Human Cytomegalovirus Persistent Infection in a Human Central Nervous System Cell Line: Production of a Variant Virus with Different Growth Characteristics

Ogura, Tsutomu; Tanaka, Junji; Kamiya, Shigeru; Sato, Hiroshi; Ogura, Hisashi; Hatano, Miyako

doi:10.1099/0022-1317-67-12-2605

Cited by 41 publications

(37 citation statements)

References 30 publications

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“…There have been three previous reports of the establishment of persistent HCMV infections in brain cells (7,8,23). Ogura and colleagues established a persistent HCMV infection in cells of the permissive 118MGC (glioma) cell line.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Infection and Shedding of Human Cytomegalovirus in T98G Glioblastoma Cells

Luo

Fortunato

2007

J Virol

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Section: Discussionmentioning

confidence: 99%

Long-Term Infection and Shedding of Human Cytomegalovirus in T98G Glioblastoma Cells

Luo

Fortunato

2007

J Virol

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“…U373MG cells expressing IE1 may provide an interesting model for study of the viral gene in glial tumors. The pathogenesis of glial tumors is likely to involve persistent infection allowing expression of only a limited number of viral genes, rather than productive infection, which may lead to cell death directly or indirectly by immune responses (Ogura et al, 1986;Poland et Wolff et al, 1994). U373MG-IE1 cells used in this study may simulate such an in vivo situation.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of GFAP, TSP‐1, and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus

Lee

Jeon

Kim

et al. 2005

Glia

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Human cytomegalovirus (HCMV) is a member of the b-herpesvirus family, which has tropism for glial cells. It was recently reported that HCMV might play important roles in the pathogenesis of malignant glioma. In this study, we investigated the effects of the HCMV IE1 protein on the gene expression profile in the human glioblastoma cell line, U373MG by employing cDNA microarray technology. Using DNA chips containing approximately 1,000 human cDNAs, RNA samples from U373MG cells stably expressing IE1 were compared with those from the control cells lacking IE1 cDNA. Fluorescence intensities of 13 genes were significantly decreased in IE1-expressing cells, while one gene was found to be upregulated. Among these 14 genes, we chose to work further on glial fibrillary acidic protein (GFAP), thrombospondin-1 (TSP-1), and p53, because of their previously known involvement in tumorigenesis. The mRNA levels of all these genes were found to be decreased in IE1-expressing glioblastoma cells by real-time quantitative reverse transcriptionpolymerase chain reaction (RT-PCR) as well as Northern blot analysis. The decreased expression of these genes was also observed at protein levels as measured by immunocytochemistry or fluorescence-activated cell sorting (FACS) analysis. Our data strongly suggested that HCMV IE1 could modulate the expression of cellular genes that might play important roles in the pathogenesis of glial tumors. '

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“…In vitro models of HCMV brain cell infection have been established to attempt to gain a better understanding of HCMV replication in brain cells. In vitro, HCMV is capable of replicating in primary brain cells and several cell lines of nervous tissue origin (Wroblewska et al, 1981;Ogura et al, 1986;Kari & Gehrz 1986;Duclos et al, 1989;Poland et al, 1990). Primary brain cells are capable of producing infectious HCMV; however, these cells are slow to release infectious virus, 60 to 70% of infectious HCMV remaining cell-associated 9 to 11 days post-infection (p.i.).…”

Section: Introductionmentioning

confidence: 99%

Processing of human cytomegalovirus envelope glycoproteins in and egress of cytomegalovirus from human astrocytoma cells

Kari

Radeke²,

Gehrz³

1992

Journal of General Virology

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The synthesis of human cytomegalovirus (HCMV) envelope glycoproteins and the production of infectious HCMV in human astrocytoma and skin fibroblast (SF) cells were analysed. HCMV envelope glycoproteins synthesized in astrocytoma cells had lower Mrs than the same glycoproteins synthesized in SF cells regardless of the strain of HCMV used, showing that the differences observed were due to differences in processing by the host cell and not the strain of HCMV used. HCMV envelope glycoproteins synthesized in astrocytoma cells were found to contain less galactosamine. Moreover, when synthesized in SF cells some HCMV glycoproteins contained a protease-resistant fragment owing to the presence of a cluster of O-linked oligosaccharides on the polypeptide. This fragment was not present when these HCMV glycoproteins were synthesized in astrocytoma cells. These data suggested that HCMV glycoproteins synthesized in astrocytoma cells contain fewer O-linked oligosaccharides. In contrast, other post-translational events such as proteolytic cleavage of the HCMV gB glycoprotein and the formation of disulphide-linked complexes did occur. The virus produced in astrocytoma ceils was capable of infecting SF cells, suggesting that complete O-glycosylation is not needed to produce infectious HCMV. However, astrocytoma cells were slow to release virus into the culture medium, suggesting that a fully functional Golgi network is needed for efficient egress of HCMV from the host cell.

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Human Cytomegalovirus Persistent Infection in a Human Central Nervous System Cell Line: Production of a Variant Virus with Different Growth Characteristics

Cited by 41 publications

References 30 publications

Long-Term Infection and Shedding of Human Cytomegalovirus in T98G Glioblastoma Cells

Long-Term Infection and Shedding of Human Cytomegalovirus in T98G Glioblastoma Cells

Downregulation of GFAP, TSP‐1, and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus

Processing of human cytomegalovirus envelope glycoproteins in and egress of cytomegalovirus from human astrocytoma cells

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