Human Elastin‐Based Recombinant Biopolymers Improve Mesenchymal Stem Cell Differentiation

Çelebi, Betül; Cloutier, Maxime; Rabelo, Rodrigo Balloni; Bandiera, Antonella

doi:10.1002/mabi.201200170

Cited by 25 publications

(23 citation statements)

References 50 publications

(56 reference statements)

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“…Additionally, the same study found ELP coatings on plastic and glass supported osteogenic differentiation of MSCs and high cell viability even in the absence of osteogenic media 108 . The surfaces of ELP based materials can be tuned at a nanometer scale to explore the in-depth effects of topography on cell behavior.…”

Section: Applications Of Mscs In Combination With Elastin-based Bimentioning

confidence: 76%

“…Recombinant ELPs modified with extra cross-linking sites to promote fibril formation have been demonstrated to enhance human bmMSC adhesion and proliferation in comparison to purely hydrophobic ELPs 108 . Additionally, the same study found ELP coatings on plastic and glass supported osteogenic differentiation of MSCs and high cell viability even in the absence of osteogenic media 108 .…”

Section: Applications Of Mscs In Combination With Elastin-based Bimentioning

confidence: 99%

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Elastin-based biomaterials and mesenchymal stem cells

et al. 2015

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Elastin is the dominant mammalian elastic protein found in soft tissue. Elastin-based biomaterials have the potential to repair elastic tissues by improving local elasticity and providing appropriate cellular interactions and signaling. Studies that combine these biomaterials with mesenchymal stem cells have demonstrated their capacity to also regenerate non-elastic tissue. Mesenchymal stem cell differentiation can be controlled by their immediate environment, and their sensitivity to elasticity makes them an ideal candidate for combining with elastin-based biomaterials. With the growing accessibility of the elastin precursor, tropoelastin, and elastin-derived materials, the amount of research interest in combining these two fields has increased and, subsequently, is leading to the realization of a potentially new strategy for regenerative medicine.

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Section: Applications Of Mscs In Combination With Elastin-based Bimentioning

confidence: 76%

Section: Applications Of Mscs In Combination With Elastin-based Bimentioning

confidence: 99%

Elastin-based biomaterials and mesenchymal stem cells

et al. 2015

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“…Elastic protein (elastin) is a core protein of elastic fiber located in the pelvic floor extracellular matrix (ECM) [13]. Reduced synthesis and changes in the structure of elastin can promote the occurrence of POP.…”

Section: Wwwimpactjournalscom/oncotarget/ Oncotarget Advance Publimentioning

confidence: 99%

Mechanical stretching promotes the differentiation of BMSC into pelvic floor ligament fibroblasts

Zhao¹,

Wang²,

Ren³

et al. 2018

Oncotarget

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Pelvic floor dysfunction (PFD) is a prevalent and debilitating condition in agingwomen that is associated with weakened pelvic connective tissue. Fibroblasts are the main component of pelvic connective tissue and play a vital role in the maintenance of the pelvic organ. Mechanical stretching is a known modulator leading to the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). In this paper, we measured the lysyl oxidase (LOX), elastin and KDM4B expression in cultured BMSCs under different cyclic mechanical stretch (CMS) conditions via Western blot assays. Our findings indicated that the expression of these proteins was significantly higher in the CMS group than in the normal control group, and 10% CMS at a 0.5 Hz frequency had the best stimulatory effect. We further evaluated the influence of KDM4B on LOX and elastin expression via knockdown and overexpression of KDM4B in BMSCs. Chromatin immunoprecipitation (ChIP)-PCR analysis was performed to determine how H3K9me3 and KDM4B affect the regulatory regions of LOX and elastin in BMSCs subjected to 10% CMS combined with KDM4B knockdown. Finally, we verified the expression of KDM4B under CMS in vivo by subcutaneously transplanting KDM4B-overexpressing BMSCs into mice via immunofluorescence assays. The results showed that activating KDM4B by mechanical stretching increased the protein and gene expression levels of LOX and elastin and induced more LOX-positive cells in the BMSCs. These data suggest that CMS contributes to the differentiation of mesenchymal stem cells (MSCs) into fibroblasts, which indicates its potential use as a cell-based therapy for PFD.

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“…As far as their biological applications, HELPs have been widely investigated, in particular, for tissue engineering and regenerative medicine purposes, demonstrating for instance excellent biocompatibility with muscle [7] and stem [8] cells. Recombinant human peptides similar to ours were also proposed by other groups: recombinant versions of human tropoelastin were developed, used in the form of hydrogels and porous membranes and electrospun in elastomeric scaffolds, again for applications in tissue engineering and regenerative medicine [9,10].…”

Section: Human Elastin-like Polypeptidesmentioning

confidence: 99%

The potential of recombinant human elastin-like polypeptides for drug delivery

Ciofani¹,

Genchi²,

Mattoli³

et al. 2014

Expert Opinion on Drug Delivery

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Mimicking the structure of natural proteins by recombinant biopolymers is a useful approach for the development of novel bioactive biomaterials with desired properties, that help elucidate molecular interactions in biological systems and elaborate strategies for tissue engineering and drug delivery purposes. Structurally based on elastin repeated motifs, recombinant human elastin-like polypeptides (HELPs) represent excellent examples of bio-inspired polymers proposed for tissue engineering, and recently exploited also for drug delivery applications. This Editorial reports on the latest advances in the research on HELP biopolymers for drug delivery and targeting applications. The main findings will be summarized with emphasis on the ‘smart’ properties of HELPs, which render this class of biopolymers particularly interesting in the whole biomedicine field. Considerations about further improvements of the current HELP-based systems will be provided, and a demonstration of the huge potential of HELPs in becoming leading material for drug delivery will be attempted

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Human Elastin‐Based Recombinant Biopolymers Improve Mesenchymal Stem Cell Differentiation

Cited by 25 publications

References 50 publications

Elastin-based biomaterials and mesenchymal stem cells

Elastin-based biomaterials and mesenchymal stem cells

Mechanical stretching promotes the differentiation of BMSC into pelvic floor ligament fibroblasts

The potential of recombinant human elastin-like polypeptides for drug delivery

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