Human diploid MRC-5 cells exhibit several critical properties of human umbilical cord-derived mesenchymal stem cells

Zhang, Kehua; Na, Tao; Wang, Lin; Gao, Qiang; Yin, Weidong; Wang, Junzhi; Yuan, Bao‐Zhu

doi:10.1016/j.vaccine.2014.07.071

Cited by 25 publications

(31 citation statements)

References 26 publications

(30 reference statements)

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“…MRC-5, Vero and RK-13 cells were obtained from the American Type Culture Collection (Rockville, MD, USA), all hUC-MSCs were isolated from Wharton’s jelly of human umbilical cord and fully characterized 17 . MRC-5 was cultured in MEM supplemented with 10% FBS (Gibco, Grand Island, NY, USA), Vero and RK-13 were cultured in MEM supplemented with 10% NBS (Sijiqing, Zhejiang, China), hUC-MSCs were cultured in α-MEM supplemented with 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

“…Interestingly, our recent studies demonstrated that many HDCs established from fetal lungs, such as MRC-5, exhibited several critical properties of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs), including cell morphology, growth activity, expression of cell surface markers, abilities to differentiate into multiple cell lineages and immunomodulatory activities 17 . In the meantime, it was found that the (Cell Collection and Research Center-1) cells, an hUC-MSC cell line reported in the previous studies, sustained primitive characteristics during extensive expansion and exhibited a similar sensitivity to the infection of EV71 and Rubella viruses as MRC-5, thus suggesting that hUC-MSCs may meet the same requirements as the traditional HDCs for the production of human vaccines 17 .…”

Section: Introductionmentioning

confidence: 99%

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The hUC-MSCs cell line CCRC-1 represents a novel, safe and high-yielding HDCs for the production of human viral vaccines

Chen

Zhang

et al. 2017

Sci Rep

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MRC-5 represents the most frequent human diploid cells (HDCs)-type cell substrate in the production of human viral vaccines. However, early-passage MRC-5 is diminishing and, due to both technical and ethical issues, it is extremely difficult to derive novel HDCs from fetal lung tissues, which are the common sources of HDCs. Our previous studies suggested that human umbilical cord may represent an alternative but convenient source of new HDCs. Here, we established a three-tiered cell banking system of a hUC-MSC line, designated previously as Cell Collection and Research Center-1 (CCRC-1). The full characterization indicated that the banked CCRC-1 cells were free from adventitious agents and remained non-tumorigenic. The CCRC-1 cells sustained its rapid proliferation even at passage 30 and were susceptible to the infection of a wide spectrum of viruses. Interestingly, the CCRC-1 cells showed much higher production of EV71 or Rubella viruses than MRC-5 and Vero cells when growing in serum-free medium. More importantly, the EV71 vaccine produced from CCRC-1 cells induced immunogenicity while eliciting no detectable toxicities in the tested mice. Collectively, these studies further supported that CCRC-1, and likely other hUC-MSCs as well, may serve as novel, safe and high-yielding HDCs for the production of human viral vaccines.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The hUC-MSCs cell line CCRC-1 represents a novel, safe and high-yielding HDCs for the production of human viral vaccines

Chen

Zhang

et al. 2017

Sci Rep

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“…BD Stemflow hMSC Analysis Kit was employed to detect expression of cell surface markers using FACS Calibur flow cytometer (BD Biosciences) following the procedures reported in a previous study [26]. The data was collected and analyzed by FCS Express V3 software.…”

Section: The Flow Cytometry Assay For Detecting Cell Surface Markersmentioning

confidence: 99%

“…The abilities of hMSC1 or BEAS-2B to differentiate into adipocytes and osteocytes were tested by using STEMPRO Differentiation Kit following the procedures described in a previous report [26]. Briefly, the cells growing in approximately 80% confluence were incubated at 37˚C, 5% CO 2 in each well of a 24-well cell culture plate with each differentiation induction medium for 21 days, then fixed with 4% formaldehyde for 30 min and stained with 0.3% Oil Red O solution for 50 min for testing adipogenesis or with 2% Alizarin Red S solution for 5 min for testing osteogenesis.…”

Section: Osteogenic and Adipogenic Differentiationmentioning

confidence: 99%

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Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells

Han

Wang

et al. 2020

PLoS ONE

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BEAS-2B was originally established as an immortalized but non-tumorigenic epithelial cell line from human bronchial epithelium. Because of general recognition for its bronchial epithelial origin, the BEAS-2B cell line has been widely used as an in vitro cell model in a large variety of studies associated with respiratory diseases including lung carcinogenesis. However, very few studies have discussed non-epithelial features of BEAS-2B cells, especially the features associated with mesenchymal stem cells (MSCs), which represent a group of fibroblast-like cells with limited self-renewal and differentiation potential to various cell lineages. In this study, we compared BEAS-2B with a human umbilical cord-derived MSCs (hMSCs) cell line, hMSC1, which served as a representative of hMSCs in terms of expressing common features of hMSCs. It was observed that both BEAS-2B and hMSC1 shared the same expression profile of surface markers of hMSCs and exhibited similar osteogenic and adipogenic differentiation potential. In addition, like hMSC1, the BEAS-2B cell line exhibited suppressive activities on proliferation of mitogen-activated total T lymphocytes as well as Th1 lymphocytes, and IFNγ-induced expression of IDO1, all thus demonstrating that BEAS-2B cells exhibited an almost identical characteristic profile with hMSCs, even though, there was a clear difference between BEAS-2B and hMSCs in the effects on type 2 macrophage polarization. Most importantly, the hMSCs features of BEAS-2B were unlikely a consequence of epithelial-mesenchymal transition. Therefore, this study provided a set of evidence to provoke reconsideration of epithelial origin of BEAS-2B.

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Synthesis, Molecular Docking, and Anticancer Screening of Ester‐based Thiazole Derivatives

Musa,

Bello,

Agwamba

2024

Chemistry & Biodiversity

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This study investigates the potential of five compounds as novel anticancer agents. We examined their efficacy, mechanisms of action, and impact on various cancer cell lines, through a comprehensive set of experiments. Notably, compound 3e demonstrated superior activity compared to the positive control cisplatin, with a GI50 value of 6.3±0.7 μM against the breast cancer cell line (MCF‐7). Compound 3b also displayed remarkable growth inhibition, yielding GI50 values of 8.7±0.2 μM (MCF‐7) and 8.9±0.5 μM against the colon cancer cell line (HCT‐116). Cell count experiments further confirmed the potent inhibitory effects of compounds 3e, 3b, and 3c on MCF‐7 and HCT‐116 cell growth. Compound 3e demonstrated a reduction of 55–60 % at GI50 and complete inhibition (100 %) at 2x GI50. Compound 3b exhibited 50–55 % reduction (GI50) and 90–95 % inhibition (2x GI50) in HCT‐116 cells. Compound 3c displayed 75–80 % inhibition (2x GI50) and 35–40 % inhibition (GI50) in HCT‐116 cells. In‐depth mechanistic investigations unveiled valuable insights into the mode of action of compound 3e. The cell‐cycle assay demonstrated G2/M phase arrest, DNA damage, and caspase‐mediated apoptosis in both MCF‐7 and HCT‐116 cells. Caspase activation indicated a significant increase in apoptosis following exposure to compound 3e. Furthermore, compound 3e induced reactive oxygen species (ROS) production, influencing HCT‐116 and MCF‐7 cells differently. Elevated ROS production in HCT‐116 cells and distinct effects in MCF‐7 cells contribute to a deeper understanding of the cytotoxic mechanisms of compound 3e. Overall, these findings highlight the potential of the investigated compounds, particularly compound 3e, as effective inducers of apoptosis in cancer cells. Mechanistic insights into cell cycle arrest, caspase‐mediated apoptosis, and ROS modulation provide a comprehensive understanding of their cytotoxic effects. This study offers significant contribution to the development of promising anticancer agents and their therapeutic applications.

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Human diploid MRC-5 cells exhibit several critical properties of human umbilical cord-derived mesenchymal stem cells

Cited by 25 publications

References 26 publications

The hUC-MSCs cell line CCRC-1 represents a novel, safe and high-yielding HDCs for the production of human viral vaccines

The hUC-MSCs cell line CCRC-1 represents a novel, safe and high-yielding HDCs for the production of human viral vaccines

Human lung epithelial BEAS-2B cells exhibit characteristics of mesenchymal stem cells

Synthesis, Molecular Docking, and Anticancer Screening of Ester‐based Thiazole Derivatives

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