Human embryos secrete microRNAs into culture media—a potential biomarker for implantation

Rosenbluth, Evan M.; Shelton, Dawne N.; Wells, Lindsay M.; Sparks, Amy E.T.; Voorhis, Bradley J. Van

doi:10.1016/j.fertnstert.2014.01.058

Cited by 180 publications

(223 citation statements)

References 22 publications

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“…MSC-derived exosomal microvesicles have been recently documented and may provide a potential explanation for the micromodulation potential observed with stem cells. Moreover, it was recently shown that human and cattle preimplantation embryos also secreted exosomes throughout the in vitro culture period [31,32]. Therefore, one may also speculate a potential reciprocal effect between b-ATMSCs and embryos in culture that may explain the observed effects.…”

Section: Discussionmentioning

confidence: 96%

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Miranda

Nascimento

Costa

et al. 2016

J Assist Reprod Genet

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Purpose Despite advances in the composition of defined embryo culture media, co-culture with somatic cells is still used for bovine in vitro embryo production (IVEP) in many laboratories worldwide. Granulosa cells are most often used for this purpose, although recent work suggests that co-culture with stem cells of adult or embryonic origin or their derived biomaterials may improve mouse, cattle, and pig embryo development. Materials and methods In experiment 1, in vitro produced bovine embryos were co-cultured in the presence of two concentrations of bovine adipose tissue-derived mesenchymal cells (b-ATMSCs; 10 3 and 10 4 cells/mL), in b-ATMSC preconditioned medium (SOF-Cond), or SOF alone (control). In experiment 2, co-culture with 10 4 b-ATMSCs/mL was compared to the traditional granulosa cell co-culture system (Gran). Results In experiment 1, co-culture with 10 4 b-ATMSCs/mL improved blastocyst rates in comparison to conditioned and control media (p < 0.05). Despite that it did not show difference with 10 3 b-ATMSCs/mL (p = 0.051), group 10 4 bATMSCs/mL yielded higher results of blastocyst production.In experiment 2, when compared to group Gran, co-culture with 10 4 b-ATMSCs/mL improved not only blastocyst rates but also quality as assessed by increased total cell numbers and mRNA expression levels for POU5F1 and G6PDH (p < 0.05). Conclusions Co-culture of bovine embryos with b-ATMSCs was more beneficial than the traditional co-culture system with granulosa cells. We speculate that the microenvironmental modulatory potential of MSCs, by means of soluble substances and exosome secretions, could be responsible for the positive effects observed. Further experiments must be done to evaluate if this beneficial effect in vitro also translates to an increase in offspring following embryo transfer. Moreover, this study provides an interesting platform to study the basic requirements during preimplantation embryo development, which, in turn, may aid the improvement of embryo culture protocols in bovine and other species.

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Section: Discussionmentioning

confidence: 96%

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Miranda

Nascimento

Costa

et al. 2016

J Assist Reprod Genet

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“…Initial studies have shown that the types of miRNAs secreted into the culture media are differentially expressed depending on the method of fertilization (ICSI or IVF) and may correlate with pregnancy outcomes. These data suggest that miRNAs within the secretome of cleavage stage embryos may serve as potential biomarkers of embryo developmental potential (Rosenbluth et al 2014). The detection of RNAs from polar body biopsy of oocytes and/or embryonic secretome during culture may be used in the future to select oocytes and/or embryos with the highest developmental potential.…”

Section: Assisted Reproductive Technologiesmentioning

confidence: 95%

MicroRNA in Ovarian Biology and Disease

McGinnis

Luense

Christenson

2015

Cold Spring Harb Perspect Med

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“…Aberrant miRNA profiles in transferable blastocysts have been linked with male factor infertility and polycystic ovaries [5]. Since miRNAs are known to be secreted [18], attempts have been made to link specific miRNAs detected in the SBM with embryo ploidy status and reproductive competence [2][3][4][5]. miRNAs detected in the spent medium from early embryos might be the output of paracrine circuits, reflecting normal communication among cells of healthy embryos; alternatively, they might be a component of the communication pathway between the embryo and the uterine epithelium, influencing epithelial readiness for embryo apposition and implantation.…”

Section: Microrna Profile In Sbms Is Similar Among Twins a And B Butmentioning

confidence: 99%

“…They are secreted and can be found in embryo culture media. Attempts have been made to link specific miRNAs detected in the medium with embryo ploidy status and reproductive competence to predict in vitro fertilization (IVF) outcome, and several candidates have been identified [2][3][4][5]. Although further studies are warranted, a comprehensive profile of miRNAs secreted by human preimplantation embryos in spent culture media is taking shape [6].…”

Section: Introductionmentioning

confidence: 99%

Human Embryos Created by Embryo Splitting Secrete Significantly Lower Levels of miRNA-30c

Noli

Capalbo

Dajani

et al. 2016

Stem Cells and Development

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Studies reporting term pregnancy and the production of genetically identical offspring from isolated blastomeres of early stage embryos have been carried out in small and large animals. However, very little is known about the effects of embryo splitting on the development and reproductive competency of human embryos. In this study, we investigated the effects of embryo splitting on profile of microRNAs (miRNAs) detected in their spent blastocyst medium (SBM) by comparative analysis of miRNA profiles in SBM of human twin embryos created by blastomere biopsy and SBM of blastocysts that resulted in a healthy pregnancy and live birth following embryo transfer. The profile of miRNA secretion in in vitro culture media consistently distinguishes twin from control embryos. We found that six miRNAs are significantly more abundant in SBM from twin embryos, while nine are significantly more abundant in SBM from euploid implanted blastocysts. These nine include miRNA-30c, a previously reported marker of blastocyst implantation potential. Furthermore, 22.9% of miRNAs secreted by twin embryos were never detected in SBM from normal reproductively competent blastocysts, or from trophectoderm (TE) samples from normal blastocysts donated for the research. The miRNA profile, unique to twin blastocysts, might be a result of differential lineage commitment in these embryos.

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Human embryos secrete microRNAs into culture media—a potential biomarker for implantation

Cited by 180 publications

References 22 publications

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

Increasing of blastocyst rate and gene expression in co-culture of bovine embryos with adult adipose tissue-derived mesenchymal stem cells

MicroRNA in Ovarian Biology and Disease

Human Embryos Created by Embryo Splitting Secrete Significantly Lower Levels of miRNA-30c

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