Human embryos arrest in a quiescent-like state characterized by metabolic and zygotic genome activation problems

Yang, Yang; Shi, Lina; Fu, Xiaozhe; Ma, Gang; Yang, Zhongzhou; Li, Yuhao; Zhou, Yibin; Yuan, Lihua; Xia, Ye; Zhong, Xiufang; Yin, Ping; Sun, Li; Zhang, Wuwen; Babarinde, Isaac A.; Wang, Yongjun; Zhao, Xiaoyang; Hutchins, Andrew P.; Tong, Guoqing

doi:10.1101/2021.12.19.473390

Cited by 2 publications

(1 citation statement)

References 100 publications

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“…The use of an albumin-free medium can increase the number of identified proteins, as shown in a study on the secretome of murine embryos [ 23 ]. Additionally, the substantial number of arrested and apoptotic cells in in-vitro-produced embryos should be considered [ 43 , 44 , 45 ], since their cell membranes may be damaged, which can cause a passive leakage of proteins to the culture medium [ 46 ]. Since this could mask actively secreted autocrine factors, individual culture is required, and the ratio of membrane-damaged cells (MDCs) should be taken into consideration as a quality parameter when secretome analysis is performed.…”

Section: Introductionmentioning

confidence: 99%

Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

Raes

Wydooghe

Pavani

et al. 2023

IJMS

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While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. We hypothesize that supplementation of embryotrophic proteins to a culture medium could be the key to improve individual embryo production. In this study, proteomics screening of culture media conditioned by bovine embryos revealed cathepsin-L as being secreted by both excellent- and good-quality embryos, while being absent in the medium conditioned by poor-quality embryos. The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. Preliminary dose–response experiments pointed out 100 ng/mL as the optimal concentration of cathepsin-L in embryo culture medium. Supplementation of cathepsin-L to individual culture systems significantly improved blastocyst development and quality in terms of blastocoel formation at day 7, and the hatching ratio and apoptotic cell ratio at day 8, compared to the control. Taken together, cathepsin-L acts as an important embryotrophin by increasing embryo quality, and regulating blastulation and hatching in bovine in vitro embryo production.

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Section: Introductionmentioning

confidence: 99%

Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

Raes

Wydooghe

Pavani

et al. 2023

IJMS

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Evaluation of the causes of pre-implantation developmental arrest and ways to overcome it

Karami,

Hassani,

Eftekhari-Yazdi

et al. 2023

jct

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In this review article, we intend to investigate and review the factors that lead to embryonic developmental arrest and explore different strategies to address this phenomenon. We aim to pave the way for further research in this field and enhance the efficiency of the IVF method for infertility treatment.Introduction: To ensure proper embryonic development, embryos need to progress through their developmental stages unimpeded. Any disruptions in these stages including oocyte factors, sperm factors, and embryonic factors can result in infertility due to pre-implantation developmental arrest. Therefore, it can be very helpful to understand the effective factors behind this phenomenon.Topic: Among couples attempting to conceive, infertility persists despite their best efforts. Couples are typically considered infertile if they have tried to conceive for twelve months without success and without using protection; but if the woman is over 37 years old, this twelve months will be reduced to six months. Assisted reproductive techniques (ART) encompass various methods that are aimed at aiding these couples in overcoming infertility. This definition includes any manipulation of embryos and eggs for infertility treatment. Assisted reproductive techniques include in vitro maturation (IVM), in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI). Among these, IVF/ICSI is particularly effective in treating couples infertility. However, one common challenge in this process is embryonic developmental arrest during the pre-implantation stages, a primary cause of infertility in treatment cycles. Approximately 40 to 50 percent of IVF cycles do not progress to the blastocyst stage and arrest. This arrest characterized by a lack of cell division for at least 24 hours. Most embryonic arrests occur on the second and third day after fertilization, during the two to eight-cell stages. These arrested embryos retain their developmental potential, and their gene expression program related to their arrested stage remains

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Human embryos arrest in a quiescent-like state characterized by metabolic and zygotic genome activation problems

Cited by 2 publications

References 100 publications

Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect In Vitro

Evaluation of the causes of pre-implantation developmental arrest and ways to overcome it

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