Human cytomegalovirus strain towne glycoprotein B is processed by proteolytic cleavage

Spaete, Richard R.; Thayer, Richard M.; Probert, William S.; Masiarz, Frank R.; Chamberlain, Scott; Rasmussen, Lucy; Merigan, Thomas C.; Pachl, Carol

doi:10.1016/0042-6822(88)90071-2

Cited by 183 publications

(213 citation statements)

References 68 publications

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“…However, mechanisms of HCMV to escape humoral immune response, as shown for human immunodeficiency virus, have not been described (Schreiber et al, 1997). Neutralizing antibodies bind to several conformation-dependent antigenic epitopes of gB and to the linear epitopes within antigenic domains AD1 and AD2 (Britt & Mach, 1996 ;Spaete et al, 1988). The cross-neutralization activity of monoclonal antibodies directed against these epitopes has not been investigated using different clinical HCMV strains.…”

Section: Discussionmentioning

confidence: 99%

“…gB plays an important role in virus infectivity and cell-to-cell spread, and is a major target for antibody-mediated immunity (Navarro et al, 1993 ;Britt et al, 1990 ;Utz et al, 1989). The linear epitopes AD1 (located on gp 55) and AD2 (located at the N terminus of gp116) bind virus-neutralizing antibodies (Britt & Mach, 1996 ;Spaete et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination.

Haberland¹,

Meyer-K√∂nig²,

Hufert³

1999

Journal of General Virology

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Human cytomegalovirus (HCMV) strains can be classified into different glycoprotein B (gB) genotypes. In a previous study, frequent intragenic variation of the gB gene was shown. The aim of this study was to analyse whether gB variation was due to homologous recombination. The gB gene of DNA extracts derived from the peripheral blood leukocytes of 14 immunosuppressed patients was amplified by PCR and cloned. Three variable sites of gB were analysed by restriction fragment analysis and DNA sequencing and compared with published prototypic strains. In three patients doubly infected with two distinct HCMV gB strains, prototypic (60-85 %) and nonprototypic recombinant strains (5-40 %) were detected. To demonstrate that homologous recombination is driving HCMV gB variability, cells were coinfected with plaque-purified prototypic gB strains and recombinant gB genes were selectively amplified by PCR. gB recombinants were detected after 15 days of coculture and cross-over sites were determined by sequencing. These data indicate that homologous recombination contributes to the variability of the gB gene in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination.

Haberland¹,

Meyer-K√∂nig²,

Hufert³

1999

Journal of General Virology

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“…The predicted topology of the gp55 transmembrane region indicates that the N and C-terminal parts of the molecule could be on the same side of the membrane (Spaete et al, 1988). If so, the antigenic region characterized could be exposed on the outer surface of the envelope with no need for antigen processing to elicit IgG reactivity.…”

Section: Discussionmentioning

confidence: 99%

“…The peptide segments synthesized represented aa 451 to 907 of the HCMV strain AD169 gB sequence (Spaete et al, 1988). Amino acid analysis (Institute of Biochemistry, Biomedical Center, University of Uppsala, Sweden) of the pin peptides (10 aa) indicated the total amount of residue to be 12 to 29 gg per pin.…”

Section: Methodsmentioning

confidence: 99%

“…Herpes simplex virus type 1 (HSV-1) gB contains neutralizing epitopes and is thought to have fusogenic properties responsible for virus penetration into cells (Pereira et al, 1989); a similar function for the HCMV homologue has been proposed (Spear, 1985). The HCMV gB gene encodes a 130K precursor protein (gpl30) which is thought to be cleaved by a cellular enzyme, giving rise to a C-terminal 55K glycoprotein, designated gp55 (Spaete et al, 1988). The Mr of the gB complex has been shown to vary (Pereira et al, 1984;Britt, 1984;Britt & Auger, 1986;G6ncz61 et at., 1986;Landini et al, 1987;Mach et al, 1986;Cranage et al, 1986), but its abundance in the virion and immunological importance make it likely that the same glycoprotein was detected in all investigations.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Major Antigenic Region on gp55 of Human Cytomegalovirus

Silvestri

Sundqvist²,

Rudén³

et al. 1991

Journal of General Virology

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A major antigenic region localized to the C-terminal part of the 55K glycoprotein of human cytomegalovirus (HCMV) was mapped using synthetic peptides. Analysis of the region with six sera from healthy anti-HCMV seropositive blood donors showed that the length of the reactive sequence varied between four and eight amino acids (aa). The shortest sequence recognized was VTSG (aa 793 to 801 of the 130K precursor protein), but most sera required the three or four residues C-terminal to this to react, giving a major site ofVTSGSTKD (aa 798 to 805). Using peptides immobilized on polyethylene pins, the importance of both interior (between aa essential for the binding of an antibody) and extension spacer residues was evident. Free peptides containing the reactive sequence were prepared for use in a conventional ELISA and optimal pH conditions for coating were determined. The best results were achieved at pH 2 to 3, which is in agreement with the advantageous net charge of these peptides at this low pH. Anti-HCMV positive sera showed a sensitivity of approximately 50% for both peptides on polyethylene pins and peptides coated onto plates.

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Recombinant cytomegalovirus glycoprotein gB (UL55) induces an autoantibody response to the U1-70 kDa small nuclear ribonucleoprotein

1999

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Human cytomegalovirus strain towne glycoprotein B is processed by proteolytic cleavage

Cited by 183 publications

References 68 publications

Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination.

Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination.

Characterization of a Major Antigenic Region on gp55 of Human Cytomegalovirus

Recombinant cytomegalovirus glycoprotein gB (UL55) induces an autoantibody response to the U1-70 kDa small nuclear ribonucleoprotein

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