Human epicardium-derived cells fuse with high efficiency with skeletal myotubes and differentiate toward the skeletal muscle phenotype: a comparison study with stromal and endothelial cells

Gentile, Antonietta; Toietta, Gabriele; Pazzano, Vincenzo; Tsiopoulos, Vasileios; Giglio, Ada Francesca; Crea, Filippo; Pompilio, Giulio; Capogrossi, Maurizio C.; Rocco, Giuliana Di

doi:10.1091/mbc.e10-06-0537

Cited by 5 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was shown initially that human MSCs derived from bone marrow were capable of spontaneous fusion with murine C2C12 myoblasts 14 and later that such fusion led to expression of human proteins characteristic of skeletal muscle indicating that the MSC nuclei were transcriptionally active post-fusion. 15 , 16 Similar results have also been demonstrated using MSCs derived from adipose tissue. 17 …”

Section: Introductionsupporting

confidence: 67%

Uniaxial cyclic strain of human adipose–derived mesenchymal stem cells and C2C12 myoblasts in coculture

2014

View full text Add to dashboard Cite

Tissue engineering skeletal muscle in vitro is of great importance for the production of tissue-like constructs for treating tissue loss due to traumatic injury or surgery. However, it is essential to find new sources of cells for muscle engineering as efficient in vitro expansion and culture of primary myoblasts are problematic. Mesenchymal stem cells may be a promising source of myogenic progenitor cells and may be harvested in large numbers from adipose tissue. As skeletal muscle is a mechanically dynamic tissue, we have investigated the effect of cyclic mechanical strain on the myogenic differentiation of a coculture system of murine C2C12 myoblasts and human adipose–derived mesenchymal stem cells. Fusion of mesenchymal stem cells with nascent myotubes and expression of human sarcomeric proteins was observed, indicating the potential for myogenic differentiation of human mesenchymal stem cells. Cyclic mechanical strain did not affect the fusion of mesenchymal stem cells, but maturation of myotubes was perturbed.

show abstract

Section: Introductionsupporting

confidence: 67%

Uniaxial cyclic strain of human adipose–derived mesenchymal stem cells and C2C12 myoblasts in coculture

2014

View full text Add to dashboard Cite

show abstract

“…However, blocking VEGF induced an increase in myotube formation in all conditions, suggesting that the effect of VEGF on MPCs is mainly on cell growth. IL‐13 regulates the secondary recruitment of reserve cells into myotubes, particularly on IGF‐1‐stimulated human cells [68] and increases fusion into myotubes [69]. Unexpectedly, blocking IL‐13 induced increased myotube formation in MPCs cultured with M1 MP‐conditioned medium, suggesting that IL‐13 participates in the nondifferentiating property of M1 MPs.…”

Section: Discussionmentioning

confidence: 99%

Differentially Activated Macrophages Orchestrate Myogenic Precursor Cell Fate During Human Skeletal Muscle Regeneration

et al. 2013

View full text Add to dashboard Cite

Macrophages (MPs) exert either beneficial or deleterious effects on tissue repair, depending on their activation/ polarization state. They are crucial for adult skeletal muscle repair, notably by acting on myogenic precursor cells. However, these interactions have not been fully characterized. Here, we explored both in vitro and in vivo, in human, the interactions of differentially activated MPs with myogenic precursor cells (MPCs) during adult myogenesis and skeletal muscle regeneration. We showed in vitro that through the differential secretion of cytokines and growth factors, proinflammatory MPs inhibited MPC fusion while anti-inflammatory MPs strongly promoted MPC differentiation by increasing their commitment into differentiated myocytes and the formation of mature myotubes. Furthermore, the in vivo time course of expression of myogenic and MP markers was studied in regenerating human healthy muscle after damage. We observed that regenerating areas containing proliferating MPCs were preferentially associated with MPs expressing proinflammatory markers. In the same muscle, regenerating areas containing differentiating myogenin-positive MPCs were preferentially coupled to MPs harboring anti-inflammatory markers. These data demonstrate for the first time in human that MPs sequentially orchestrate adult myogenesis during regeneration of damaged skeletal muscle. These results support the emerging concept that inflammation, through MP activation, controls stem cell fate and coordinates tissue repair. STEM CELLS 2013;31:384-396 Disclosure of potential conflicts of interest is found at the end of this article.

show abstract

“…Another possible explanation for the seemingly low fusion index with HPPs and myotubes is that the HPPs were derived from human cells, and the myotubes were generated from mouse cells. However, Gentile et al recently reported that human epicardium‐derived cells fused to mouse myoblasts with high efficiency (12%) when co‐cultured at similar ratios to the one used in the current study. In contrast, the same study reported that human adipose tissue‐derived mesenchymal stromal cells fused less efficiently than endocardial cells (<1%), suggesting that cell type, rather than species difference has a greater impact on fusion potential.…”

Section: Discussionmentioning

confidence: 52%

NF‐KB activity functions in primary pericytes in a cell‐ and non‐cell‐autonomous manner to affect myotube formation

2013

View full text Add to dashboard Cite

show abstract

Human epicardium-derived cells fuse with high efficiency with skeletal myotubes and differentiate toward the skeletal muscle phenotype: a comparison study with stromal and endothelial cells

Cited by 5 publications

References 41 publications

Uniaxial cyclic strain of human adipose–derived mesenchymal stem cells and C2C12 myoblasts in coculture

Uniaxial cyclic strain of human adipose–derived mesenchymal stem cells and C2C12 myoblasts in coculture

Differentially Activated Macrophages Orchestrate Myogenic Precursor Cell Fate During Human Skeletal Muscle Regeneration

NF‐KB activity functions in primary pericytes in a cell‐ and non‐cell‐autonomous manner to affect myotube formation

Contact Info

Product

Resources

About