Human Cord Blood‐Derived CD133+/C‐Kit+/Lin− Cells Have Bipotential Ability to Differentiate into Mesenchymal Stem Cells and Outgrowth Endothelial Cells

Cárdenas, Carlos; Kwon, Ja Young; Maeng, Yong Sun

doi:10.1155/2016/7162160

“…Indeed, CD34 + c-Kit + populations have already been c-Kit − cells among the CD34 + CD45 dim CD133 + population. g Illustration of CD45 and CD133 expressions according distinct patterns for CD34 and KDR of live lineage negative cells in peripheral blood of COVID-19 patients -Among the live cells, each of the 4 populations based on respective expressions of CD34 and KDR are phenotype on the CD45 and CD133 markers thus illustrating that all the KDR + events are exclusively CD45 high CD133 -*p < 0.05; **p < 0.01. described to have endothelial differentiation potential in vivo [20] and CD133 + c-Kit + lin − cells from human cord blood have also already been described to have ability to differentiate in ECFCs [21]. Potential mobilization of these cells in critical COVID-19 could provide endothelial regeneration helpful during hypoxia observed in COVID-19 patients [22,23], but on the other hand could participate to dissemination of thrombotic profile observed in clinic [8].…”

Section: Discussionmentioning

confidence: 99%

Multidimensional Proteomic Approach of Endothelial Progenitors Demonstrate Expression of KDR Restricted to CD19 Cells

Guerin

¹

,

Guyonnet

²

,

Goudot

³

et al. 2020

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Endothelial progenitor cells (EPCs) are involved in vasculogenesis and cardiovascular diseases. However, the phenotype of circulating EPCs remains elusive but they are more often described as CD34 + KDR + . The aim of the study was to extensively characterize circulating potential vasculogenic stem cell candidates in two populations of patients with cardiovascular disease by powerful multidimensional single cell complementary cytometric approaches (mass, imaging and flow). We identified cellular candidates in one patient before and after bioprosthetic total artificial heart implantation and results were confirmed in healthy peripheral and cord blood by mass cytometry. We also quantified cellular candidates in 10 patients with different COVID-19 severity. Both C-TAH implantation and COVID-19 at critical stage induce a redistribution of circulating CD34 + and CD19 + sub-populations in peripheral blood. After C-TAH implantation, circulating CD34 + progenitor cells expressed c-Kit stem marker while specific subsets CD34 + CD133 −/+ CD45 −/dim c-Kit + KDR − were mobilized. KDR was only expressed by CD19 + B-lymphocytes and CD14 + monocytes subpopulations in circulation. We confirmed by mass cytometry this KDR expression on CD19 + in healthy peripheral and cord blood, also with a VE-cadherin expression, confirming absence of endothelial lineage marker on CD34 + subtypes. In COVID-19, a significant mobilization of CD34 + c-Kit + KDR − cells was observed between moderate and critical COVID-19 patients regardless CD133 or CD45 expression. In order to better evaluate EPC phenotype, we performed imaging flow cytometry measurements of immature CD34 + KDR + cells in cord blood and showed that, after elimination of non-circular events, those cells were all CD19 + . During COVID-19, a significant mobilization of CD19 + KDR + per million of CD45 + cells was observed between moderate and critical COVID-19 patients regardless of CD34 expression. CD34 + c-Kit + cells are mobilized in both cardiovascular disease described here. KDR cells in peripheral blood are CD19 positive cells and are not classic vasculogenic stem and/or progenitor cells. A better evaluation of c-Kit and KDR expressing cells will lead to the redefinition of circulating endothelial progenitors. Graphical abstract Central illustration figure. Multidimensional proteomic approach of endothelial progenitors demonstrate expression of KDR restricted to CD19 cells. ...

show abstract

“…Our previous study showed that cord blood-derived mononuclear cells (MNCs) can differentiate into MSCs or outgrowth endothelial cells (OECs) [11]. We also characterized the differentiation potential of CD133 + /Ckit + /Lin − MNCs (CKL − cells) isolated from human umbilical cord blood (UCB) and confirmed that CKL − cells spontaneously differentiate into MSCs or OECs by performing RT-PCR and immunofluorescence staining for the respective specific markers [11].…”

Section: Backgroundsmentioning

confidence: 66%

“…Our previous study showed that cord blood-derived mononuclear cells (MNCs) can differentiate into MSCs or outgrowth endothelial cells (OECs) [11]. We also characterized the differentiation potential of CD133 + /Ckit + /Lin − MNCs (CKL − cells) isolated from human umbilical cord blood (UCB) and confirmed that CKL − cells spontaneously differentiate into MSCs or OECs by performing RT-PCR and immunofluorescence staining for the respective specific markers [11]. Based on the reported beneficial effects of human MSCs against sepsis [9,10], we hypothesized that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) could alleviate sepsis-associated acute organ and systemic inflammation via their immunomodulatory properties to improve survival in LPS-induced sepsis.…”

Section: Backgroundsmentioning

confidence: 99%

See 1 more Smart Citation

In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis

Sk

¹

,

Maeng

²

,

Kim

³

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background: Sepsis is a global inflammatory disease that causes death. It has been reported that mesenchymal stem cell (MSC) treatment can attenuate inflammatory and septic symptoms. In this study, we investigated how interactions between neutrophils and human umbilical cord blood (hUCB)-MSCs in the liver of septic mice are involved in mitigating sepsis that is mediated by MSCs. Accordingly, we aimed to determine whether hUCB-MSC application could be an appropriate treatment for sepsis. Methods: To induce septic condition, lipopolysaccharide (LPS) was intraperitoneally (i.p.) injected into mice 24 h after the intravenous (i.v.) injection of saline or hUCB-MSCs. To determine the effect of hUCB-MSCs on the immune response during sepsis, histologic analysis, immunoassays, and two-photon intravital imaging were performed 6 h post-LPS injection. For the survival study, mice were monitored for 6 days after LPS injection. Results: The injection (i.v.) of hUCB-MSCs alleviated the severity of LPS-induced sepsis by increasing IL-10 levels (p < 0.001) and decreasing mortality (p < 0.05) in septic mice. In addition, this significantly reduced the recruitment of neutrophils (p < 0.001) to the liver. In hUCB-MSC-treated condition, we also observed several distinct patterns of dynamic interactions between neutrophils and hUCB-MSCs in the inflamed mouse liver, as well as vigorous interactions between hepatic stellate cells (HSCs or ito cells) and hUCB-MSCs. Interestingly, hUCB-MSCs that originated from humans were not recognized as foreign in the mouse body and consequently did not cause graft rejection. Conclusions: These distinct interaction patterns between innate immune cells and hUCB-MSCs demonstrated that hUCB-MSCs have beneficial effects against LPS-induced sepsis through associations with neutrophils. In addition, the immunomodulatory properties of hUCB-MSCs might enable immune evasion in the host. Taken together, our results suggest the prospects of hUCB-MSCs as a therapeutic tool to inhibit inflammation and alleviate pathological immune responses such as sepsis.

show abstract

“…Cells were fixed in 4% formaldehyde, dehydrated in an ethanol series, and embedded in paraffin blocks. Blocks were cut, and sections were stained for sulphated proteoglycans with Safranin O (0.1% aqueous solution) (Sigma-Aldrich) to evaluate chondrogenic differentiation [43,44].…”

Section: Flow Cytometric Analysismentioning

confidence: 99%

Comparative Analysis of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells between Preeclampsia and Normal Pregnant Women

Hwang

¹

,

Maeng

²

2020

Stem Cells International

Self Cite

View full text Add to dashboard Cite

Preeclampsia is a syndrome characterized by deterioration of either the maternal condition or the fetal condition. The adverse intrauterine environment made by preeclampsia results into intrauterine growth restriction and increased risk of a variety of diseases in future life. Given the adverse environment of fetal circulation made in the preeclamptic condition, and the role of mesenchymal stem cell (MSC) as a multipotent progenitor cell, we hypothesized that MSCs derived from human umbilical cord blood (hUCB-MSCs) obtained from preeclampsia are adversely altered or affected compared with normal pregnancy. The aim of this study was to analyze the biological characteristics and compare the functional abilities and gene expression patterns of hUCB-MSCs originating from pregnant women with and without severe preeclampsia. hUCB-MSCs were isolated and cultured from 28 pregnant women with severe preeclampsia and 30 normal pregnant women. hUCB-MSCs obtained from women with preeclampsia were less proliferative and more senescent and had lower telomerase activity and higher ROS activity than cells from women with normal pregnancy. In addition, many senescence-related differentially expressed genes (DEGs) were identified by analysis of microarray gene expression profiles and significantly associated with the Gene Ontology term cell aging. In conclusion, hUCB-MSCs obtained from women with preeclampsia showed the poorly proliferative, more senescent, and decreased telomerase activity, and these characters may be related with functional impairment of MSC from preeclampsia compared with cells from normal pregnancy.

show abstract

Human Cord Blood‐Derived CD133⁺/C‐Kit⁺/Lin⁻ Cells Have Bipotential Ability to Differentiate into Mesenchymal Stem Cells and Outgrowth Endothelial Cells

Cited by 9 publications

References 45 publications

Multidimensional Proteomic Approach of Endothelial Progenitors Demonstrate Expression of KDR Restricted to CD19 Cells

Multidimensional Proteomic Approach of Endothelial Progenitors Demonstrate Expression of KDR Restricted to CD19 Cells

In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis

Comparative Analysis of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells between Preeclampsia and Normal Pregnant Women

Contact Info

Product

Resources

About