Human cytomegalovirus DNA in cerebrospinal fluid.

Kohyama, Jun; Kajiwara, Michiko; Shimohira, Masayuki; Iwakawa, Yoshihide; Okawa, Hitoshi

doi:10.1136/adc.71.5.414

Cited by 12 publications

(12 citation statements)

References 30 publications

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“…The detection of HCMV in CSF specimens is the best viral marker of infection in the CNS with sensitivity of 98% and specificity of 94%. 13,25 In patient 3A with calcifications on brain CT, DNA HCMV was found in CSF at the age of 12 months. Even in one patient who demonstrated calcifications with pachygyria-polymicrogyria (intrauterine HCMV infection), long-term interval from onset of infection and lumbar puncture pleocytosis and protein concentrations were normal.…”

Section: Discussionmentioning

confidence: 99%

“…CMV may also persist in the CNS for many years in latent form, 4,25 and in rare cases causes Rassmussen encephalitis 11 brain biopsy or investigation of the material from resection of cortical dysplasia could confirm CNS involvement with HCMV. The detection of HCMV in CSF specimens is the best viral marker of infection in the CNS with sensitivity of 98% and specificity of 94%.…”

Section: Discussionmentioning

confidence: 99%

“…4,25 Negative control CSF for DNA HCMV in all infants with neuroinfection is evidence of antiviral treatment efficacy. We observed infants with HCMV neuroinfection and intractable epileptic spasms in accordance with the study by Voudris et al, 24 who also reported intractable infantile spasms after GCV therapy.…”

Section: Discussionmentioning

confidence: 99%

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Infantile spasms and cytomegalovirus infection: antiviral and antiepileptic treatment

Dunin-Wąsowicz

Kasprzyk-Obara

Jurkiewicz

et al. 2007

Develop Med Child Neuro

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From 1 January 1995 to 31 December 2004, 22 patients (13 males, nine females; age range 2‐12mo) with infantile spasms and cytomegalovirus (CMV) infection were treated with intravenous ganciclovir (GCV) and antiepileptic drugs. GCV was given for 3 to 12 weeks with a 1‐month interval (one, two, or three courses). Epileptic spasms occurred before (group A: eight patients), simultaneously (group B: eight patients), and after (group C: six patients) a diagnosis of human CMV (HCMV) infection and antiviral treatment. In 11 patients, DNA HCMV was found in cerebrospinal fluid by nested‐polymerase chain reaction method (neuroinfection). All infants excreted CMV in urine. DNA HCMV and specific immunoglobulin M and immunoglobulin G antibodies were present in blood. Ten patients, including four with neuroinfection, have been seizure‐free for at least the past 18 months. In two patients with neuroinfection, vigabatrin monotherapy was withdrawn after a 2 year 6 month seizure‐free period. Eighteen patients required antiepileptic drugs polytherapy, four of whom required additional adrenocorticotropic hormone (ACTH). Six patients on polytherapy were seizure‐free on follow‐up, two of whom were treated with ACTH, but no patient with hypsarrhythmia who required ACTH treatment was seizure‐free on follow‐up. In five patients, psychomotor development was normal, 16 had tetraplegia (Gross Motor Function Classification System [GMFCS] Level V), and one had diplegia (GMFCS Level III). Early antiviral and antiepileptic therapy could result in the long‐term cessation of seizures.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Infantile spasms and cytomegalovirus infection: antiviral and antiepileptic treatment

Dunin-Wąsowicz

Kasprzyk-Obara

Jurkiewicz

et al. 2007

Develop Med Child Neuro

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“…Kohyama et al studied the involvement of human CMV infections in conditions of neurological impairment in 45 neurologically affected children aged 1 month to 17 years by means of polymerase chain reactions. 16 Several serologic assays are used to detect the CMV-specific IgM antibodies both in the whole serum and serum fractions obtained by sucrose density gradient centrifugation or column chromatography. Detection and quantification of CMV DNA in blood can be achieved by PCR and hybridization technique.…”

Section: Fig-2: Ct Scan Of Brain Showed Ventricular Dilatation(left Amentioning

confidence: 99%

Congenital Cytomegalovirus Infection As Lissencephaly - A Case Report

Kundu

Akhter

et al. 2014

Bangladesh J Child Health

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“…CSF PCR analysis for HCMV-DNA has been shown to be specific for the diagnosis of nervous system involve ment. but a negative test result does not exclude late neu rological sequelae [85][86][87][88],…”

Section: Diagnosis Of Hcmv Infections Of the Nervous Systemmentioning

confidence: 99%

Cytomegalovirus Infections of the Nervous System

Cinque

Marenzi

Ceresa³

1997

Intervirology

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Experimental evidence and pathological observation indicate that human cytomegalovirus (HCMV) has a tropism for cells of the nervous system, including both neuronal and glial cells. As demonstrated in animal models, after a viremic phase, the virus may reach the brain, where it may cause mild infection or severe encephalitis. The nervous system is one of the principal target organs in congenital HCMV infections and in HCMV-infected AIDS patients. In the former case, mortality is high and neurological sequelae, such as mental retardation, are frequent; in the latter it may lead to a progressively wasting encephalopathy and death within a few weeks. The diagnosis of the nervous system manifestations due to HCMV can now rely upon the detection of HCMV DNA in cerebrospinal fluid by means of polymerase chain reaction. However, the current antiviral treatments of these complications are of limited effect.

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Human cytomegalovirus DNA in cerebrospinal fluid.

Abstract: To determine the involvement of human cytomegalovirus (CMV)

Cited by 12 publications

References 30 publications

Infantile spasms and cytomegalovirus infection: antiviral and antiepileptic treatment

Infantile spasms and cytomegalovirus infection: antiviral and antiepileptic treatment

Congenital Cytomegalovirus Infection As Lissencephaly - A Case Report

Cytomegalovirus Infections of the Nervous System

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