Human endothelial cells are chemotactic to endothelial cell growth factor and heparin.

Abstract

The response of human endothelial cell migration to various extracellular matrix components and growth factors has been assessed. Human endothelial cells demonstrate increased chemotaxis and chemokinesis when placed in a modified Boyden chamber with endothelial cell growth factor (ECGF) used at a concentration of 10 -9 M. Anti-ECGF antibody inhibits the chemotactic response. Heparin (10 -a to 10 -l° M) was also chemotactic and was shown to potentiate the chemotactic activity of ECGF. Although laminin, fibronectin, the polypeptide (epidermal, fibroblast, and nerve) growth factors, and collagen types I, II, Iit, IV, and V demonstrate a chemotactic response, these activities were one third to one half less than observed with ECGF. These data suggest that ECGF and heparin may play a significant role as response modifiers of human endothelial cell migration which may be relevant to tumor metastasis, wound healing, and atherogenesis.

Fibroblast growth factor/fibroblast growth factor receptor system in angiogenesis

“…FGF1 and FGF2 [27,28], the FGF8b isoform [17], and FGF10 [29] stimulate chemotaxis and/or chemokinesis in endothelial cells. Similar to cell proliferation, the chemotactic effect of FGF2 requires the activation of the MAPK signaling pathway [30] and is abolished by PKC down-regulation [31].…”

Formação in vitro de túbulos capilares a partir de células de sangue de cordão umbilical humano com perspectivas para aplicação terapêutica

“…The capacity of cell migration is essential for forming new vessels and capillaries, and it is a characteristic of ECs that are capable of an organization resulting in formation of three-dimensional in-vitro tubular structures [21].…”

Effect of Octreotide on experimental corneal neovascularization

“…Growth factors which are mitogenic proteins take part in tissue repair processes (Leibowitz et al 1990;Seymor 1985), however, they stimulate neovascularization during this repairing process (Scott et al 1995;Terranova et al 1985). Epidermal growth factor (EGF) has been known to induce neovascularization by increasing the vascular endothelial mitotic activity and plasminogen activator release besides increasing the fibronectin, collagen and proteoglycan synthesis in extracellular matrix (Leibowitz et al 1990;Seymor 1985).…”