Human Cytochrome P450 2W1 Is Not Expressed in Adrenal Cortex and Is Only Rarely Expressed in Adrenocortical Carcinomas

Abstract

Human cytochome P450 2W1 (CYP2W1) enzyme is expressed in fetal colon and in colon tumors. The level of expression is higher in colon metastases than in the parent tumors and the enzyme is a possible drug target for treatment of colorectal cancer, as demonstrated in mouse xenograft studies. A previous study published in this journal reported that CYP2W1 is highly expressed in normal and transformed adrenal tissue. However, adrenal expression of CYP2W1 protein was not seen in previous studies in our research group. To clarify this inconsistency, we have used qRT-PCR and Western blotting with CYP2W1-specific antibodies to probe a panel of 27 adrenocortical carcinomas and 35 normal adrenal cortex samples. CYP2W1 mRNA expression is seen in all samples. However, significant CYP2W1 protein expression was found in only one tumor sample (a testosterone-producing adrenocortical carcinoma) and not in any normal tissue. Differences in the specificity of the CYP2W1 antibodies used in the two studies may explain the apparent discrepancy. We conclude that normal adrenal tissue lacks P450 2W1 enzyme expression; also, adrenocortical carcinomas generally do not express the enzyme. This information thus underline the colon cancer specificity of CYP2W1 enzyme expression and has implications for the development of anti-colon cancer therapies based on CYP2W1 as a drug target, since 2W1-dependent bioactivation of prodrugs for CYP2W1 will not take place in normal adrenal tissue or other non-transformed tissues.

Micro RNA‐4651 Serves as a Potential Biomarker for Prognosis When Selecting Hepatocellular Carcinoma Patients for Postoperative Adjuvant Transarterial Chemoembolization Therapy

“…Forty‐eight hours after SMMC‐7721 transfected with mature microRNA‐4651 mimics, its inhibitor, or null control cells were used for analyzing the amount of CYP2W1 messenger RNA (mRNA) and protein. CYP2W1 mRNA and protein were respectively quantitated using real‐time quantitative PCR and western blot methods as previously described …”

“…CYP2W1 mRNA and protein were respectively quantitated using real-time quantitative PCR and western blot methods as previously described. (33)…”

T cell‐derived exosomes containing cytokines induced keratinocytes apoptosis in oral lichen planus

“…The disparity between the downregulation of IL‐1β and IL‐5 in OLP T‐exos and the upregulated expression of their genes in the OLP T cells might be due to the following reasons. To begin with, the poor correlation between the mRNA levels and protein levels in cells has been generally reported (Chen et al., 2002; Ideker et al., 2001; Nolé et al., 2016; Wang et al., 2019). Since the protein level of IL‐1β, IL‐5, and many other cytokines in OLP peripheral T cells has not yet been clarified, it is possible that the IL‐1β and IL‐5 mRNAs in OLP T cells were increased while their corresponding proteins were decreased, further leading to the downregulation of IL‐1β and IL‐5 in exosomes.…”

“…The disparity between the downregulation of IL-1β and IL-5 in OLP T-exos and the upregulated expression of their genes in the OLP T cells might be due to the following reasons. To begin with, the poor correlation between the mRNA levels and protein levels in cells has been generally reported (Chen et al, 2002;Ideker et al, 2001;Nolé et al, 2016;Wang et al, 2019). Since the protein level of IL- In contrast to the increased death of keratinocytes, the reduced or absent apoptosis of inflammatory cells has been suggested in OLP (Sugerman et al, 2002).…”