Human Corneal Endothelial Cells Expanded <i>In Vitro</i> Are a Powerful Resource for Tissue Engineering

Liu, Yongsong; Sun, Hong; Hu, Min; Zhu, Min; Tighe, Sean; Chen, Shuangling; Zhang, Yuan; Su, Chen‐Wei; Cai, Subo; Guo, Ping

doi:10.7150/ijms.17624

Cited by 24 publications

(16 citation statements)

References 66 publications

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“…However, creating an endothelial sheet (ES) might be the most important challenge to overcome. Unlike murine, rabbit, and bovine cells, human CECs have limited proliferative capability in vivo . Although decellularized and layered human corneal lamellae have shown biocompatibility with human CECs, making endothelialized human lamellae is a feasible option for endothelial keratoplasty in the future.…”

Section: Recellularizationmentioning

confidence: 99%

“…Unlike murine, rabbit, and bovine cells, human CECs have limited proliferative capability in vivo. 56 Although decellularized and layered human corneal lamellae have shown biocompatibility with human CECs, making endothelialized human lamellae is a feasible option for endothelial keratoplasty in the future. Currently, re-endothelialization by human CECs is only possible in vitro.…”

Section: Recellul Ariz Ationmentioning

confidence: 99%

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Decellularization methods for developing porcine corneal xenografts and future perspectives

Isidan

Liu

et al. 2019

Xenotransplantation

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Corneal transplantation is the only option to cure corneal opacities. However, there is an imbalance between supply and demand of corneal tissues in the world. To solve the problem of corneal shortage, corneal xenotransplantation studies have been implemented in the past years using porcine corneas. The corneal xenografts could come from (a) wild‐type pigs, (b) genetically engineered pigs, (c) decellularized porcine corneas, and (d) decellularized porcine corneas that are recellularized with human corneal cells, eventually with patients' own cells, as in all type of xenografts. All approaches except, the former would reduce or mitigate recipient immune responses. Although several techniques in decellularization have been reported, there is still no standardized protocol for the complete decellularization of corneal tissue. Herein, we reviewed different decellularization methods for porcine corneas based on the mechanism of action, decellularization efficacy, biocompatibility, and the undesirable effects on corneal ultrastructure. We compared 9 decellularization methods including: (a) sodium dodecyl sulfate, (b) triton x‐100, (c) hypertonic saline, (d) human serum with electrophoresis, (e) high hydrostatic pressure, (f) freeze‐thaw, (h) nitrogen gas, (h) phospholipase A2, and (i) glycerol with chemical crosslinking methods. It appears that combined methods could be more useful to perform efficient corneal decellularization.

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Section: Recellularizationmentioning

confidence: 99%

Section: Recellul Ariz Ationmentioning

confidence: 99%

Decellularization methods for developing porcine corneal xenografts and future perspectives

Isidan

Liu

et al. 2019

Xenotransplantation

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“…Despite the known importance of Collagen IV, it remains unclear of the mechanism of action in how it promotes cell attachment and growth on atelocollagen sheets. It also remains unclear how collagen IV may affect the behavior of HCEC aggregates (not single cells) such as phenotype on plastics [10][11][12][13][14][15][16][17][18] on atelocollagen sheets.…”

Section: Ivyspringmentioning

confidence: 99%

Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells

et al. 2019

Self Cite

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Corneal endothelial tissue engineering aims to find solutions for blindness due to endothelial dysfunction. A suitable combination of endothelial cells, substrates and environmental cues should be deployed for engineering functional endothelial tissues. This manuscript reviews up-to-date topics of corneal endothelial tissue engineering with special emphasis on biomaterial substrates and their properties, efficacy, and mechanisms of supporting functional endothelial cells in vitro.

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“…It plays a significant role in maintaining visual function: controls cornea hydration and transfers nutrients and other molecules from the aqueous humour [9]. As a result of aging, diseases, injury, or surgeries EC could be damaged and influence corneal blindness [10]. The minimum density of corneal EC for normal functioning is thought to be around 500 cells/mm [11].…”

Section: Introductionmentioning

confidence: 99%

Corneal Langerhans Cell And Endothelium Changes In Patients With Herpetic Keratitis During Acute Phase Of The Disease And After 6 Months

Danileviciene¹,

Žemaitienė²,

Vm³

et al. 2019

Preprint

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Purpose. To describe corneal morphological changes by evaluating Langerhans cell (LC) and endothelium cell density using in vivo laser scanning confocal microscopy (LSCM) in herpes simplex virus (HSV) keratitis during acute phase of the disease and after 6 months, to compare with contralateral eyes, controls with previous history of herpes labialis and healthy controls. Methods A prospective clinical study included 79 patients with herpetic eye disease, 101 healthy patients with previous history of herpes labialis but no history of herpetic eye disease, and 89 patients with no history of any HSV diseases. All patients underwent a complete ophthalmological examination including LSCM of the central cornea, using the Heidelberg Retina Tomograph III Rostock Cornea Module. After 6 months, the same ophthalmological examination was performed for patients with herpetic eye disease. Serology tests of the serum to detect HSV 1/2 IgG and IgM using Virion Serion ELISA classic kit were performed. Results HSV affected eyes showed an increase in LC density when compared with contralateral eyes, herpes labialis, and healthy control groups (357.9±308.7 vs. 197.1±258.5 vs. 62.9±72.6 vs. 81.7±88.3) (p<0.05). Higher LC density and less density of corneal sub-basal nerve parameters were found (p<0.05). Endothelium density in HSV affected eyes did not differ from the contralateral eyes, but it was lower when comparing with herpes labialis patients and healthy controls (2300.7±530.6 vs. 2343.3±543.7 vs. 2672.9±268.1 vs. 2620.9±347.1) (p<0.05). After 6 months, LC density in HSV affected eyes decreased but did not reach those of healthy controls (p<0.05). Conclusions LSCM reveals a significant increase of LC and decrease of endothelium cell density in HSV affected corneas. The decrease in LC density is a clear, indirect sign of recovery, which can be used in clinical practice.

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Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful Resource for Tissue Engineering

Cited by 24 publications

References 66 publications

Decellularization methods for developing porcine corneal xenografts and future perspectives

Decellularization methods for developing porcine corneal xenografts and future perspectives

Cellular Substrates for Cell-Based Tissue Engineering of Human Corneal Endothelial Cells

Corneal Langerhans Cell And Endothelium Changes In Patients With Herpetic Keratitis During Acute Phase Of The Disease And After 6 Months

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