Human decidual stromal cells secrete soluble pro-apoptotic factors during decidualization in a cAMP-dependent manner

Leno-Durán, Ester; Ruiz-Magaña, María José; Muñoz-Fernández, Raquel; Requena, Francisco; Olivares, Enrique G.; Ruiz‐Ruiz, Carmen

doi:10.1093/humrep/deu202

Cited by 27 publications

(16 citation statements)

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“…In this connection, we previously confirmed that the antigen phenotype of preDSCs is compatible with MSCs [5,19,25]. Functions such as cell contractility, resistance to apoptosis, and inhibition of lymphocyte apoptosis are also shared by MSCs and preDSCs [18,[26][27][28][29][30]. Further evidence of a close relationship between preDSCs and MSCs was also reported by Dimitrov et al [31].…”

Section: (Continued From Previous Page)supporting

confidence: 81%

Human predecidual stromal cells are mesenchymal stromal/stem cells and have a therapeutic effect in an immune-based mouse model of recurrent spontaneous abortion

et al. 2019

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Background: Human decidual stromal cells (DSCs) are involved in the maintenance and development of pregnancy, in which they play a key role in the induction of immunological maternal-fetal tolerance. Precursors of DSCs (preDSCs) are located around the vessels, and based on their antigen phenotype, previous studies suggested a relationship between preDSCs and mesenchymal stromal/stem cells (MSCs). This work aimed to further elucidate the MSC characteristics of preDSCs. Methods:We established 15 human preDSC lines and 3 preDSC clones. Physiological differentiation (decidualization) of these cell lines and clones was carried out by in vitro culture with progesterone (P4) and cAMP. Decidualization was confirmed by the change in cellular morphology and prolactin (PRL) secretion, which was determined by enzyme immunoassay of the culture supernatants. We also studied MSC characteristics: (1) In mesenchymal differentiation, under appropriate culture conditions, these preDSC lines and clones differentiated into adipocytes, osteoblasts, and chondrocytes, and differentiation was confirmed by cytochemical assays and RT-PCR. (2) The expression of stem cell markers was determined by RT-PCR. (3) Cloning efficiency was evaluated by limited dilution. (4) Immunoregulatory activity in vivo was estimated in DBA/2-mated CBA/J female mice, a murine model of immune-based recurrent abortion. (5) Survival of preDSC in immunocompetent mice was analyzed by RT-PCR and flow cytometry.Results: Under the effect of P4 and cAMP, the preDSC lines and clones decidualized in vitro: the cells became rounder and secreted PRL, a marker of physiological decidualization. PreDSC lines and clones also exhibited MSC characteristics. They differentiated into adipocytes, osteoblasts, and chondrocytes, and preDSC lines expressed stem cell markers OCT-4, NANOG, and ABCG2; exhibited a cloning efficiency of 4 to 15%; significantly reduced the embryo resorption rate (P < 0.001) in the mouse model of abortion; and survived for prolonged periods in immunocompetent mice. The fact that 3 preDSC clones underwent both decidualization and mesenchymal differentiation shows that the same type of cell exhibited both DSC and MSC characteristics.(Continued on next page)

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Section: (Continued From Previous Page)supporting

confidence: 81%

Human predecidual stromal cells are mesenchymal stromal/stem cells and have a therapeutic effect in an immune-based mouse model of recurrent spontaneous abortion

et al. 2019

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“…The stromal cells isolated from the decidua parietalis was confirmed to be of maternal origin31. To induce decidualization, stromal cells (2 × 10 5 cells/ml) were cultured in 24-well plates in complete DMEM in the presence of 300 nM progesterone (P4; Sigma-Aldrich) and 500 μM 8-bromo-cAMP (Sigma-Aldrich) for 7 days32. Half of the culture medium was changed on day 4, and P4 and cAMP were readded.…”

Section: Methodsmentioning

confidence: 99%

IFN type I and II induce BAFF secretion from human decidual stromal cells

Lundell

Nordström

Andersson

et al. 2017

Sci Rep

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B cell activating factor (BAFF) is a critical cytokine for maturation of immature B cells. In murine lymph nodes, BAFF is mainly produced by podoplanin-expressing stromal cells. We have previously shown that circulating BAFF levels are maximal at birth, and that farmers’ children exhibit higher BAFF levels in cord blood than non-farmers’ children. Here, we sought to investigate whether maternal-derived decidual stromal cells from placenta secrete BAFF and examine what factors could stimulate this production. We found that podoplanin is expressed in decidua basalis and in the underlying villous tissue as well as on isolated maternal-derived decidual stromal cells. Decidual stromal cells produced BAFF when stimulated with IFN-γ and IFN-α, and NK cells and NK-T-like cells competent of IFN-γ production were isolated from the decidua. Finally, B cells at different maturational stages are present in decidua and all expressed BAFF-R, while stromal cells did not. These findings suggest that decidual stromal cells are a cellular source of BAFF for B cells present in decidua during pregnancy.

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“…Resveratrol has anti‐inflammatory actions that may suppress embryo implantation directly. The decidualization of endometrial stromal cells does not entail only cellular differentiation; the alteration requires a combination of differentiation and apoptosis/senescence . In fact, decidual cells secrete proapoptotic factors during the decidualization of HESCs .…”

Section: Impact On Endometrium For Pregnancymentioning

confidence: 99%

“…The decidualization of endometrial stromal cells does not entail only cellular differentiation; the alteration requires a combination of differentiation and apoptosis/senescence . In fact, decidual cells secrete proapoptotic factors during the decidualization of HESCs . In in vitro primary cultures, the decidualization of HESCs induces senescence‐associated β‐galactosidase (SAβG) activity and increases expressions of major senescent markers, including p16 and p53 .…”

Section: Impact On Endometrium For Pregnancymentioning

confidence: 99%

“…[56][57][58] In fact, decidual cells secrete proapoptotic factors during the decidualization of HESCs. 58 In in vitro primary cultures, the decidualization of HESCs induces senescence-associated β-galactosidase (SAβG) activity and increases expressions of major senescent markers, including p16 and p53. 56,57 Decidual cells secrete inflammatory mediators associated with endometrial receptivity, through acute cellular senescence.…”

Section: Effects On Human Endometrium In Vitromentioning

confidence: 99%

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Preconception resveratrol intake against infertility: Friend or foe?

Ochiai

Kuroda

2019

Reprod Medicine & Biology

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Background Resveratrol is an antiaging, antioxidant, anti‐inflammatory, and insulin‐sensitizing natural polyphenolic compound. Growing evidence indicates that resveratrol has potential therapeutic effects in infertile women with diminished ovarian function, polycystic ovary syndrome (PCOS), or endometriosis. However, only one clinical trial in women undergoing in vitro fertilization (IVF) cycles using resveratrol has ever been reported. This review focuses on the potential therapeutic effects of resveratrol on pregnancy and on its advantages and disadvantages in pregnancy outcomes during infertility treatment. Methods We performed a literature review to describe the known impacts of resveratrol on the ovary and endometrium. Results Resveratrol upregulates sirtuin (SIRT)1 expression in ovaries, which is associated with protection against oxidative stress. It leads to the activation of telomerase activity and mitochondrial function, improving ovarian function. In the endometrium, resveratrol downregulates the CRABP2‐RAR pathway leading to suppressing decidual and senescent changes of endometrial cells, which is essential for embryo implantation and placentation. Moreover, resveratrol may also induce deacetylation of important decidual‐related genes. Conclusions Resveratrol has potential therapeutic effects for improving ovarian function; however, it also has anti‐deciduogenic actions in uterine endometrium. In addition, its teratogenicity has not yet been ruled out; thus, resveratrol should be avoided during the luteal phase and pregnancy.

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Human decidual stromal cells secrete soluble pro-apoptotic factors during decidualization in a cAMP-dependent manner

Cited by 27 publications

References 50 publications

Human predecidual stromal cells are mesenchymal stromal/stem cells and have a therapeutic effect in an immune-based mouse model of recurrent spontaneous abortion

Human predecidual stromal cells are mesenchymal stromal/stem cells and have a therapeutic effect in an immune-based mouse model of recurrent spontaneous abortion

IFN type I and II induce BAFF secretion from human decidual stromal cells

Preconception resveratrol intake against infertility: Friend or foe?

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