Human Cytomegalovirus Infection and Cardiovascular Disease: Current Perspectives

Lee, Silvia; Affandi, Jacquita S.; Waters, Shelley; Price, Patricia

doi:10.1089/vim.2022.0139

Cited by 10 publications

(4 citation statements)

References 114 publications

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“…HCMV + patients within each defined patient group (CAD/diabetes groups: number of tests = 4). , following by generating a lymphocyte gate [3] and removing of dead cells [4], CD14 + cells [5], and CD1c + cells [6]. The remaining cells were then plotted by CD3 (T cells) vs. CD19 (B cells) [7], and CD3 -CD19cells were plotted by CD56 vs. CD16 to identify NK cells [8].…”

Section: Discussionmentioning

confidence: 99%

“…CAD severity is reported to increase with diabetes, likely through plaque remodeling 2 , but the mechanisms are not fully understood. Several other CAD risk factors are HCMV infection 3 , smoking, obesity, increased LDL and re-duced HDL, and high blood pressure 1 . CAD immune responses are described as increased Th1 responses, associated with increased IFN-γ and chronic low-grade inflammation, macrophage infiltration in the neo-intima, foam cell formation, and decreased smooth muscle cell proliferation 4,5 .…”

Section: Introductionmentioning

confidence: 99%

“…G) Cytek analysis of CAD cohort patients' PBMC (n = 61, without CAD or diabetes status. Upper panels: Cytek gating strategy of PBMC: PBMC were gated to exclude doublets by FSC-A vs. FSC-H [1] and FSC-A vs. FSC-W [2], following by generating a lymphocyte gate[3] and removing of dead cells[4], CD14 + cells[5], and CD1c + cells[6]. The remaining cells were then plotted by CD3 (T cells) vs. CD19 (B cells)[7], and CD3 -CD19cells were plotted by CD56 vs. CD16 to identify NK cells[8].…”

mentioning

confidence: 99%

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CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

Armstrong,

Chen,

Kumar

et al. 2024

Preprint

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Coronary artery disease (CAD) is a leading cause of mortality worldwide with Diabetes and human cytomegalovirus (HCMV) infection as risk factors. CAD's influence on human NK cells is not well characterized. CITE-seq analysis of a CAD cohort of 61 patients revealed distinctly higher NK cell SPON2 expression and lower IFNG expression in severe CAD patients. Interestingly, HCMV+ patients displayed lower SPON2 expression while diabetes status reversed the HCMV effect. Diabetes led to diminished adaptive FcεRIγ -/low NK cell frequencies and was associated with a higher PBMC IL15/TGFB transcript ratio, while TGFB increased in severe CAD. SPON2 expression corresponded to changes in conventional vs. adaptive NK cell frequencies, and the SPON2/IFNG ratio decreased in inflamed plaque tissue with an increased adaptive NK cell gene signature and was increased in severe CAD patients. Our results indicate that the SPON2/IFNG ratio and adaptive NK cell gene signature associated with stenosis severity or inflammation in CAD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

Armstrong,

Chen,

Kumar

et al. 2024

Preprint

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“…Additionally, we aimed to examine the differences in seroprevalence between the two periods, which were a decade apart. Furthermore, we performed a comparative study investigating the presence of HCMV chronic infection and anti-HCMV IgG antibody titres among healthy controls and individuals with HCMV-associated inflammatory-based diseases, including cardiovascular diseases such as aortic stenosis, aortic or mitral pathology and coronary artery disease (CVD) [ 11 , 12 ], serious mental illness (SMI) [ 13 , 14 ], and the novel COVID-19 [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Spanish HCMV Seroprevalence in the 21st Century

Álvarez-Heredia,

Reina-Alfonso,

Domínguez-del-Castillo

et al. 2023

Viruses

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Human cytomegalovirus (HCMV) is linked to age-related diseases like cardiovascular disease, neurodegenerative conditions, and cancer. It can also cause congenital defects and severe illness in immunocompromised individuals. Accurate HCMV seroprevalence assessment is essential for public health planning and identifying at-risk individuals. This is the first HCMV seroprevalence study conducted in the general Spanish adult population in 30 years. We studied HCMV seroprevalence and HCMV IgG antibody titres in healthy adult donors (HDs) and HCMV-related disease patients from 2010 to 2013 and 2020 to 2023, categorized by sex and age. We compared our data with 1993 and 1999 studies in Spain. The current HCMV seroprevalence among HDs in Spain is 73.48%. In women of childbearing age, HCMV seroprevalence has increased 1.4-fold in the last decade. HCMV-seropositive individuals comprise 89.83% of CVD patients, 69% of SMI patients, and 70.37% of COVID-19 patients. No differences in HCMV seroprevalence or HCMV IgG antibody titres were observed between patients and HDs. A significant reduction in Spanish HCMV seroprevalence among HDs was observed in 1993. However, women of childbearing age have shown an upturn in the last decade that may denote a health risk in newborns and a change in HCMV seroprevalence trends.

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Microchip construction for migration assays: investigating the impact of physical confinement on cell morphology and motility during vaccinia virus infection

Wang,

Huangfu,

Wang

et al. 2024

Anal Bioanal Chem

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Human Cytomegalovirus Infection and Cardiovascular Disease: Current Perspectives

Cited by 10 publications

References 114 publications

CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

CITE-seq analysis reveals human cytomegalovirus and diabetes-associated adaptive NK cell alterations in cardiovascular disease

Spanish HCMV Seroprevalence in the 21st Century

Microchip construction for migration assays: investigating the impact of physical confinement on cell morphology and motility during vaccinia virus infection

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