Human circulating ribosomal DNA content significantly increases while circulating satellite III (1q12) content decreases under chronic occupational exposure to low-dose gamma- neutron and tritium beta-radiation

Korzeneva, Inna B.; Kostuyk, Svetlana V.; Ershova, Elizaveta S.; Skorodumova, E.; Zhuravleva, Veronika F.; Pankratova, Galina V.; Volkova, Irina V.; Степанова, Елена В.; Porokhovnik, Lev N.; Veiko, Natalia N.

doi:10.1016/j.mrfmmm.2016.09.001

Cited by 26 publications

(37 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As we showed previously, quantification of damaged rDNA produced better results when the method of hybridization with oligonucleotide or long DNA probes was used in the non-radioactive variant ( Korzeneva et al, 2016 ; Chestkov et al, 2018 ). Unlike the generally acknowledged qPCR, NQH technique is not applicable to unique (single-copy) genes.…”

Section: Resultsmentioning

confidence: 53%

“…At present, the main method for the quantification of various genome sequences is the generally acknowledge technique of qPCR. However, the rRNA-coding region of rDNA is a problematic object for the quantification using qPCR for several reasons, which are discussed in detail in our previous articles ( Korzeneva et al, 2016 ; Chestkov et al, 2018 ). Briefly, the qPCR efficiency is affected by the tandem nature of rDNA repeats and the presence of self-complementary regions within the repeat unit, the heterogeneity of rDNA copies with regard to methylation.…”

Section: Resultsmentioning

confidence: 99%

“…It was specified in details previously in three publications ( Veiko et al, 2003 ; Korzeneva et al, 2016 ; Chestkov et al, 2018 ). Briefly, the denatured DNA samples (from 4 to 6 dots per each sample) were applied to a filter (Optitran BA-S85, GE healthcare).…”

Section: Methodsmentioning

confidence: 99%

“…Our most recent studies have shown that the method of non-radioactive quantitative hybridization (NQH) yields more accurate and reproducible results for rDNA content, than qPCR. The difference between the two techniques are especially prominent when assaying damaged DNA samples [DNA derived from old cells, from the patients with high level of oxidative stress, oxidized DNA ( Chestkov et al, 2018 ), and cell-free DNA ( Korzeneva et al, 2016 )].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Copy Number of Human Ribosomal Genes With Aging: Unchanged Mean, but Narrowed Range and Decreased Variance in Elderly Group

et al. 2018

Self Cite

View full text Add to dashboard Cite

Introduction: The multi-copied genes coding for the human 18, 5.8, and 28S ribosomal RNA (rRNA) are located in five pairs of acrocentric chromosomes forming so-called rDNA. Human genome contains unmethylated, slightly methylated, and hypermethylated copies of rDNA. The major research question: What is the rDNA copy number (rDNA CN) and the content of hypermethylated rDNA as a function of age?Materials and Methods: We determined the rDNA CN in the blood leukocyte genomes of 651 subjects aged 17 to 91 years. The subjects were divided into two subgroups: “elderly” group (E-group, N = 126) – individuals over 72 years of age (the age of the population’s mean lifetime for Russia) and “non-elderly” group (NE-group, N = 525). The hypermethylated rDNA content was determined in the 40 DNA samples from the each group. The change in rDNA during replicative cell senescence was studied for the cultured skin fibroblast lines of five subjects from NE-group. Non-radioactive quantitative dot- and blot-hybridization techniques (NQH) were applied.Results: In the subjects from the E-group the mean rDNA CN was the same, but the range of variation was narrower compared to the NE-group: a range of 272 to 541 copies in E-group vs. 200 to 711 copies in NE-group. Unlike NE-group, the E-group genomes contained almost no hypermethylated rDNA copies. A case study of cultured skin fibroblasts from five subjects has shown that during the replicative senescence the genome lost hypermethylated rDNA copies only.Conclusion: In the elderly group, the mean rDNA CN is the same, but the range of variation is narrower compared with the younger subjects. During replicative senescence, the human fibroblast genome loses hypermethylated copies of rDNA. Two hypotheses were put forward: (1) individuals with either very low or very high rDNA content in their genomes do not survive till the age of the population’s mean lifetime; and/or (2) during the aging, the human genome eliminates hypermethylated copies of rDNA.

show abstract

Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Copy Number of Human Ribosomal Genes With Aging: Unchanged Mean, but Narrowed Range and Decreased Variance in Elderly Group

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…It should be noted that GC-rich fragments within genomic DNA tend to accumulate oxidative damage as well. We have previously demonstrated that chronic exposure to gamma-neutron or tritium β -radiation evokes an increase in the content of GC-rich sequences (69% GC) in the transcribed region of human ribosomal repeat (rDNA) in cfDNA from 166 individuals [ 50 ]. The reason for this phenomenon is the increased stability of GC repeats to hydrolysis [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response

Sergeeva

Ershova

Veiko

et al. 2017

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

We have hypothesized that the adaptive response to low doses of ionizing radiation (IR) is mediated by oxidized cell-free DNA (cfDNA) fragments. Here, we summarize our experimental evidence for this model. Studies involving measurements of ROS, expression of the NOX (superoxide radical production), induction of apoptosis and DNA double-strand breaks, antiapoptotic gene expression and cell cycle inhibition confirm this hypothesis. We have demonstrated that treatment of mesenchymal stem cells (MSCs) with low doses of IR (10 cGy) leads to cell death of part of cell population and release of oxidized cfDNA. cfDNA has the ability to penetrate into the cytoplasm of other cells. Oxidized cfDNA, like low doses of IR, induces oxidative stress, ROS production, ROS-induced oxidative modifications of nuclear DNA, DNA breaks, arrest of the cell cycle, activation of DNA reparation and antioxidant response, and inhibition of apoptosis. The MSCs pretreated with low dose of irradiation or oxidized cfDNA were equally effective in induction of adaptive response to challenge further dose of radiation. Our studies suggest that oxidized cfDNA is a signaling molecule in the stress signaling that mediates radiation-induced bystander effects and that it is an important component of the development of radioadaptive responses to low doses of IR.

show abstract

A systematic literature review and meta-analysis of circulating nucleic acids as biomarkers in psychiatry

Verebi

Nectoux

Gorwood

et al. 2023

Progress in Neuro-Psychopharmacology and Biological Psychiatry

View full text Add to dashboard Cite

Human circulating ribosomal DNA content significantly increases while circulating satellite III (1q12) content decreases under chronic occupational exposure to low-dose gamma- neutron and tritium beta-radiation

Cited by 26 publications

References 34 publications

Copy Number of Human Ribosomal Genes With Aging: Unchanged Mean, but Narrowed Range and Decreased Variance in Elderly Group

Copy Number of Human Ribosomal Genes With Aging: Unchanged Mean, but Narrowed Range and Decreased Variance in Elderly Group

Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response

A systematic literature review and meta-analysis of circulating nucleic acids as biomarkers in psychiatry

Contact Info

Product

Resources

About