Human Embryonic Prealbumin as a Marker for Tumours and Fibroblasts

Tatarinov, YuS.; Vasiliev, M.Yu.; Kalashnikov, V. V.; Voloshuk, S. G.; Kraevsky, N. A.; Sg, Vorsanova

doi:10.1016/s0140-6736(78)92276-6

Cited by 12 publications

(3 citation statements)

References 7 publications

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“…The activated product had an Rrof0 .80 in the solvent described compared with 0.25 for free daunorubicin. The coefficient of extinction E (24,25) in desmoid tumours and soft tissue cancers. Embryonic prealbumin was prepared according to Drouin (26) and 250 ~g were inoculated intradermally into Balb-C mice with complete Freund's adjuvant.…”

Section: T He Use Of Cytotoxic Drugs For the Treatment Ofmentioning

confidence: 99%

Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method

Page¹,

Thibeault²,

Tremblay³

et al. 1992

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Conjugation of daunorubicin to monoclonal antihuman sarcoma antibody by a novel method. Can J Infect Dis 1992;3(Suppl B): 101B-105B. Most of the reported methods for coupling anthracycline drugs to antibody result in either a loss of pharmacological activity or yield antibodies which have lost much of their immunological specificity. The use of glutaraldehyde for coupling saves both the pharmacological and immunological activities but it causes some polymerization of the antibody or of the macromolecular carrier which is undesirable for in vivo use. A new coupling procedure is reported which uses an activated derivative of daunorubicin added to monoclonal antihuman sarcoma antibody. This coupling procedure has not resulted in significant polymerization of the antibody or Joss of ph armacological activity determined by testing normal and tumour cell L ines in vitro.

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Section: T He Use Of Cytotoxic Drugs For the Treatment Ofmentioning

confidence: 99%

Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method

Page¹,

Thibeault²,

Tremblay³

et al. 1992

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…The striking outcome of these studies was uncovering progressive accumulation of chromosomal instability (suggested aneuploidization) in senescent cells or aged cell populations proposing a new cytogenetic paradigm for aging research [ 4 , 5 ]. Although these findings and idea was dogmatically misunderstood, open-minded researchers found these data applicable for discovering biomarkers of cancer [ 6 ]. Now we know that these findings (discoveries) are valid, inasmuch as chromosome instability and aneuploidization are confirmed biomarkers of cellular aging and senescence.…”

mentioning

confidence: 99%

Svetlana G. Vorsanova (1945–2021)

Iourov

2022

Mol Cytogenet

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“…Immunodiffusion studies have also demonstrated that it is present in homogenates of tumours, especially those of connective tissue origin, and in over 17 different fetal adult fibroblast cell lines. [2][3][4] With the fluorescent antibody technique Kalashnikov and his colleagues3 have shown it can be localised within placenta, umbilical cord, fetal tissues (including skin, bone, and kidney) and in the stroma surrounding some epithelial tumours. In our study, using an indirect immunoperoxidase technique, we have examined formalin-fixed paraffin embedded human fetal and adult tissues for the presence and distribution of EPA, including benign and malignant tumours.…”

mentioning

confidence: 99%

Localisation of embryonic prealbumin in formalin-fixed human fetal and adult tissue.

Gallon¹,

Reid²,

McHardie³

et al. 1981

J Clin Pathol

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Accepted for publication 26 November 1980 Material and methods Paraffin-embedded blocks of human tissues which had been fixed in either formol saline or neutral buffered formalin and stored for up to four years, were obtained from the files of the pathology departments in Aberdeen and Glasgow. The EPA antiserum (provided by Professor Tatarinov) was raised in rabbits using semi-purified EPA preparations and rendered monospecific by subsequent absorption using pooled human plasma and extract of normal tissues. IMMUNOPEROXIDASE TECHNIQUEThe immunoperoxidase techniques used in both departments were essentially the same except that the addition of trypsin before the addition of the normal swine serum was not carried out by the Glasgow department. Despite this there did not appear to be any qualitative difference in the preparations from the two departments.Sections (5 ,tm) were made from paraffin-embedded blocks of tissue. After taking the sections to water endogenous peroxidase was blocked by treating the sections with 05 Y% H202 in methanol for 30 min5 and the sections were then washed in distilled water.

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Human Embryonic Prealbumin as a Marker for Tumours and Fibroblasts

Cited by 12 publications

References 7 publications

Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method

Conjugation of Daunorubicin to Monoclonal Antihuman Sarcoma Antibody by a Novel Method

Svetlana G. Vorsanova (1945–2021)

Localisation of embryonic prealbumin in formalin-fixed human fetal and adult tissue.

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