2009
DOI: 10.1128/jvi.00695-09
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Human Cytomegalovirus UL131-128 Genes Are Indispensable for Virus Growth in Endothelial Cells and Virus Transfer to Leukocytes

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Cited by 81 publications
(180 citation statements)
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“…This technique (i) greatly facilitates genetic manipulation of viral genes in the context of the viral genome, (ii) enables amplification of the genome in the absence of selective pressure, and (iii) yields clonal viral genomes without the need of plaque purifications (Brune et al, 1999;Messerle et al, 2000). Here, we report generation of BACs derived from TB40/E in order to yield genetically pure and highly endotheliotropic clones of this virus and to test the hypothesis that the phenotype of variants contained within TB40/E is determined by the UL128-UL131A gene region previously linked to loss of EC tropism during extended fibroblast adaptation (Adler et al, 2006;Hahn et al, 2004;Wang & Shenk, 2005b).…”
Section: Introductionmentioning
confidence: 99%
“…This technique (i) greatly facilitates genetic manipulation of viral genes in the context of the viral genome, (ii) enables amplification of the genome in the absence of selective pressure, and (iii) yields clonal viral genomes without the need of plaque purifications (Brune et al, 1999;Messerle et al, 2000). Here, we report generation of BACs derived from TB40/E in order to yield genetically pure and highly endotheliotropic clones of this virus and to test the hypothesis that the phenotype of variants contained within TB40/E is determined by the UL128-UL131A gene region previously linked to loss of EC tropism during extended fibroblast adaptation (Adler et al, 2006;Hahn et al, 2004;Wang & Shenk, 2005b).…”
Section: Introductionmentioning
confidence: 99%
“…Mutations in the UL131A-UL128 gene locus are sufficient to eliminate epithelial/endothelial tropism and occur spontaneously within only a few passages of wild-type (WT) HCMV in fibroblasts (15,16). In addition, Pentamer cell surface overexpression interferes with HCMV entry into epithelial cells, but not into fibroblasts, suggesting the presence of a cell-typespecific Pentamer receptor (17).…”
mentioning
confidence: 99%
“…The IgG antibody responses to the glycoprotein complexes representing the pentamer (gH/gL/ pUL128L), the dimer gH/gL (Hahn et al, 2004;Ryckman et al, 2008) and gB were investigated. In addition, the neutralizing antibody response was determined in parallel by inoculating virus-serum mixtures onto ARPE-19 epithelial cells and HELF cells.…”
Section: Discussionmentioning
confidence: 99%
“…In 2004, in collaboration with the group of U. Koszinowksi (Munchen, Germany), it was documented for the first time that HCMV UL128-131 genes are indispensable for virus growth in endothelial cells and virus transfer to leukocytes (Hahn et al, 2004). Subsequently, it was shown that the UL128, UL130 and UL131 locus (thus referred to as UL128L) gene products formed a pentameric complex with gH/gL (gH/gL/pUL128/pUL130/pUL131), which was required for infection of both endothelial and epithelial cells (Wang & Shenk, 2005;Ryckman et al, 2008;.…”
Section: Introductionmentioning
confidence: 99%