Human decellularized adipose tissue scaffold as a model for breast cancer cell growth and drug treatments

Dunne, Lina W.; Huang, Zhao; Meng, Wenxiang; Fan, Xuejun; Zhang, Ningyan; Zhang, Qixu; An, Zhiqiang

doi:10.1016/j.biomaterials.2014.03.003

Cited by 134 publications

(129 citation statements)

References 52 publications

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“…These matrices were used for investigating the behavior of breast tumor-derived cells (proliferation, morphology, migration) and their drug sensitivity, that were shown to be quite similar to what can be obtained with the in vivo xenograft models [126]. In particular ECM, prepared by using different explants obtained from human intestinal mucosae (ranging from healthy colon to colorectal carcinoma), can also be used to investigate tumor cell's behavior in response to a more or less permissive microenvironment [127]. The main limit of this ECM is that they are natively complex, their exact composition is not fully known, and this may render difficult the interpretation of cell's behavior in response to a particular stimulus.…”

Section: Mimicking the Ecmmentioning

confidence: 96%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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Section: Mimicking the Ecmmentioning

confidence: 96%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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“…Thus, 3D culture may be a powerful tool in tumor and relevant drug research. Marked advances have been made in the basic development of 3D tumor models so far, and prominent studies using 3D tumor cell models to simulate the tumor microenvironment or assess drug delivery in the past 5 years are summarized in Table I (6,8,(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35). Considering the advantages of 3D tumor models, the present review provides an overview of the methods and techniques successfully devised for practicing 3D tumor cell culture.…”

Section: Current Preclinical Tumor Modelsmentioning

confidence: 99%

Three‑dimensional cell culture: A powerful tool in tumor research and drug discovery (Review)

Lv¹,

Hu²,

Lü³

et al. 2017

Oncol Lett

268

296

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Abstract. In previous years, three-dimensional (3D) cell culture technology has become a focus of research in tumor cell biology, using a variety of methods and materials to mimic the in vivo microenvironment of cultured tumor cells ex vivo. These 3D tumor cells have demonstrated numerous different characteristics compared with traditional two-dimensional (2D) culture. 3D cell culture provides a useful platform for further identifying the biological characteristics of tumor cells, particularly in the drug sensitivity area of the key points of translational medicine. It promises to be a bridge between traditional 2D culture and animal experiments, and is of great importance for further research in the field of tumor biology. In the present review, previous 3D cell culture applications, focusing on anti-tumor drug susceptibility testing, are summarized.

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“…Interface 14: 20170137 prepared by freeze drying, electrospinning, phase separation and microscale macromolecular self-assembly [70][71][72][73][74]. Tumour cells cultured in scaffolds showed less sensitivity to chemotherapy and yield tumours with more invasive phenotypes [70,[75][76][77]. While the porous scaffolds have the mechanical and chemical characteristic of ECM for threedimensional tumour cell culture, the disadvantages include lack of vasculature structure in fabricated scaffolds that hinder perfusion for long-term culture, as well as poor control on cell placement positions inside the scaffold.…”

Section: Three-dimensional Cell Culture In Three-dimensional Matricesmentioning

confidence: 99%

Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

Tsai

Trubelja

Shen

et al. 2017

J. R. Soc. Interface.

168

133

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Cancer remains one of the leading causes of death, albeit enormous efforts to cure the disease. To overcome the major challenges in cancer therapy, we need to have a better understanding of the tumour microenvironment (TME), as well as a more effective means to screen anti-cancer drug leads; both can be achieved using advanced technologies, including the emerging tumour-on-a-chip technology. Here, we review the recent development of the tumour-on-a-chip technology, which integrates microfluidics, microfabrication, tissue engineering and biomaterials research, and offers new opportunities for building and applying functional three-dimensional in vitro human tumour models for oncology research, immunotherapy studies and drug screening. In particular, tumour-on-a-chip microdevices allow well-controlled microscopic studies of the interaction among tumour cells, immune cells and cells in the TME, of which simple tissue cultures and animal models are not amenable to do. The challenges in developing the next-generation tumour-on-a-chip technology are also discussed.

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Human decellularized adipose tissue scaffold as a model for breast cancer cell growth and drug treatments

Cited by 134 publications

References 52 publications

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Three‑dimensional cell culture: A powerful tool in tumor research and drug discovery (Review)

Tumour-on-a-chip: microfluidic models of tumour morphology, growth and microenvironment

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