ strain indicates that yeast topoisomerase 3 (Top3p) could participate in the repair of DNA lesions induced by the DPDT. We also demonstrated that DPDT inhibits human DNA topoisomerase I (Topo I) activity by DNA relaxation assay. Therefore, our results suggest that the DPDT-induced cell cycle arrest and reduction in cell viability could be attributed to interaction with topoisomerase I enzyme.

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Human erythrocyte hemolysis induced by selenium and tellurium compounds increased by GSH or glucose: A possible involvement of reactive oxygen species

Cited by 40 publications

References 35 publications

Structure-Activity Relationship of Benzophenanthridine Alkaloids from Zanthoxylum rhoifolium Having Antimicrobial Activity

Structure-Activity Relationship of Benzophenanthridine Alkaloids from Zanthoxylum rhoifolium Having Antimicrobial Activity

Comparison of urine toxic metals concentrations in athletes and in sedentary subjects living in the same area of Extremadura (Spain)

Diphenyl Ditelluride‐Induced Cell Cycle Arrest and Apoptosis: A Relation with Topoisomerase I Inhibition

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