Human dehydrogenase/reductase (SDR family) member 11 is a novel type of 17β-hydroxysteroid dehydrogenase

Endo, Shunsuke; Miyagi, Namiki; Matsunaga, Toshiyuki; Hara, Akira; Ikari, Akira

doi:10.1016/j.bbrc.2016.01.190

Cited by 25 publications

(26 citation statements)

References 30 publications

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“…The dehydrogenase/reductase family is short-chain superfamily, encoding various enzymes to participate in the metabolism of steroid hormones [35]. DHRS11 is a novel type of 17 β-hydroxysteroid dehydrogenase to tart part in the synthesis of estrogen and androgen in human [36]. In chicken, DHRS12 has been identified for its plausible function in yolk and follicle developments [37].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Yang

Zhong

et al. 2020

BMC Genomics

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Background: Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the regulatory mechanism of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells isolated from orange-spotted grouper was performed to explore the molecular mechanism underlying spermatogenesis. Results: In this study, the orange-spotted grouper was induced to change sex from female to male by 17alphamethyltestosterone (MT) implantation. During the spermatogenesis, male germ cells (spermatogonia, spermatocytes, spermatids, and spermatozoa) were isolated by laser capture microdissection. Transcriptomic analysis for the isolated cells was performed. A total of 244,984,338 clean reads were generated from four cDNA libraries. Real-time PCR results of 13 genes related to sex differentiation and hormone metabolism indicated that transcriptome data are reliable. RNA-seq data showed that the female-related genes and genes involved in hormone metabolism were highly expressed in spermatogonia and spermatozoa, suggesting that these genes participate in the spermatogenesis. Interestingly, the expression of zbtb family genes showed significantly changes in the RNA-seq data, and their expression patterns were further examined during spermatogenesis. The analysis of cellular localization of Eczbtb40 and the co-localization of Eczbtb40 and Eccyp17a1 in different gonadal stages suggested that Eczbtb40 might interact with Eccyp17a1 during spermatogenesis. Conclusions:Our study, for the first time, investigated the transcriptome of the male germ cells from orangespotted grouper, and identified functional genes, GO terms, and KEGG pathways involved in spermatogenesis. Furthermore, Eczbtb40 was first characterized and its role during spermatogenesis was predicted. These data will contribute to future studies on the molecular mechanism of spermatogenesis in teleosts.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Yang

Zhong

et al. 2020

BMC Genomics

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“…Although several bioreductions of 1‐indanone (Endo et al, ; Fill et al, ) or biooxidations of 1‐indanol (Martin et al, ; Pennacchio et al, ; Pennacchio, Giordano, Pucci, Rossi, & Raia, ; Zhang, Tang, Wang, & Li, ) are mentioned in the literature, none of the involved enzymes could be used in this study, because these enzymes were shown to be either highly enantioselective or NAD + ‐dependent. Ideally, a NADP + ‐linked dehydrogenase with low (if any) enantioselectivity is needed to ensure cofactor recycling and complete transformation of the racemic indanol.…”

Section: Resultsmentioning

confidence: 99%

“…Although several bioreductions of 1-indanone (Endo et al, 2016;Fill et al, 2012) enzymes were able to significantly oxidize cyclohexanol and 18 also 1indanol (Table S4). From these active enzymes, we selected an uncharacterized protein from Singulisphaera acidiphilia (Uniprot ID:…”

Section: Selection Of Enzymes Of the Neutral Redox Cascadementioning

confidence: 99%

Tuning of the enzyme ratio in a neutral redox convergent cascade: A key approach for an efficient one‐pot/two‐step biocatalytic whole‐cell system

Ménil

Petit

Courvoisier-Dezord

et al. 2019

Biotech & Bioengineering

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The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer–Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε‐caprolactone and 3,4‐dihydrocoumarin. This goal was achieved by adjusting the copy number per cell of Escherichia coli plasmids. We started from the observation that this number generally correlates with the amount of produced enzyme and demonstrated that an in vivo multi‐enzymatic system can be improved by the judicious choice of plasmid, the lower activity of the enzyme that drives the limiting step being counter‐balanced by a higher concentration. Using a preconception‐free approach to the choice of the plasmid type, we observed positive and negative synergetic effects, sometimes unexpected and depending on the enzyme and plasmid combinations. Experimental optimization of the culture conditions allowed us to obtain the complete conversion of cyclohexanol (16 mM) and 1‐indanol (7.5 mM) at a 0.5‐L scale. The yield for the conversion of cyclohexanol was 80% (0.7 g ε‐caprolactone, for the productivity of 244 mg·L −1·h −1) and that for 1‐indanol 60% (0.3 g 3,4‐dihydrocoumarin, for the productivity of 140 mg·L −1·h −1).

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Section: Discussionmentioning

confidence: 99%

“…Another higher expressed gene in G2 foundresses that also may be involved in some type of immune response is dehydrogenase/reductase SDR family member 11 (DHRS11 ), a gene encoding an NAD(P)(H)-dependent oxidoreductase that belongs to the large superfamily of shortchain dehydrogenase/reductases (Persson et al 2009;Endo et al 2016). Genes from this superfamily are known to be involved in the metabolism of lipids, carbohydrates, vitamins, drugs, and xenobiotics (Endo et al 2016). Specifically in honeybees, DHRS11 has been found to play a role in host resistance against the varroa mite (Parker et al 2012), and in larval caste differentiation, it appears as one of the genes possibly necessary for worker ovary differentiation (Guidugli et al 2004;Lago et al 2016).…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq reveals that mitochondrial genes and long non-coding RNAs may play important roles in the bivoltine generations of the non-social Neotropical bee Tetrapedia diversipes

2017

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-In animals, voltinism is a result of evolutionary adaptations to environmental conditions. These evolutionary adaptations may profoundly affect the population structure and social organization level. To study the bivoltinism of the solitary bee Tetrapedia diversipes , we performed comparative transcriptomics analyses of foundresses and larvae from the two reproductive generations (G1 and G2) produced per year by this bee. Most of the differentially expressed genes (DEGs) were found between foundresses: 52 DEGs between adults, but only one between the larvae. Among the DEGs in foundresses, 46 were higher expressed in G1 and most of them (38) have no functional annotation defined in the database. Interestingly, mitochondrial genes and long non-coding RNAs were the only type of identified transcripts in the set of upregulated genes. These results highlight the importance of developing studies on non-model species and suggest that maternal genes may be of importance for determining larval diapause in T. diversipes .transcriptome / bivoltinism / diapause / solitary bee / RNA-Seq

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Human dehydrogenase/reductase (SDR family) member 11 is a novel type of 17β-hydroxysteroid dehydrogenase

Cited by 25 publications

References 30 publications

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Tuning of the enzyme ratio in a neutral redox convergent cascade: A key approach for an efficient one‐pot/two‐step biocatalytic whole‐cell system

RNA-Seq reveals that mitochondrial genes and long non-coding RNAs may play important roles in the bivoltine generations of the non-social Neotropical bee Tetrapedia diversipes

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