Human Embryonic Stem Cell Lines Generated without Embryo Destruction

Chung, Young Sun; Klimanskaya, Irina; Becker, Sandy; Li, Tong; Maserati, Marc Peter; Lu, Shi‐Jiang; Zdravkovic, Tamara; Ilić, Duško; Genbačev, Olga; Fisher, Susan J.; Krtolica, Ana; Lanza, Robert

doi:10.1016/j.stem.2007.12.013

Cited by 210 publications

(142 citation statements)

References 20 publications

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“…However, most of the latest articles tend to report only optimizations of the derivation protocols (e.g. [16,17]) or hESC lines established from alternative sources of embryo, for example from poor quality embryos or morulae [18][19][20]. In addition, in the most recent papers, the report on the derivation of the new hESC lines usually accounted for a small part of these publications, where the cell line served as the basis for the development of the main scientific study, indicating less availability for publishing new derivations without any novel scientific aspect.…”

Section: Gathering Information From Human Embryonic Stem Cell Registriesmentioning

confidence: 99%

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

Fraga¹,

Araújo²,

Stabellini

et al. 2011

Stem Cell Rev and Rep

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Since the derivation of the first human embryonic stem cell (hESC) lines by Thomson and coworkers in 1998, more than 1,200 different hESC lines have been established worldwide. Nevertheless, there is still a recognized interest in the establishment of new lines of hESC, particularly from HLA types and ethnic groups currently underrepresented among the available lines. The methodology of hESC derivation has evolved significantly since 1998, when human LIF (hLIF) was used for maintenance of pluripotency. However, there are a number of different strategies for the several steps involved in establishing a new line of hESC. Here we make a survey of the most relevant parameters used between 1998 and 2010 for the derivation of the 375 hESC lines deposited in two international stem cell registries, and able to form teratomas in immunocompromised mice. Although we identify some trends in the methodology for establishing hESC lines, our data reveal a much greater heterogeneity of strategies than what is used for derivation of murine ESC lines, indicating that optimum conditions have not been consolidated yet, and thus, hESC establishment is still an evolving field of research.

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Section: Gathering Information From Human Embryonic Stem Cell Registriesmentioning

confidence: 99%

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

Fraga¹,

Araújo²,

Stabellini

et al. 2011

Stem Cell Rev and Rep

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“…Moreover, expression of tissue inhibitor of metalloproteinase-4 (TIMP4) in differentiating cells needs Matrix metalloproteinase-9 (MMP9) that induces fibrosis and causes stiffness of the ECM and impairs differentiation of cardiac stem cells into cardiomyocytes (Figure 6b) [65]. However, for clinical use various embryonic stem cell lines [73] have been derived from human blastocysts without destruction of human embryo [74,75]. These cell lines require expression of several transcription factors and cell surface proteins for doi: 10.7243/2050-1218-4-1 differentiation.…”

Section: Embryonic Stem Cellsmentioning

confidence: 99%

“…No doubt SFAE put beneficial influence on adipogenesis and angiogenesis both in vitro and in vivo [157]. Therefore, for harvesting adipose-derived stromal cells (ASCs) suction-assisted lipoaspirate liposuction is used for tissue engineering to avoid negative effect on ASCs [74].…”

Section: Repairing Of Adipose Tissue Damagementioning

confidence: 99%

Role of regeneration in tissue repairing and therapies

Upadhyay¹

2015

J Regen Med Tissue Eng

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Present review article emphasizes role of regeneration mechanism in various tissues and its use in organ transplantation for wound healing and repairing. This article also explains uses of various stem cell types in transplantation technologies and its applications in regenerative medicine and therapeutics. This review also addresses tissue engineering methods and use of new biological scaffold materials and promising candidates such as immunomodulators, adhesions, integrins in tissue repairing and induction of regeneration in injured tissues. In addition, role of various molecules of immune cell system, gene cascades and transcription specific proteins (TSPs) and growth factors in formation of microenvironment for differentiation are also explained. Moreover, mechanism of regeneration in various tissues such as neural, skeletal, cardiac, muscular, adipose and gonadial tissues, and bone marrow is described in detail. This article also suggest an urgent need for development of new advanced methods, technologies, biomatrices, polymers and scaffold materials, and cell based therapies to overcome the problem of disable and injured patients. Hence, landmark innovations are required in the field of regenerative medicine, tissue engineering, and developmental biology for successful tissue repairing and organ transplants to serve the human society.

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“…On the other hand, different groups have tried to derive ESCs from isolated blastomeres [10][11][12][13][14]. The production of ESC lines from isolated blastomeres arose as an answer to the ethic and moral debate about the use of whole embryos for ESC derivation and it is based on the preimplantation genetic diagnosis (PGD) experience.…”

Section: Introductionmentioning

confidence: 99%

“…This method provided higher efficiencies of derivation than the method of aggregation with proven ESCs-GFP. Recently, Chung et al (2008) [11] have found that the derivation efficiency of human ESC lines established from 8-cell stage blastomeres can be improved by culturing them in a medium containing laminin and fibronectin, a new approach that recreates the ICM niche. By contrast, Geens et al (2009) [12] described the derivation of human ESC lines from isolated blastomeres of 4-cell stage embryos by simply culturing blastomeres onto a monolayer of feeder cells.…”

Section: Introductionmentioning

confidence: 99%

Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods

González

Ibáñez

Santaló

2010

J Assist Reprod Genet

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Purpose The aim of the present study is to compare three previously described mouse embryonic stem cell derivation methods to evaluate the influence of culture conditions, number of isolated blastomeres and embryonic stage in the derivation process. Methods Three embryonic stem cell derivation methods: standard, pre-adhesion and defined culture medium method, were compared in the derivation from isolated blastomeres and whole embryos at 4-and 8-cell stages. Results A total of 200 embryonic stem cell lines were obtained with an efficiency ranging from 1.9% to 72%. Conclusions Using either isolated blastomeres or whole embryos, the highest rates of mouse embryonic stem cell establishment were achieved with the defined culture medium method and efficiencies increased as development progressed. Using isolated blastomeres, efficiencies increased in parallel to the proportion of the embryo volume used to start the derivation process.

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Human Embryonic Stem Cell Lines Generated without Embryo Destruction

Cited by 210 publications

References 20 publications

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

A Survey of Parameters Involved in the Establishment of New Lines of Human Embryonic Stem Cells

Role of regeneration in tissue repairing and therapies

Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods

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