Human defined antigenic region on the nucleoprotein of Crimean-Congo hemorrhagic fever virus identified using truncated proteins and a bioinformatics approach

Burt, Felicity J.; Samudzi, Rudo; Randall, Charles C.; Pieters, Danelle; Vermeulen, Jan‐G; Knox, Caroline

doi:10.1016/j.jviromet.2013.07.055

Cited by 18 publications

(29 citation statements)

References 16 publications

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“…Thus, the identification and mapping of minimal BCEs on NP represent significant steps in the development of novel diagnostic tools and multi-epitope peptide vaccines. In a previous study, a bacterially expressed recombinant NP antigen was used to detect IgG antibodies against CCHFV; the instability however, of the protein in soluble expression as well as serological diagnosis restricted the application of this protein [47], [48]. The use of non-complete NP or multi-epitope peptides for CCHF diagnosis has attracted increasing attention, along with CCHF studies in general.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, Saijo et al reported that in Western blotting analysis, high titer sera of CCHF patients reacted only with the highly conserved NP fragment which contained the amino acid residues 201 to 306 (NP 201−306 ) [21]. Similarly, Burt et al found that NP 123−396 of CCHFV includes a highly antigenic region with application toward the development of antibody detection assays [48]. Previously, our group showed that NP 237−305 is an immunogenic region of CCHFV-NP using a polyclonal antibody and two monoclonal antibodies against CCHFV with Western blot analysis [22].…”

Section: Discussionmentioning

confidence: 99%

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Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV)

et al. 2014

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Crimean-Congo hemorrhagic fever (CCHF), a severe viral disease known to have occurred in over 30 countries and distinct regions, is caused by the tick-borne CCHF virus (CCHFV). Nucleocapsid protein (NP), which is encoded by the S gene, is the primary antigen detectable in infected cells. The goal of the present study was to map the minimal motifs of B-cell epitopes (BCEs) on NP. Five precise BCEs (E1, 247FDEAKK252; E2a, 254VEAL257; E2b, 258NGYLNKH264; E3, 267EVDKA271; and E4, 274DSMITN279) identified through the use of rabbit antiserum, and one BCE (E5, 258NGYL261) recognized using a mouse monoclonal antibody, were confirmed to be within the central region of NP and were partially represented among the predicted epitopes. Notably, the five BCEs identified using the rabbit sera were able to react with positive serum mixtures from five sheep which had been infected naturally with CCHFV. The multiple sequence alignment (MSA) revealed high conservation of the identified BCEs among ten CCHFV strains from different areas. Interestingly, the identified BCEs with only one residue variation can apparently be recognized by the positive sera of sheep naturally infected with CCHFV. Computer-generated three-dimensional structural models indicated that all the antigenic motifs are located on the surface of the NP stalk domain. This report represents the first identification and mapping of the minimal BCEs of CCHFV-NP along with an analysis of their primary and structural properties. Our identification of the minimal linear BCEs of CCHFV-NP may provide fundamental data for developing rapid diagnostic reagents and illuminating the pathogenic mechanism of CCHFV.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV)

et al. 2014

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“…Typically, transient IgM and IgG antibody responses develop within days following primary CCHFV infection and can persist long-term (Shepherd et al, 1989;Burt et al, 2013), but where lack thereof usually results in fatality (Shepherd et al, 1989). IgM and IgG antibodies have however not been correlated with clearance, viral load, or outcomes (Duh et al, 2007), implying that innate and T cell immunity must be critical for viral clearance.…”

Section: Orthonairovirus Classification Epidemiology Immunology Anmentioning

confidence: 99%

T Lymphocytes as Measurable Targets of Protection and Vaccination Against Viral Disorders

Monette

Mouland

2019

International Review of Cell and Molecular Biology

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Continuous epidemiological surveillance of existing and emerging viruses and their associated disorders is gaining importance in light of their abilities to cause unpredictable outbreaks as a result of increased travel and vaccination choices by steadily growing and aging populations. Close surveillance of outbreaks and herd immunity are also at the forefront, even in industrialized countries, where previously eradicated viruses are now at risk of re-emergence due to instances of strain recombination, contractions in viral vector geographies, and from their potential use as agents of bioterrorism. There is a great need for the rational design of current and future vaccines targeting viruses, with a strong focus on vaccine targeting of adaptive immune effector memory T cells as the gold standard of immunity conferring long-lived protection against a wide variety of pathogens and malignancies. Here, we review viruses that have historically caused large outbreaks and severe lethal disorders, including respiratory, gastric, skin, hepatic, neurologic, and hemorrhagic fevers. To observe trends in vaccinology against these viral disorders, we describe viral genetic, replication, transmission, and tropism, host-immune evasion strategies, and the epidemiology and health risks of their associated syndromes. We focus on immunity generated against both natural infection and vaccination, where a steady shift in conferred vaccination immunogenicity is observed from quantifying activated and proliferating, long-lived effector memory T cell subsets, as the prominent biomarkers of long-term immunity against viruses and their associated disorders causing high morbidity and mortality rates.

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“…The CCHFV nucleocapsid protein (NP) is known to be the most immunogenic and predominant antigen expressed early after infection; therefore, it is frequently used for developing diagnostic assays [30,41]. Because the CCHFV is a BSL-4 pathogen, the preparation of the authentic CCHFV antigens from infected cell cultures or infected mouse brain cells for the development of antibody detection systems must be performed in a BSL-4 laboratory.…”

Section: Antibody Detectionmentioning

confidence: 99%

Diagnosis of Crimean-Congo hemorrhagic fever

Tezer

Polat²

2015

Expert Review of Anti-infective Therapy

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Crimean-Congo hemorrhagic fever (CCHF) virus is the most extensive tick-borne virus, it causes a severe infection, which occurs widely in Africa, Eastern Europe and Asia. In recent years, the dramatic increase in the global distribution of CCHF, with the high mortality rates, highlights the importance of improving diagnostic capacity. Clinical and epidemiological data play a crucial role for early recognition of CCHF. However, CCHF is clinically difficult to diagnose and to distinguish, a rapid and reliable laboratory confirmation is necessary. Confirmation of infection in the acute phase of the disease can be made by detection of viral nucleic acid using reverse transcription-PCR, by demonstration of viral antigen or by virus isolation. In the convalescent phase of the disease, the diagnosis is confirmed by demonstration of an antibody response. The consideration of viral replication kinetics and antiviral humoral immune responses facilitates the selection of appropriate laboratory tests and accurate interpretation of laboratory findings.

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Human defined antigenic region on the nucleoprotein of Crimean-Congo hemorrhagic fever virus identified using truncated proteins and a bioinformatics approach

Cited by 18 publications

References 16 publications

Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV)

Fine Epitope Mapping of the Central Immunodominant Region of Nucleoprotein from Crimean-Congo Hemorrhagic Fever Virus (CCHFV)

T Lymphocytes as Measurable Targets of Protection and Vaccination Against Viral Disorders

Diagnosis of Crimean-Congo hemorrhagic fever

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